Immune response to Neospora caninum live tachyzoites in prepubertal female calves
- Autores
- Hecker, Yanina; Regidor-Cerrillo, Javier; Fiorani, Franco; Horcajo, Pilar; Soria, Ivana; Gual, Ignacio; Torioni, Susana Marta; Campero, Lucia; Echaide, Ignacio Eduardo; Alvarez-García, Gema; Ortega-Mora, Luis; Zamorano, Patricia Ines; Venturini, María Clara; Odeon, Anselmo Carlos; Canton, German Jose; Moore, Prando Dadin
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges.
EEA Balcarce
Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Regidor-Cerrillo, Javier. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Horcajo, Pilar. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.
Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.
Fil: Campero, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina.
Fil: Echaide, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.
Fil: Alvarez- García, Gema. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Ortega-Mora, Luis. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.
Fil: Zamorano, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Venturini, María Clara. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina.
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Moore, Dadín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina - Fuente
- Parasitollogy Research 18 (10) : 2945-2955. (October 2019)
- Materia
-
Neospora Caninum
Respuesta Inmunológica
Inoculación
Ternero
Inmune Response
Inoculation
Calves - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5993
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Immune response to Neospora caninum live tachyzoites in prepubertal female calvesHecker, YaninaRegidor-Cerrillo, JavierFiorani, FrancoHorcajo, PilarSoria, IvanaGual, IgnacioTorioni, Susana MartaCampero, LuciaEchaide, Ignacio EduardoAlvarez-García, GemaOrtega-Mora, LuisZamorano, Patricia InesVenturini, María ClaraOdeon, Anselmo CarlosCanton, German JoseMoore, Prando DadinNeospora CaninumRespuesta InmunológicaInoculaciónTerneroInmune ResponseInoculationCalvesThe aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges.EEA BalcarceFil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Regidor-Cerrillo, Javier. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Horcajo, Pilar. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.Fil: Campero, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina.Fil: Echaide, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina.Fil: Alvarez- García, Gema. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Ortega-Mora, Luis. Universidad de Madrid. Facultad de Ciencias Veterinarias; España.Fil: Zamorano, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Venturini, María Clara. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina.Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Moore, Dadín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaSpringer Berlin2019-09-27T10:55:06Z2019-09-27T10:55:06Z2019-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs00436-019-06447-yhttp://hdl.handle.net/20.500.12123/59930932-0113 (Print)1432-1955 (Online)https://doi.org/10.1007/s00436-019-06447-yParasitollogy Research 18 (10) : 2945-2955. 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| dc.title.none.fl_str_mv |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| spellingShingle |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves Hecker, Yanina Neospora Caninum Respuesta Inmunológica Inoculación Ternero Inmune Response Inoculation Calves |
| title_short |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_full |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_fullStr |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_full_unstemmed |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_sort |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| dc.creator.none.fl_str_mv |
Hecker, Yanina Regidor-Cerrillo, Javier Fiorani, Franco Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Marta Campero, Lucia Echaide, Ignacio Eduardo Alvarez-García, Gema Ortega-Mora, Luis Zamorano, Patricia Ines Venturini, María Clara Odeon, Anselmo Carlos Canton, German Jose Moore, Prando Dadin |
| author |
Hecker, Yanina |
| author_facet |
Hecker, Yanina Regidor-Cerrillo, Javier Fiorani, Franco Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Marta Campero, Lucia Echaide, Ignacio Eduardo Alvarez-García, Gema Ortega-Mora, Luis Zamorano, Patricia Ines Venturini, María Clara Odeon, Anselmo Carlos Canton, German Jose Moore, Prando Dadin |
| author_role |
author |
| author2 |
Regidor-Cerrillo, Javier Fiorani, Franco Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Marta Campero, Lucia Echaide, Ignacio Eduardo Alvarez-García, Gema Ortega-Mora, Luis Zamorano, Patricia Ines Venturini, María Clara Odeon, Anselmo Carlos Canton, German Jose Moore, Prando Dadin |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Neospora Caninum Respuesta Inmunológica Inoculación Ternero Inmune Response Inoculation Calves |
| topic |
Neospora Caninum Respuesta Inmunológica Inoculación Ternero Inmune Response Inoculation Calves |
| dc.description.none.fl_txt_mv |
The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges. EEA Balcarce Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Regidor-Cerrillo, Javier. Universidad de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Horcajo, Pilar. Universidad de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Torioni, Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Fil: Campero, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina. Fil: Echaide, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Fil: Alvarez- García, Gema. Universidad de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Ortega-Mora, Luis. Universidad de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Zamorano, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Venturini, María Clara. Universidad Nacional de La Plata, Facultad de Medicina Veterinaria; Argentina. Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Moore, Dadín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina |
| description |
The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019-09-27T10:55:06Z 2019-09-27T10:55:06Z 2019-09-04 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
| status_str |
publishedVersion |
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https://link.springer.com/article/10.1007%2Fs00436-019-06447-y http://hdl.handle.net/20.500.12123/5993 0932-0113 (Print) 1432-1955 (Online) https://doi.org/10.1007/s00436-019-06447-y |
| url |
https://link.springer.com/article/10.1007%2Fs00436-019-06447-y http://hdl.handle.net/20.500.12123/5993 https://doi.org/10.1007/s00436-019-06447-y |
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0932-0113 (Print) 1432-1955 (Online) |
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eng |
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eng |
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info:eu-repo/semantics/restrictedAccess |
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Springer Berlin |
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Springer Berlin |
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Parasitollogy Research 18 (10) : 2945-2955. (October 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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