Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes

Autores
Soto, Gabriela Cynthia; Fox, Ana Romina; Ayub, Nicolás Daniel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes.
Instituto de Genética
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Molecular Evolution 77 (1–2) : 3–7 (August 2013)
Materia
Fijación Biológica del Nitrógeno
Eukaryota
Oxígeno
Biological Nitrogen Fixation
Oxygen
Eukaryotes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4296
Acceder
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spelling Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to EukaryotesSoto, Gabriela CynthiaFox, Ana RominaAyub, Nicolás DanielFijación Biológica del NitrógenoEukaryotaOxígenoBiological Nitrogen FixationOxygenEukaryotesBiological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes.Instituto de GenéticaFil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2019-01-18T14:53:36Z2019-01-18T14:53:36Z2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00239-013-9578-8http://hdl.handle.net/20.500.12123/42960022-28441432-1432https://doi.org/10.1007/s00239-013-9578-8Journal of Molecular Evolution 77 (1–2) : 3–7 (August 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4296instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:33.518INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
title Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
spellingShingle Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
Soto, Gabriela Cynthia
Fijación Biológica del Nitrógeno
Eukaryota
Oxígeno
Biological Nitrogen Fixation
Oxygen
Eukaryotes
title_short Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
title_full Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
title_fullStr Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
title_full_unstemmed Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
title_sort Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes
dc.creator.none.fl_str_mv Soto, Gabriela Cynthia
Fox, Ana Romina
Ayub, Nicolás Daniel
author Soto, Gabriela Cynthia
author_facet Soto, Gabriela Cynthia
Fox, Ana Romina
Ayub, Nicolás Daniel
author_role author
author2 Fox, Ana Romina
Ayub, Nicolás Daniel
author2_role author
author
dc.subject.none.fl_str_mv Fijación Biológica del Nitrógeno
Eukaryota
Oxígeno
Biological Nitrogen Fixation
Oxygen
Eukaryotes
topic Fijación Biológica del Nitrógeno
Eukaryota
Oxígeno
Biological Nitrogen Fixation
Oxygen
Eukaryotes
dc.description.none.fl_txt_mv Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes.
Instituto de Genética
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fox, Ana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Biological nitrogen fixation is widespread among the Eubacteria and Archae domains but completely absent in eukaryotes. The lack of lateral transfer of nitrogen-fixation genes from prokaryotes to eukaryotes has been partially attributed to the physiological requirements necessary for the function of the nitrogenase complex. However, symbiotic bacterial nitrogenase activity is protected by the nodule, a plant structure whose organogenesis can be trigged in the absence of bacteria. To explore the intrinsic potentiality of this plant organ, we generated rhizobium-independent nodules in alfalfa by overexpressing the MsDMI3 kinase lacking the autoinhibitory domain. These transgenic nodules showed similar levels of leghemoglobin, free oxygen, ATP, and NADPH to those of efficient Sinorhizobium meliloti B399-infected nodules, suggesting that the rhizobium-independent nodules can provide an optimal microenvironment for nitrogenase activity. Finally, we discuss the intrinsic evolutionary constraints on transfer of nitrogen-fixation genes between bacteria and eukaryotes.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
2019-01-18T14:53:36Z
2019-01-18T14:53:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00239-013-9578-8
http://hdl.handle.net/20.500.12123/4296
0022-2844
1432-1432
https://doi.org/10.1007/s00239-013-9578-8
url https://link.springer.com/article/10.1007/s00239-013-9578-8
http://hdl.handle.net/20.500.12123/4296
https://doi.org/10.1007/s00239-013-9578-8
identifier_str_mv 0022-2844
1432-1432
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Molecular Evolution 77 (1–2) : 3–7 (August 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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