Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages

Autores
Fiorani, Franco; Lahiri, Priyoshi; Puentes, Rodrigo; Bradley, Peter John; Moore, Prando Dadin; Cobo, Eduardo Ruben
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infections with the protozoan Neospora caninum cause abortion in cattle, likely due to the parasite’s replication and excessive inflammation in the placenta. Cathelicidins are host defense peptides known for their antimicrobial and immunomodulatory functions, but their role in N. caninum infections remains elusive. Using bone marrow-derived macrophages (BMDMs) isolated from mice expressing (wild-type, Camp+/+) and lacking (Camp−/−) cathelicidins, we investigated the role of endogenous cathelicidin in infections with N. caninum. We show that Camp−/− macrophages primed with lipopolysaccharide (LPS) had an increased number of intracellular N. caninum tachyzoites, and these macrophages released higher amounts of IL-1β and lactate dehydrogenase (LDH), a marker of cytotoxicity. These findings indicate that cathelicidins contribute to intracellular N. caninum control and inflammation by limiting the activation of the inflammasome, particularly under LPS-induced conditions. This insight reveals the immunomodulatory role of cathelicidins in controlling N. caninum-associated pathologies.
EEA Balcarce
Fil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Lahiri, Priyoshi. University of Calgary; Canadá
Fil: Puentes, Rodrigo. University of Calgary; Canadá
Fil: Bradley, Peter John. University of California at Los Angeles; Estados Unidos
Fil: Moore, Dadin Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Cobo, Eduardo Ruben. University of Calgary; Canadá
Fuente
Pathogens 14 (7) : 663 (July 2025)
Materia
Neospora caninum
Macrophages
Peptides
Host Pathogen Relations
Immune Response
Macrófogos
Péptidos
Relación Huésped Patógeno
Respuesta Inmunológica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25115
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oai_identifier_str oai:localhost:20.500.12123/25115
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network_name_str INTA Digital (INTA)
spelling Cathelicidins Limit Intracellular Neospora caninum-Infection in MacrophagesFiorani, FrancoLahiri, PriyoshiPuentes, RodrigoBradley, Peter JohnMoore, Prando DadinCobo, Eduardo RubenNeospora caninumMacrophagesPeptidesHost Pathogen RelationsImmune ResponseMacrófogosPéptidosRelación Huésped PatógenoRespuesta InmunológicaInfections with the protozoan Neospora caninum cause abortion in cattle, likely due to the parasite’s replication and excessive inflammation in the placenta. Cathelicidins are host defense peptides known for their antimicrobial and immunomodulatory functions, but their role in N. caninum infections remains elusive. Using bone marrow-derived macrophages (BMDMs) isolated from mice expressing (wild-type, Camp+/+) and lacking (Camp−/−) cathelicidins, we investigated the role of endogenous cathelicidin in infections with N. caninum. We show that Camp−/− macrophages primed with lipopolysaccharide (LPS) had an increased number of intracellular N. caninum tachyzoites, and these macrophages released higher amounts of IL-1β and lactate dehydrogenase (LDH), a marker of cytotoxicity. These findings indicate that cathelicidins contribute to intracellular N. caninum control and inflammation by limiting the activation of the inflammasome, particularly under LPS-induced conditions. This insight reveals the immunomodulatory role of cathelicidins in controlling N. caninum-associated pathologies.EEA BalcarceFil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Lahiri, Priyoshi. University of Calgary; CanadáFil: Puentes, Rodrigo. University of Calgary; CanadáFil: Bradley, Peter John. University of California at Los Angeles; Estados UnidosFil: Moore, Dadin Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Cobo, Eduardo Ruben. University of Calgary; CanadáMultidisciplinary Digital Publishing Institute, MDPI2026-02-09T11:07:54Z2026-02-09T11:07:54Z2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25115https://www.mdpi.com/2076-0817/14/7/6632076-0817https://doi.org/10.3390/pathogens14070663Pathogens 14 (7) : 663 (July 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:41Zoai:localhost:20.500.12123/25115instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:41.738INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
title Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
spellingShingle Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
Fiorani, Franco
Neospora caninum
Macrophages
Peptides
Host Pathogen Relations
Immune Response
Macrófogos
Péptidos
Relación Huésped Patógeno
Respuesta Inmunológica
title_short Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
title_full Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
title_fullStr Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
title_full_unstemmed Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
title_sort Cathelicidins Limit Intracellular Neospora caninum-Infection in Macrophages
dc.creator.none.fl_str_mv Fiorani, Franco
Lahiri, Priyoshi
Puentes, Rodrigo
Bradley, Peter John
Moore, Prando Dadin
Cobo, Eduardo Ruben
author Fiorani, Franco
author_facet Fiorani, Franco
Lahiri, Priyoshi
Puentes, Rodrigo
Bradley, Peter John
Moore, Prando Dadin
Cobo, Eduardo Ruben
author_role author
author2 Lahiri, Priyoshi
Puentes, Rodrigo
Bradley, Peter John
Moore, Prando Dadin
Cobo, Eduardo Ruben
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Neospora caninum
Macrophages
Peptides
Host Pathogen Relations
Immune Response
Macrófogos
Péptidos
Relación Huésped Patógeno
Respuesta Inmunológica
topic Neospora caninum
Macrophages
Peptides
Host Pathogen Relations
Immune Response
Macrófogos
Péptidos
Relación Huésped Patógeno
Respuesta Inmunológica
dc.description.none.fl_txt_mv Infections with the protozoan Neospora caninum cause abortion in cattle, likely due to the parasite’s replication and excessive inflammation in the placenta. Cathelicidins are host defense peptides known for their antimicrobial and immunomodulatory functions, but their role in N. caninum infections remains elusive. Using bone marrow-derived macrophages (BMDMs) isolated from mice expressing (wild-type, Camp+/+) and lacking (Camp−/−) cathelicidins, we investigated the role of endogenous cathelicidin in infections with N. caninum. We show that Camp−/− macrophages primed with lipopolysaccharide (LPS) had an increased number of intracellular N. caninum tachyzoites, and these macrophages released higher amounts of IL-1β and lactate dehydrogenase (LDH), a marker of cytotoxicity. These findings indicate that cathelicidins contribute to intracellular N. caninum control and inflammation by limiting the activation of the inflammasome, particularly under LPS-induced conditions. This insight reveals the immunomodulatory role of cathelicidins in controlling N. caninum-associated pathologies.
EEA Balcarce
Fil: Fiorani, Franco. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Lahiri, Priyoshi. University of Calgary; Canadá
Fil: Puentes, Rodrigo. University of Calgary; Canadá
Fil: Bradley, Peter John. University of California at Los Angeles; Estados Unidos
Fil: Moore, Dadin Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Cobo, Eduardo Ruben. University of Calgary; Canadá
description Infections with the protozoan Neospora caninum cause abortion in cattle, likely due to the parasite’s replication and excessive inflammation in the placenta. Cathelicidins are host defense peptides known for their antimicrobial and immunomodulatory functions, but their role in N. caninum infections remains elusive. Using bone marrow-derived macrophages (BMDMs) isolated from mice expressing (wild-type, Camp+/+) and lacking (Camp−/−) cathelicidins, we investigated the role of endogenous cathelicidin in infections with N. caninum. We show that Camp−/− macrophages primed with lipopolysaccharide (LPS) had an increased number of intracellular N. caninum tachyzoites, and these macrophages released higher amounts of IL-1β and lactate dehydrogenase (LDH), a marker of cytotoxicity. These findings indicate that cathelicidins contribute to intracellular N. caninum control and inflammation by limiting the activation of the inflammasome, particularly under LPS-induced conditions. This insight reveals the immunomodulatory role of cathelicidins in controlling N. caninum-associated pathologies.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
2026-02-09T11:07:54Z
2026-02-09T11:07:54Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25115
https://www.mdpi.com/2076-0817/14/7/663
2076-0817
https://doi.org/10.3390/pathogens14070663
url http://hdl.handle.net/20.500.12123/25115
https://www.mdpi.com/2076-0817/14/7/663
https://doi.org/10.3390/pathogens14070663
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute, MDPI
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute, MDPI
dc.source.none.fl_str_mv Pathogens 14 (7) : 663 (July 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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