A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides

Autores
Jacquat, Andrés Gustavo; Theumer, Martín Gustavo; Cañizares, María Carmen; Debat, Humberto Julio; Iglesias, Juliana; García Pedrajas, María Dolores; Dambolena, José Sebastián
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
EEA Pergamino
Fil: Jacquat, Andrés Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas . Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
Fil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fuente
Viruses 12 (10) : 1161 (October 2020)
Materia
Maíz
Gramíneas Forrajeras
Sorgos
Hongos
Fusarium
Maize
Feed Grasses
Sorghum Grain
Fungi
Mitovirus
Mycovirus
Fusarium verticillioides
Fusarium andiyazi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8135
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8135
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioidesJacquat, Andrés GustavoTheumer, Martín GustavoCañizares, María CarmenDebat, Humberto JulioIglesias, JulianaGarcía Pedrajas, María DoloresDambolena, José SebastiánMaízGramíneas ForrajerasSorgosHongosFusariumMaizeFeed GrassesSorghum GrainFungiMitovirusMycovirusFusarium verticillioidesFusarium andiyaziMycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.EEA PergaminoFil: Jacquat, Andrés Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas . Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; EspañaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; ArgentinaFil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; EspañaFil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; ArgentinaMDPI2020-10-27T16:11:06Z2020-10-27T16:11:06Z2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8135https://www.mdpi.com/1999-4915/12/10/11611999-4915https://doi.org/10.3390/v12101161Viruses 12 (10) : 1161 (October 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:03Zoai:localhost:20.500.12123/8135instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.46INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
title A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
spellingShingle A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
Jacquat, Andrés Gustavo
Maíz
Gramíneas Forrajeras
Sorgos
Hongos
Fusarium
Maize
Feed Grasses
Sorghum Grain
Fungi
Mitovirus
Mycovirus
Fusarium verticillioides
Fusarium andiyazi
title_short A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
title_full A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
title_fullStr A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
title_full_unstemmed A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
title_sort A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
dc.creator.none.fl_str_mv Jacquat, Andrés Gustavo
Theumer, Martín Gustavo
Cañizares, María Carmen
Debat, Humberto Julio
Iglesias, Juliana
García Pedrajas, María Dolores
Dambolena, José Sebastián
author Jacquat, Andrés Gustavo
author_facet Jacquat, Andrés Gustavo
Theumer, Martín Gustavo
Cañizares, María Carmen
Debat, Humberto Julio
Iglesias, Juliana
García Pedrajas, María Dolores
Dambolena, José Sebastián
author_role author
author2 Theumer, Martín Gustavo
Cañizares, María Carmen
Debat, Humberto Julio
Iglesias, Juliana
García Pedrajas, María Dolores
Dambolena, José Sebastián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Maíz
Gramíneas Forrajeras
Sorgos
Hongos
Fusarium
Maize
Feed Grasses
Sorghum Grain
Fungi
Mitovirus
Mycovirus
Fusarium verticillioides
Fusarium andiyazi
topic Maíz
Gramíneas Forrajeras
Sorgos
Hongos
Fusarium
Maize
Feed Grasses
Sorghum Grain
Fungi
Mitovirus
Mycovirus
Fusarium verticillioides
Fusarium andiyazi
dc.description.none.fl_txt_mv Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
EEA Pergamino
Fil: Jacquat, Andrés Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas . Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina
Fil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España
Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-27T16:11:06Z
2020-10-27T16:11:06Z
2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8135
https://www.mdpi.com/1999-4915/12/10/1161
1999-4915
https://doi.org/10.3390/v12101161
url http://hdl.handle.net/20.500.12123/8135
https://www.mdpi.com/1999-4915/12/10/1161
https://doi.org/10.3390/v12101161
identifier_str_mv 1999-4915
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Viruses 12 (10) : 1161 (October 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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