Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle
- Autores
- Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; Da Rocha, David Gitirana; Da Silva, Wilmar Dias; Lasunskaia, Elena B.
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.
Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil
Fil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil - Materia
-
APA PROTEIN
FECAL BIOMARKER
IMMUNOCHEMICAL METHODS
PARATUBERCULOSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/85058
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattleDa Silva De Souza, GilianeRodriguez, Ana Barbara F.Romano, Maria IsabelRibeiro, Efranci SeliprandyOelemann, Walter M. R.Da Rocha, David GitiranaDa Silva, Wilmar DiasLasunskaia, Elena B.APA PROTEINFECAL BIOMARKERIMMUNOCHEMICAL METHODSPARATUBERCULOSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis.Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; BrasilFil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilFil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; BrasilOxford University Press2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85058Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-82049-632XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/76/6/fty063/5057472info:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/fty063info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:59Zoai:ri.conicet.gov.ar:11336/85058instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:00.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
title |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
spellingShingle |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle Da Silva De Souza, Giliane APA PROTEIN FECAL BIOMARKER IMMUNOCHEMICAL METHODS PARATUBERCULOSIS |
title_short |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
title_full |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
title_fullStr |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
title_full_unstemmed |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
title_sort |
Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle |
dc.creator.none.fl_str_mv |
Da Silva De Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. Da Rocha, David Gitirana Da Silva, Wilmar Dias Lasunskaia, Elena B. |
author |
Da Silva De Souza, Giliane |
author_facet |
Da Silva De Souza, Giliane Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. Da Rocha, David Gitirana Da Silva, Wilmar Dias Lasunskaia, Elena B. |
author_role |
author |
author2 |
Rodriguez, Ana Barbara F. Romano, Maria Isabel Ribeiro, Efranci Seliprandy Oelemann, Walter M. R. Da Rocha, David Gitirana Da Silva, Wilmar Dias Lasunskaia, Elena B. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
APA PROTEIN FECAL BIOMARKER IMMUNOCHEMICAL METHODS PARATUBERCULOSIS |
topic |
APA PROTEIN FECAL BIOMARKER IMMUNOCHEMICAL METHODS PARATUBERCULOSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. Fil: Da Silva De Souza, Giliane. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil Fil: Rodriguez, Ana Barbara F.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ribeiro, Efranci Seliprandy. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil Fil: Oelemann, Walter M. R.. Universidade Federal do Rio de Janeiro; Brasil Fil: Da Rocha, David Gitirana. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil Fil: Da Silva, Wilmar Dias. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil Fil: Lasunskaia, Elena B.. Universidade Estadual Do Norte Fluminense Darcy Ribeiro; Brasil |
description |
Paratuberculosis (PTB) or Johne's disease is a chronic intestinal infection of ruminants, caused by Mycobacterium avium subsp. paratuberculosis. The shedding of mycobacteria in the feces starts at the initial stages and increases with disease progression, suggesting that antigens secreted by mycobacteria could be excreted in the feces. Previously, we demonstrated that the alanine and proline-rich antigen (Apa), a secretory antigen of Map, could be detected in the intestine of cows with PTB using a monoclonal antibody. In this study, we verified whether this protein can be found in consistently detectable levels in the feces of cattle with PTB. Feces were obtained from cows with Johne's disease confirmed by laboratory tests, cows with suspected PTB based on seropositivity and from PTB-free control cows. Samples were immunoprecipitated using anti-Apa monoclonal antibody and analyzed by immunoblot. The Apa was detected as a 60/70 kDa doublet band in all samples obtained from animals with laboratory-confirmed disease and in a substantial proportion of seropositive asymptomatic animals, but not in the control samples. Additionally, the antigen was detected in the feces of animals with Johne's disease by ELISA. This study strongly suggests that Apa is a potential fecal biomarker of Johne's disease that could serve for immunodiagnosis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/85058 Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-8 2049-632X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/85058 |
identifier_str_mv |
Da Silva De Souza, Giliane; Rodriguez, Ana Barbara F.; Romano, Maria Isabel; Ribeiro, Efranci Seliprandy; Oelemann, Walter M. R.; et al.; Identification of the apa protein secreted by Mycobacterium avium subsp. paratuberculosis as a novel fecal biomarker for Johne's disease in cattle; Oxford University Press; Pathogens and Disease; 76; 6; 8-2018; 1-8 2049-632X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femspd/article/76/6/fty063/5057472 info:eu-repo/semantics/altIdentifier/doi/10.1093/femspd/fty063 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.13397 |