Interaction of Nisin with Anionic Membranes: Purification and Raman Study

Autores
Sosa Morales, Marcelo Clemente; Bellomio, Augusto; Juárez, Ana Carolina; Alvarez, Rosa Maria Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Nisin is an antibiotic peptide ribosomally sintetized by Lactococcus lactis that inhibits the growth of a wide range of gram-positive bacteria. It has a net positive charge (+5) and its weight is approximately 3kDa. Commercially, it is available as a lyophilized powder containing 2.5% (w/w) nisin. Purification was carried out using chromatographic methods. The bactericidal activity of fractions obtained was visualized on agar plates. In addition, Tricine SDS-Page and Mass Spectrometry were performed to confirm the purity of nisin. Raman Spectroscopy was used to study the interaction of this small peptide with anionic models membranes. We evaluated and interpretated signal spectral changes considered specific markers of the lipid packaging, the number of conformers gauche in the hydrocarbon chains and the degree of hydration of the polar heads. For this purpose, dipalmitoylphosphatidylglycerol (DPPG) and dilauroylphosphatidylglycerol (DLPG) were utilized. Measurements were made at room temperature. Analysis of DPPG/Nisin and DLPG/Nisin systems will allow us to know if lipid phase state impact on the interaction and hence to have a better understanding about the action mechanism of nisin.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Juárez, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
XLII Reunión Anual de la Sociedad Argentina de Biofísica
Villa Carlos Paz
Argentina
Sociedad Argentina de Biofísica
Materia
NISIN
RAMAN
ANIONIC MEMBRANES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258084
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/258084
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interaction of Nisin with Anionic Membranes: Purification and Raman StudySosa Morales, Marcelo ClementeBellomio, AugustoJuárez, Ana CarolinaAlvarez, Rosa Maria SusanaNISINRAMANANIONIC MEMBRANEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Nisin is an antibiotic peptide ribosomally sintetized by Lactococcus lactis that inhibits the growth of a wide range of gram-positive bacteria. It has a net positive charge (+5) and its weight is approximately 3kDa. Commercially, it is available as a lyophilized powder containing 2.5% (w/w) nisin. Purification was carried out using chromatographic methods. The bactericidal activity of fractions obtained was visualized on agar plates. In addition, Tricine SDS-Page and Mass Spectrometry were performed to confirm the purity of nisin. Raman Spectroscopy was used to study the interaction of this small peptide with anionic models membranes. We evaluated and interpretated signal spectral changes considered specific markers of the lipid packaging, the number of conformers gauche in the hydrocarbon chains and the degree of hydration of the polar heads. For this purpose, dipalmitoylphosphatidylglycerol (DPPG) and dilauroylphosphatidylglycerol (DLPG) were utilized. Measurements were made at room temperature. Analysis of DPPG/Nisin and DLPG/Nisin systems will allow us to know if lipid phase state impact on the interaction and hence to have a better understanding about the action mechanism of nisin.Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Juárez, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; ArgentinaFil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaXLII Reunión Anual de la Sociedad Argentina de BiofísicaVilla Carlos PazArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/258084Interaction of Nisin with Anionic Membranes: Purification and Raman Study; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Villa Carlos Paz; Argentina; 2013; 38-38978-987-27591-2-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:43Zoai:ri.conicet.gov.ar:11336/258084instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:43.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction of Nisin with Anionic Membranes: Purification and Raman Study
title Interaction of Nisin with Anionic Membranes: Purification and Raman Study
spellingShingle Interaction of Nisin with Anionic Membranes: Purification and Raman Study
Sosa Morales, Marcelo Clemente
NISIN
RAMAN
ANIONIC MEMBRANES
title_short Interaction of Nisin with Anionic Membranes: Purification and Raman Study
title_full Interaction of Nisin with Anionic Membranes: Purification and Raman Study
title_fullStr Interaction of Nisin with Anionic Membranes: Purification and Raman Study
title_full_unstemmed Interaction of Nisin with Anionic Membranes: Purification and Raman Study
title_sort Interaction of Nisin with Anionic Membranes: Purification and Raman Study
dc.creator.none.fl_str_mv Sosa Morales, Marcelo Clemente
Bellomio, Augusto
Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author Sosa Morales, Marcelo Clemente
author_facet Sosa Morales, Marcelo Clemente
Bellomio, Augusto
Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author_role author
author2 Bellomio, Augusto
Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author2_role author
author
author
dc.subject.none.fl_str_mv NISIN
RAMAN
ANIONIC MEMBRANES
topic NISIN
RAMAN
ANIONIC MEMBRANES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nisin is an antibiotic peptide ribosomally sintetized by Lactococcus lactis that inhibits the growth of a wide range of gram-positive bacteria. It has a net positive charge (+5) and its weight is approximately 3kDa. Commercially, it is available as a lyophilized powder containing 2.5% (w/w) nisin. Purification was carried out using chromatographic methods. The bactericidal activity of fractions obtained was visualized on agar plates. In addition, Tricine SDS-Page and Mass Spectrometry were performed to confirm the purity of nisin. Raman Spectroscopy was used to study the interaction of this small peptide with anionic models membranes. We evaluated and interpretated signal spectral changes considered specific markers of the lipid packaging, the number of conformers gauche in the hydrocarbon chains and the degree of hydration of the polar heads. For this purpose, dipalmitoylphosphatidylglycerol (DPPG) and dilauroylphosphatidylglycerol (DLPG) were utilized. Measurements were made at room temperature. Analysis of DPPG/Nisin and DLPG/Nisin systems will allow us to know if lipid phase state impact on the interaction and hence to have a better understanding about the action mechanism of nisin.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bellomio, Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Juárez, Ana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Física; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
XLII Reunión Anual de la Sociedad Argentina de Biofísica
Villa Carlos Paz
Argentina
Sociedad Argentina de Biofísica
description Nisin is an antibiotic peptide ribosomally sintetized by Lactococcus lactis that inhibits the growth of a wide range of gram-positive bacteria. It has a net positive charge (+5) and its weight is approximately 3kDa. Commercially, it is available as a lyophilized powder containing 2.5% (w/w) nisin. Purification was carried out using chromatographic methods. The bactericidal activity of fractions obtained was visualized on agar plates. In addition, Tricine SDS-Page and Mass Spectrometry were performed to confirm the purity of nisin. Raman Spectroscopy was used to study the interaction of this small peptide with anionic models membranes. We evaluated and interpretated signal spectral changes considered specific markers of the lipid packaging, the number of conformers gauche in the hydrocarbon chains and the degree of hydration of the polar heads. For this purpose, dipalmitoylphosphatidylglycerol (DPPG) and dilauroylphosphatidylglycerol (DLPG) were utilized. Measurements were made at room temperature. Analysis of DPPG/Nisin and DLPG/Nisin systems will allow us to know if lipid phase state impact on the interaction and hence to have a better understanding about the action mechanism of nisin.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258084
Interaction of Nisin with Anionic Membranes: Purification and Raman Study; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Villa Carlos Paz; Argentina; 2013; 38-38
978-987-27591-2-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258084
identifier_str_mv Interaction of Nisin with Anionic Membranes: Purification and Raman Study; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Villa Carlos Paz; Argentina; 2013; 38-38
978-987-27591-2-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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