Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers

Autores
Sosa Morales, Marcelo Clemente; Juárez, Ana Carolina; Alvarez, Rosa Maria Susana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Raman spectroscopic study of anionic model membranes and their structural alterations exerted by a relatively small biomolecule, such as the hormone 3,5,3′-triiodo-L-thyronine (T3), is presented. Spectral differences between pure dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles and DPPG?T3 mixture and between pure dilauroylphosphatidylglycerol (DLPG) and DLPG?T3 mixture were evaluated in order to determine the response of lipid membranes in gel and liquid-crystalline phases to the hormone incorporation. Density functional theory (DFT) calculations support the band analysis of the complex 1150-1050 cm−1 Raman region. Geometry optimizations and vibrational behaviors of a model charged molecule that mimics the phosphatidylglycerol lipid moiety in solvated state were taken into account for the spectral interpretation of this specific region. The anionic nature of the lipid polar head plays an important role in the interaction with the hormone, as is evidenced by the C[DOUBLE BOND]O and PO2− stretching bands. In addition, the differential penetration of T3 into the hydrophobic region of the membranes shows to be dependent on the lipid phase. The spectral data were compared with those previously obtained for zwitterionic membranes.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Juárez, Ana Carolina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; Argentina
Materia
Phospholipids
Anionic Membranes
Vibrational Analysis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6687
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6687
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayersSosa Morales, Marcelo ClementeJuárez, Ana CarolinaAlvarez, Rosa Maria SusanaPhospholipidsAnionic MembranesVibrational Analysishttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A Raman spectroscopic study of anionic model membranes and their structural alterations exerted by a relatively small biomolecule, such as the hormone 3,5,3′-triiodo-L-thyronine (T3), is presented. Spectral differences between pure dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles and DPPG?T3 mixture and between pure dilauroylphosphatidylglycerol (DLPG) and DLPG?T3 mixture were evaluated in order to determine the response of lipid membranes in gel and liquid-crystalline phases to the hormone incorporation. Density functional theory (DFT) calculations support the band analysis of the complex 1150-1050 cm−1 Raman region. Geometry optimizations and vibrational behaviors of a model charged molecule that mimics the phosphatidylglycerol lipid moiety in solvated state were taken into account for the spectral interpretation of this specific region. The anionic nature of the lipid polar head plays an important role in the interaction with the hormone, as is evidenced by the C[DOUBLE BOND]O and PO2− stretching bands. In addition, the differential penetration of T3 into the hydrophobic region of the membranes shows to be dependent on the lipid phase. The spectral data were compared with those previously obtained for zwitterionic membranes.Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán; ArgentinaFil: Juárez, Ana Carolina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; ArgentinaJohn Wiley & Sons Ltd2015-01-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6687Sosa Morales, Marcelo Clemente; Juárez, Ana Carolina; Alvarez, Rosa Maria Susana; Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers; John Wiley & Sons Ltd; Journal Of Raman Spectroscopy; 46; 3; 15-1-2015; 302-3080377-0486enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1002/jrs.4639info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jrs.4639/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:43Zoai:ri.conicet.gov.ar:11336/6687instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:43.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
title Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
spellingShingle Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
Sosa Morales, Marcelo Clemente
Phospholipids
Anionic Membranes
Vibrational Analysis
title_short Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
title_full Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
title_fullStr Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
title_full_unstemmed Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
title_sort Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers
dc.creator.none.fl_str_mv Sosa Morales, Marcelo Clemente
Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author Sosa Morales, Marcelo Clemente
author_facet Sosa Morales, Marcelo Clemente
Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author_role author
author2 Juárez, Ana Carolina
Alvarez, Rosa Maria Susana
author2_role author
author
dc.subject.none.fl_str_mv Phospholipids
Anionic Membranes
Vibrational Analysis
topic Phospholipids
Anionic Membranes
Vibrational Analysis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A Raman spectroscopic study of anionic model membranes and their structural alterations exerted by a relatively small biomolecule, such as the hormone 3,5,3′-triiodo-L-thyronine (T3), is presented. Spectral differences between pure dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles and DPPG?T3 mixture and between pure dilauroylphosphatidylglycerol (DLPG) and DLPG?T3 mixture were evaluated in order to determine the response of lipid membranes in gel and liquid-crystalline phases to the hormone incorporation. Density functional theory (DFT) calculations support the band analysis of the complex 1150-1050 cm−1 Raman region. Geometry optimizations and vibrational behaviors of a model charged molecule that mimics the phosphatidylglycerol lipid moiety in solvated state were taken into account for the spectral interpretation of this specific region. The anionic nature of the lipid polar head plays an important role in the interaction with the hormone, as is evidenced by the C[DOUBLE BOND]O and PO2− stretching bands. In addition, the differential penetration of T3 into the hydrophobic region of the membranes shows to be dependent on the lipid phase. The spectral data were compared with those previously obtained for zwitterionic membranes.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán; Argentina
Fil: Juárez, Ana Carolina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucuman. Facultad de Bioquimica, Quimica y Farmacia. Instituto de Quimica Fisica; Argentina
description A Raman spectroscopic study of anionic model membranes and their structural alterations exerted by a relatively small biomolecule, such as the hormone 3,5,3′-triiodo-L-thyronine (T3), is presented. Spectral differences between pure dipalmitoylphosphatidylglycerol (DPPG) multilamellar vesicles and DPPG?T3 mixture and between pure dilauroylphosphatidylglycerol (DLPG) and DLPG?T3 mixture were evaluated in order to determine the response of lipid membranes in gel and liquid-crystalline phases to the hormone incorporation. Density functional theory (DFT) calculations support the band analysis of the complex 1150-1050 cm−1 Raman region. Geometry optimizations and vibrational behaviors of a model charged molecule that mimics the phosphatidylglycerol lipid moiety in solvated state were taken into account for the spectral interpretation of this specific region. The anionic nature of the lipid polar head plays an important role in the interaction with the hormone, as is evidenced by the C[DOUBLE BOND]O and PO2− stretching bands. In addition, the differential penetration of T3 into the hydrophobic region of the membranes shows to be dependent on the lipid phase. The spectral data were compared with those previously obtained for zwitterionic membranes.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6687
Sosa Morales, Marcelo Clemente; Juárez, Ana Carolina; Alvarez, Rosa Maria Susana; Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers; John Wiley & Sons Ltd; Journal Of Raman Spectroscopy; 46; 3; 15-1-2015; 302-308
0377-0486
url http://hdl.handle.net/11336/6687
identifier_str_mv Sosa Morales, Marcelo Clemente; Juárez, Ana Carolina; Alvarez, Rosa Maria Susana; Raman spectroscopy study of the interaction of 3,5,3 ′ -triiodo-L-thyronine with phosphatidylglycerol lipid bilayers; John Wiley & Sons Ltd; Journal Of Raman Spectroscopy; 46; 3; 15-1-2015; 302-308
0377-0486
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1002/jrs.4639
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jrs.4639/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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