The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components

Autores
Gao, Haixiao; Ayub, Maximiliano Juri; Levin, Mariano Jorge; Frank, Joachim
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes.
Fil: Gao, Haixiao. Howard Hughes Medical Institute; Estados Unidos
Fil: Ayub, Maximiliano Juri. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Frank, Joachim. State University of New York; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Materia
Eukaryote
Initiation
Translation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39400
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA componentsGao, HaixiaoAyub, Maximiliano JuriLevin, Mariano JorgeFrank, JoachimEukaryoteInitiationTranslationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes.Fil: Gao, Haixiao. Howard Hughes Medical Institute; Estados UnidosFil: Ayub, Maximiliano Juri. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Frank, Joachim. State University of New York; Estados Unidos. Howard Hughes Medical Institute; Estados UnidosNational Academy of Sciences2005-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39400Gao, Haixiao; Ayub, Maximiliano Juri; Levin, Mariano Jorge; Frank, Joachim; The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 102; 29; 7-2005; 10206-102110027-84241091-6490CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/102/29/10206.longinfo:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0500926102info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:39Zoai:ri.conicet.gov.ar:11336/39400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:39.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
title The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
spellingShingle The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
Gao, Haixiao
Eukaryote
Initiation
Translation
title_short The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
title_full The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
title_fullStr The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
title_full_unstemmed The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
title_sort The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components
dc.creator.none.fl_str_mv Gao, Haixiao
Ayub, Maximiliano Juri
Levin, Mariano Jorge
Frank, Joachim
author Gao, Haixiao
author_facet Gao, Haixiao
Ayub, Maximiliano Juri
Levin, Mariano Jorge
Frank, Joachim
author_role author
author2 Ayub, Maximiliano Juri
Levin, Mariano Jorge
Frank, Joachim
author2_role author
author
author
dc.subject.none.fl_str_mv Eukaryote
Initiation
Translation
topic Eukaryote
Initiation
Translation
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes.
Fil: Gao, Haixiao. Howard Hughes Medical Institute; Estados Unidos
Fil: Ayub, Maximiliano Juri. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Frank, Joachim. State University of New York; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
description We present analysis, by cryo-electron microscopy and single-particle reconstruction, of the structure of the 80S ribosome from Trypanosoma cruzi, the kinetoplastid protozoan pathogen that causes Chagas disease. The density map of the T. cruzi 80S ribosome shows the phylogenetically conserved eukaryotic rRNA core structure, together with distinctive structural features in both the small and large subunits. Remarkably, a previously undescribed helical structure appears in the small subunit in the vicinity of the mRNA exit channel. We propose that this rRNA structure likely participates in the recruitment of ribosome onto the 5′ end of mRNA, in facilitating and modulating the initiation of translation that is unique to the trypanosomes.
publishDate 2005
dc.date.none.fl_str_mv 2005-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39400
Gao, Haixiao; Ayub, Maximiliano Juri; Levin, Mariano Jorge; Frank, Joachim; The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 102; 29; 7-2005; 10206-10211
0027-8424
1091-6490
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39400
identifier_str_mv Gao, Haixiao; Ayub, Maximiliano Juri; Levin, Mariano Jorge; Frank, Joachim; The structure of the 80S ribosome from Trypanosoma cruzi reveals unique rRNA components; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 102; 29; 7-2005; 10206-10211
0027-8424
1091-6490
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.pnas.org/content/102/29/10206.long
info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.0500926102
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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