Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobiu...

Autores: Muñoz, Nacira Belen; Robert, German; Melchiorre, Mariana; Racca, Roberto Walter; Lascano, Hernan Ramiro
Año de publicación: 2011
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150 mM NaCl. In inoculated root hairs under 50 mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150 mM NaCl in both inoculated and noninoculated roots and by 50 mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fuente: Environmental and Experimental Botany 78 : 76-83 (May 2012)
Materia: Salinity
Legumes
Osmotic Stress
Soybeans
Reactive Oxygen Species
Salinidad
Leguminosas
Estrés Osmótico
Rhizobium
Bradyrhizobium japonicum
Soja
Especies de Oxígeno Reactivo
Root Hair Deformation
ROS Production
Root Hair Death
Legume Interaction
Estrés Salino
Nivel de accesibilidad: acceso restringido
Condiciones de uso
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/10983

Acceder

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network_name_str	INTA Digital (INTA)
spelling	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interactionMuñoz, Nacira BelenRobert, GermanMelchiorre, MarianaRacca, Roberto WalterLascano, Hernan RamiroSalinityLegumesOsmotic StressSoybeansReactive Oxygen SpeciesSalinidadLeguminosasEstrés OsmóticoRhizobiumBradyrhizobium japonicumSojaEspecies de Oxígeno ReactivoRoot Hair DeformationROS ProductionRoot Hair DeathLegume InteractionEstrés SalinoIn the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150 mM NaCl. In inoculated root hairs under 50 mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150 mM NaCl in both inoculated and noninoculated roots and by 50 mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaElsevier2021-12-23T13:25:42Z2021-12-23T13:25:42Z2011-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10983https://www.sciencedirect.com/science/article/pii/S00988472110031570098-8472https://doi.org/10.1016/j.envexpbot.2011.12.008Environmental and Experimental Botany 78 : 76-83 (May 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:26Zoai:localhost:20.500.12123/10983instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:26.884INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
title	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
spellingShingle	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction Muñoz, Nacira Belen Salinity Legumes Osmotic Stress Soybeans Reactive Oxygen Species Salinidad Leguminosas Estrés Osmótico Rhizobium Bradyrhizobium japonicum Soja Especies de Oxígeno Reactivo Root Hair Deformation ROS Production Root Hair Death Legume Interaction Estrés Salino
title_short	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
title_full	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
title_fullStr	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
title_full_unstemmed	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
title_sort	Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobium japonicum interaction
dc.creator.none.fl_str_mv	Muñoz, Nacira Belen Robert, German Melchiorre, Mariana Racca, Roberto Walter Lascano, Hernan Ramiro
author	Muñoz, Nacira Belen
author_facet	Muñoz, Nacira Belen Robert, German Melchiorre, Mariana Racca, Roberto Walter Lascano, Hernan Ramiro
author_role	author
author2	Robert, German Melchiorre, Mariana Racca, Roberto Walter Lascano, Hernan Ramiro
author2_role	author author author author
dc.subject.none.fl_str_mv	Salinity Legumes Osmotic Stress Soybeans Reactive Oxygen Species Salinidad Leguminosas Estrés Osmótico Rhizobium Bradyrhizobium japonicum Soja Especies de Oxígeno Reactivo Root Hair Deformation ROS Production Root Hair Death Legume Interaction Estrés Salino
topic	Salinity Legumes Osmotic Stress Soybeans Reactive Oxygen Species Salinidad Leguminosas Estrés Osmótico Rhizobium Bradyrhizobium japonicum Soja Especies de Oxígeno Reactivo Root Hair Deformation ROS Production Root Hair Death Legume Interaction Estrés Salino
dc.description.none.fl_txt_mv	In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150 mM NaCl. In inoculated root hairs under 50 mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150 mM NaCl in both inoculated and noninoculated roots and by 50 mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Muñoz, Nacira Belen. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Robert, German. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
description	In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150 mM NaCl. In inoculated root hairs under 50 mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150 mM NaCl in both inoculated and noninoculated roots and by 50 mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.
publishDate	2011
dc.date.none.fl_str_mv	2011-12-08 2021-12-23T13:25:42Z 2021-12-23T13:25:42Z
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/10983 https://www.sciencedirect.com/science/article/pii/S0098847211003157 0098-8472 https://doi.org/10.1016/j.envexpbot.2011.12.008
url	http://hdl.handle.net/20.500.12123/10983 https://www.sciencedirect.com/science/article/pii/S0098847211003157 https://doi.org/10.1016/j.envexpbot.2011.12.008
identifier_str_mv	0098-8472
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv	restrictedAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	Environmental and Experimental Botany 78 : 76-83 (May 2012) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
collection	INTA Digital (INTA)
instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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score	12.559606

Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.–Bradyrhizobiu...

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