Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle

Autores
Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; Da Rocha, David Gitirana; Da Silva, Wilmar Dias; Lasunskaia, Elena B.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Materia
APA PROTEIN
FECAL BIOMARKER
IMMUNOCHEMICAL METHODS
PARATUBERCULOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85058
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85058
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattleDa Silva De Souza, GilianeRodriguez, Ana Barbara F.Romano, Maria IsabelRibeiro, Efranci SeliprandyOelemann, Walter M. R.Da Rocha, David GitiranaDa Silva, Wilmar DiasLasunskaia, Elena B.APA PROTEINFECAL BIOMARKERIMMUNOCHEMICAL METHODSPARATUBERCULOSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; BrasilFil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilOxford University Press2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85058Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-82049-632XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/76/6/fty063/5057472info:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/fty063info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:59Zoai:ri.conicet.gov.ar:11336/85058instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:59.333CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
title Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
spellingShingle Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
Da Silva De Souza, Giliane
APA PROTEIN
FECAL BIOMARKER
IMMUNOCHEMICAL METHODS
PARATUBERCULOSIS
title_short Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
title_full Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
title_fullStr Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
title_full_unstemmed Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
title_sort Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
dc.creator.none.fl_str_mv Da Silva De Souza, Giliane
Rodriguez, Ana Barbara F.
Romano, Maria Isabel
Ribeiro, Efranci Seliprandy
Oelemann, Walter M. R.
Da Rocha, David Gitirana
Da Silva, Wilmar Dias
Lasunskaia, Elena B.
author Da Silva De Souza, Giliane
author_facet Da Silva De Souza, Giliane
Rodriguez, Ana Barbara F.
Romano, Maria Isabel
Ribeiro, Efranci Seliprandy
Oelemann, Walter M. R.
Da Rocha, David Gitirana
Da Silva, Wilmar Dias
Lasunskaia, Elena B.
author_role author
author2 Rodriguez, Ana Barbara F.
Romano, Maria Isabel
Ribeiro, Efranci Seliprandy
Oelemann, Walter M. R.
Da Rocha, David Gitirana
Da Silva, Wilmar Dias
Lasunskaia, Elena B.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APA PROTEIN
FECAL BIOMARKER
IMMUNOCHEMICAL METHODS
PARATUBERCULOSIS
topic APA PROTEIN
FECAL BIOMARKER
IMMUNOCHEMICAL METHODS
PARATUBERCULOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
description Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85058
Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-8
2049-632X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85058
identifier_str_mv Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-8
2049-632X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/76/6/fty063/5057472
info:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/fty063
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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