Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobiu...

Autores
Muñoz, Nacira Belén; Robert, Germán; Melchiorre, Mariana Noemi; Racca, Roberto Walter; Lascano, Hernan Ramiro
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150. mM NaCl. In inoculated root hairs under 50. mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150. mM NaCl in both inoculated and noninoculated roots and by 50. mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana Noemi. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
LEGUME INTERACTION
RHIZOBIUM
ROOT HAIR DEATH
ROOT HAIR DEFORMATION
ROS PRODUCTION
SALINITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199034
Acceder
id CONICETDig_53c4c62035a7c37ee59715a918033c14
oai_identifier_str oai:ri.conicet.gov.ar:11336/199034
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interactionMuñoz, Nacira BelénRobert, GermánMelchiorre, Mariana NoemiRacca, Roberto WalterLascano, Hernan RamiroLEGUME INTERACTIONRHIZOBIUMROOT HAIR DEATHROOT HAIR DEFORMATIONROS PRODUCTIONSALINITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150. mM NaCl. In inoculated root hairs under 50. mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150. mM NaCl in both inoculated and noninoculated roots and by 50. mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Melchiorre, Mariana Noemi. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaPergamon-Elsevier Science Ltd2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199034Muñoz, Nacira Belén; Robert, Germán; Melchiorre, Mariana Noemi; Racca, Roberto Walter; Lascano, Hernan Ramiro; Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 78; 5-2012; 76-830098-8472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847211003157info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2011.12.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:19:22Zoai:ri.conicet.gov.ar:11336/199034instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:19:22.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
title Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
spellingShingle Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
Muñoz, Nacira Belén
LEGUME INTERACTION
RHIZOBIUM
ROOT HAIR DEATH
ROOT HAIR DEFORMATION
ROS PRODUCTION
SALINITY
title_short Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
title_full Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
title_fullStr Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
title_full_unstemmed Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
title_sort Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction
dc.creator.none.fl_str_mv Muñoz, Nacira Belén
Robert, Germán
Melchiorre, Mariana Noemi
Racca, Roberto Walter
Lascano, Hernan Ramiro
author Muñoz, Nacira Belén
author_facet Muñoz, Nacira Belén
Robert, Germán
Melchiorre, Mariana Noemi
Racca, Roberto Walter
Lascano, Hernan Ramiro
author_role author
author2 Robert, Germán
Melchiorre, Mariana Noemi
Racca, Roberto Walter
Lascano, Hernan Ramiro
author2_role author
author
author
author
dc.subject.none.fl_str_mv LEGUME INTERACTION
RHIZOBIUM
ROOT HAIR DEATH
ROOT HAIR DEFORMATION
ROS PRODUCTION
SALINITY
topic LEGUME INTERACTION
RHIZOBIUM
ROOT HAIR DEATH
ROOT HAIR DEFORMATION
ROS PRODUCTION
SALINITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150. mM NaCl. In inoculated root hairs under 50. mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150. mM NaCl in both inoculated and noninoculated roots and by 50. mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Melchiorre, Mariana Noemi. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description In the present study, the production of apoplastic and intracellular reactive oxygen species (ROS) and deformations of young soybean root hairs inoculated with Bradyrhizobium japonicum strain USDA138 were analyzed under saline and osmotic stress. Sustained and transient increase of apoplastic and intracellular ROS production, respectively, were observed in inoculated root hairs. The apical production of apoplastic superoxide in growing root hairs colocalized with flavonoid autofluorescence and both were relocated to the zone of maximum curvature in curled root hairs. Saline and osmotic stress had differential effects on both the production of apoplastic ROS and curling: only saline stress inhibited both processes in a dose-dependent manner. Intracellular ROS production was not altered by osmotic stress but was inhibited completely by 150. mM NaCl. In inoculated root hairs under 50. mM NaCl, the intracellular ROS levels were initially increased, but not decreased at later stages, as occurred in control conditions. Root hair death was induced by 150. mM NaCl in both inoculated and noninoculated roots and by 50. mM NaCl only in inoculated roots. Saline, but not osmotic stress, marked affects both apoplastic and intracellular ROS production, inhibiting root hair curling and inducing root hair death.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199034
Muñoz, Nacira Belén; Robert, Germán; Melchiorre, Mariana Noemi; Racca, Roberto Walter; Lascano, Hernan Ramiro; Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 78; 5-2012; 76-83
0098-8472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199034
identifier_str_mv Muñoz, Nacira Belén; Robert, Germán; Melchiorre, Mariana Noemi; Racca, Roberto Walter; Lascano, Hernan Ramiro; Saline and osmotic stress differentially affects apoplastic and intracellular reactive oxygen species production, curling and death of root hair during Glycine max L.-Bradyrhizobium japonicum interaction; Pergamon-Elsevier Science Ltd; Environmental and Experimental Botany; 78; 5-2012; 76-83
0098-8472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0098847211003157
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envexpbot.2011.12.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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