A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides

Autores
Jacquat, Andrés Gustavo; Theumer, Martín Gustavo; Cañizares-Nolasco, María del Carmen; Debat, Humberto Julio; Iglesias, Juliana; García-Pedrajas, Maria Dolores; Dambolena, José Sebastián
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
Fil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cañizares-Nolasco, María del Carmen. Consejo Superior de Investigaciones Científicas; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: García-Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; España
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
MAIZE
SORGHUM
FUSARIUM VERTICILLIOIDES
FUSARIUM ANDIYAZI
MYCOVIRUS
MITOVIRUS
FVMV1
FAMV1-162
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118467
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/118467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioidesJacquat, Andrés GustavoTheumer, Martín GustavoCañizares-Nolasco, María del CarmenDebat, Humberto JulioIglesias, JulianaGarcía-Pedrajas, Maria DoloresDambolena, José SebastiánMAIZESORGHUMFUSARIUM VERTICILLIOIDESFUSARIUM ANDIYAZIMYCOVIRUSMITOVIRUSFVMV1FAMV1-162https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.Fil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cañizares-Nolasco, María del Carmen. Consejo Superior de Investigaciones Científicas; EspañaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: García-Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; EspañaFil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaMDPI AG2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118467Jacquat, Andrés Gustavo; Theumer, Martín Gustavo; Cañizares-Nolasco, María del Carmen; Debat, Humberto Julio; Iglesias, Juliana; et al.; A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides; MDPI AG; Viruses; 12; 10; 10-2020; 11611999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/12/10/1161info:eu-repo/semantics/altIdentifier/doi/10.3390/v12101161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:50Zoai:ri.conicet.gov.ar:11336/118467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:50.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
title A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
spellingShingle A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
Jacquat, Andrés Gustavo
MAIZE
SORGHUM
FUSARIUM VERTICILLIOIDES
FUSARIUM ANDIYAZI
MYCOVIRUS
MITOVIRUS
FVMV1
FAMV1-162
title_short A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
title_full A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
title_fullStr A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
title_full_unstemmed A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
title_sort A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides
dc.creator.none.fl_str_mv Jacquat, Andrés Gustavo
Theumer, Martín Gustavo
Cañizares-Nolasco, María del Carmen
Debat, Humberto Julio
Iglesias, Juliana
García-Pedrajas, Maria Dolores
Dambolena, José Sebastián
author Jacquat, Andrés Gustavo
author_facet Jacquat, Andrés Gustavo
Theumer, Martín Gustavo
Cañizares-Nolasco, María del Carmen
Debat, Humberto Julio
Iglesias, Juliana
García-Pedrajas, Maria Dolores
Dambolena, José Sebastián
author_role author
author2 Theumer, Martín Gustavo
Cañizares-Nolasco, María del Carmen
Debat, Humberto Julio
Iglesias, Juliana
García-Pedrajas, Maria Dolores
Dambolena, José Sebastián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv MAIZE
SORGHUM
FUSARIUM VERTICILLIOIDES
FUSARIUM ANDIYAZI
MYCOVIRUS
MITOVIRUS
FVMV1
FAMV1-162
topic MAIZE
SORGHUM
FUSARIUM VERTICILLIOIDES
FUSARIUM ANDIYAZI
MYCOVIRUS
MITOVIRUS
FVMV1
FAMV1-162
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
Fil: Jacquat, Andrés Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Theumer, Martín Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cañizares-Nolasco, María del Carmen. Consejo Superior de Investigaciones Científicas; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: García-Pedrajas, Maria Dolores. Consejo Superior de Investigaciones Científicas; España
Fil: Dambolena, José Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118467
Jacquat, Andrés Gustavo; Theumer, Martín Gustavo; Cañizares-Nolasco, María del Carmen; Debat, Humberto Julio; Iglesias, Juliana; et al.; A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides; MDPI AG; Viruses; 12; 10; 10-2020; 1161
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118467
identifier_str_mv Jacquat, Andrés Gustavo; Theumer, Martín Gustavo; Cañizares-Nolasco, María del Carmen; Debat, Humberto Julio; Iglesias, Juliana; et al.; A survey of mycoviral infection in Fusarium spp. Isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides; MDPI AG; Viruses; 12; 10; 10-2020; 1161
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/12/10/1161
info:eu-repo/semantics/altIdentifier/doi/10.3390/v12101161
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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