Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance

Autores
Campos Bermudez, Valeria Alina; Fauguel, Carolina María; Tronconi, Marcos Ariel; Casati, Paula; Presello, Daniel; Andreo, Carlos Santiago
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
Fil: Campos Bermudez, Valeria Alina. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Fauguel, Carolina María. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;
Fil: Tronconi, Marcos Ariel. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Casati, Paula. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Presello, Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;
Fil: Andreo, Carlos Santiago. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Materia
Fusarium verticillioides
maiz
microarrays
metabolomica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/510
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/510
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot ResistanceCampos Bermudez, Valeria AlinaFauguel, Carolina MaríaTronconi, Marcos ArielCasati, PaulaPresello, DanielAndreo, Carlos SantiagoFusarium verticillioidesmaizmicroarraysmetabolomicahttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.Fil: Campos Bermudez, Valeria Alina. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);Fil: Fauguel, Carolina María. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;Fil: Tronconi, Marcos Ariel. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);Fil: Casati, Paula. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);Fil: Presello, Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;Fil: Andreo, Carlos Santiago. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);Public Library Science2013-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/510Campos Bermudez, Valeria Alina; Fauguel, Carolina María; Tronconi, Marcos Ariel; Casati, Paula; Presello, Daniel; Andreo, Carlos Santiago; Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance; Public Library Science; Plos One; 8; 4; 18-4-2013; 61580-61580;1932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0061580info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:33Zoai:ri.conicet.gov.ar:11336/510instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:33.385CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
title Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
spellingShingle Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
Campos Bermudez, Valeria Alina
Fusarium verticillioides
maiz
microarrays
metabolomica
title_short Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
title_full Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
title_fullStr Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
title_full_unstemmed Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
title_sort Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance
dc.creator.none.fl_str_mv Campos Bermudez, Valeria Alina
Fauguel, Carolina María
Tronconi, Marcos Ariel
Casati, Paula
Presello, Daniel
Andreo, Carlos Santiago
author Campos Bermudez, Valeria Alina
author_facet Campos Bermudez, Valeria Alina
Fauguel, Carolina María
Tronconi, Marcos Ariel
Casati, Paula
Presello, Daniel
Andreo, Carlos Santiago
author_role author
author2 Fauguel, Carolina María
Tronconi, Marcos Ariel
Casati, Paula
Presello, Daniel
Andreo, Carlos Santiago
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Fusarium verticillioides
maiz
microarrays
metabolomica
topic Fusarium verticillioides
maiz
microarrays
metabolomica
purl_subject.fl_str_mv https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
Fil: Campos Bermudez, Valeria Alina. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Fauguel, Carolina María. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;
Fil: Tronconi, Marcos Ariel. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Casati, Paula. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
Fil: Presello, Daniel. Instituto Nacional de Tecnologia Agropecuaria. Centro Reg.buenos Aires Norte. Estacion Exptal.agrop.pergamino;
Fil: Andreo, Carlos Santiago. Consejo Nacional de Invest.cientif.y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Centro de Est.fotosinteticos y Bioquimicos (i);
description Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/510
Campos Bermudez, Valeria Alina; Fauguel, Carolina María; Tronconi, Marcos Ariel; Casati, Paula; Presello, Daniel; Andreo, Carlos Santiago; Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance; Public Library Science; Plos One; 8; 4; 18-4-2013; 61580-61580;
1932-6203
url http://hdl.handle.net/11336/510
identifier_str_mv Campos Bermudez, Valeria Alina; Fauguel, Carolina María; Tronconi, Marcos Ariel; Casati, Paula; Presello, Daniel; Andreo, Carlos Santiago; Transcriptional and Metabolic Changes Associated to the Infection by Fusarium verticillioides in Maize Inbreds with Contrasting Ear Rot Resistance; Public Library Science; Plos One; 8; 4; 18-4-2013; 61580-61580;
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0061580
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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