Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation

Autores
Bartley, Paul M.; Katzer, Frank; Rocchi, Mara; Maley, Stephen W.; Benavides-Silván, Julio; Nath, Mintu; Pang, Yvonne; Canton, German Jose; Thomson, Jackie; Chianini, Francesca; Innes, Elisabeth A.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses.
EEA Balcarce
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unidos
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Thomson, Jackie. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fuente
Veterinary Research 44 : 91 (2013)
Materia
Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Respuesta Inmunológica
Feto
Cattle
Animal Diseases
Immune Response
Foetus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4000
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oai_identifier_str oai:localhost:20.500.12123/4000
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network_name_str INTA Digital (INTA)
spelling Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestationBartley, Paul M.Katzer, FrankRocchi, MaraMaley, Stephen W.Benavides-Silván, JulioNath, MintuPang, YvonneCanton, German JoseThomson, JackieChianini, FrancescaInnes, Elisabeth A.Ganado BovinoEnfermedades de los AnimalesNeospora caninumRespuesta InmunológicaFetoCattleAnimal DiseasesImmune ResponseFoetusThis study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses.EEA BalcarceFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Rocchi, Mara. Moredun Research Institute; Reino UnidoFil: Maley, Stephen W. Moredun Research Institute; Reino UnidoFil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; EspañaFil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino UnidosFil: Pang, Yvonne. Moredun Research Institute; Reino UnidoFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Thomson, Jackie. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute; Reino UnidoBMC2018-12-03T17:21:18Z2018-12-03T17:21:18Z2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91http://hdl.handle.net/20.500.12123/40000928-42491297-9716https://doi.org/10.1186/1297-9716-44-91Veterinary Research 44 : 91 (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:45Zoai:localhost:20.500.12123/4000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:45.675INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
title Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
spellingShingle Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
Bartley, Paul M.
Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Respuesta Inmunológica
Feto
Cattle
Animal Diseases
Immune Response
Foetus
title_short Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
title_full Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
title_fullStr Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
title_full_unstemmed Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
title_sort Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation
dc.creator.none.fl_str_mv Bartley, Paul M.
Katzer, Frank
Rocchi, Mara
Maley, Stephen W.
Benavides-Silván, Julio
Nath, Mintu
Pang, Yvonne
Canton, German Jose
Thomson, Jackie
Chianini, Francesca
Innes, Elisabeth A.
author Bartley, Paul M.
author_facet Bartley, Paul M.
Katzer, Frank
Rocchi, Mara
Maley, Stephen W.
Benavides-Silván, Julio
Nath, Mintu
Pang, Yvonne
Canton, German Jose
Thomson, Jackie
Chianini, Francesca
Innes, Elisabeth A.
author_role author
author2 Katzer, Frank
Rocchi, Mara
Maley, Stephen W.
Benavides-Silván, Julio
Nath, Mintu
Pang, Yvonne
Canton, German Jose
Thomson, Jackie
Chianini, Francesca
Innes, Elisabeth A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Respuesta Inmunológica
Feto
Cattle
Animal Diseases
Immune Response
Foetus
topic Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Respuesta Inmunológica
Feto
Cattle
Animal Diseases
Immune Response
Foetus
dc.description.none.fl_txt_mv This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses.
EEA Balcarce
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unidos
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Thomson, Jackie. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
description This study examined the immunological responses of pregnant cattle and their foetuses following an experimental challenge with live Neospora caninum tachyzoites at day 210 of gestation. Animals were bled prior to and weekly throughout the experiment and sacrificed at 14, 28, 42 and 56 days post inoculation (dpi). At post mortem examination, samples of lymph nodes and spleen were collected from both dam and foetus for immunological analysis. Subcutaneous (sc) inoculation over the left prefemoral (LPF) lymph node of pregnant cattle at day 210 of gestation, led to the vertical transmission of parasites by 14 dpi, however no foetal deaths were observed in the infected animals. Foetuses from infected dams mounted Neospora-specific humoral and cell-mediated immune (CMI) responses by 14 dpi. These responses involved anti-Neospora IgG, antigen-specific lymphocyte proliferation, and the production of the cytokines IFN–γ, interleukin (IL)-4 and IL-10. There was also evidence of innate immunity during the response against Neospora from infected dams, with statistically significant (p < 0.05) increases in mean expression of toll like receptors (TLR)-2 on 56 dpi in maternal spleen, LPF, right prefemoral (RPF), left uterine (LUL) and right uterine (RUL) lymph nodes and TLR-9 in retropharyngeal (RLN), LPF and RPF lymph nodes from 28 dpi. Statistically significant (p < 0.05) increases in mean TLR-9 were detected in spleen samples from foetuses of infected dams, compared to the foetuses from control animals. Our results show that vertical transmission of the parasite occurred in all infected dams, with their foetuses showing effective Neospora-specific cell mediated, humoral and innate immune responses.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2018-12-03T17:21:18Z
2018-12-03T17:21:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91
http://hdl.handle.net/20.500.12123/4000
0928-4249
1297-9716
https://doi.org/10.1186/1297-9716-44-91
url https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-91
http://hdl.handle.net/20.500.12123/4000
https://doi.org/10.1186/1297-9716-44-91
identifier_str_mv 0928-4249
1297-9716
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Veterinary Research 44 : 91 (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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