Patrón de glicosilación glandular y vascular de la placenta porcina

Autores
Sanchis, Eva Gabriela; Cristofolini, Andrea Lorena; Chanique, Analia Maria Luisa; Merkis, Cecilia Inés
Año de publicación
2012
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Since porcine placenta is epitheliochorial and non invasive, maternal and fetal blood flows are separated by six tissue layers. Therefore, the interchange of nutrients between mother and fetuses results indispensable for the success of pregnancy. The glycoconjugates participate in several biological processes, nevertheless their involvement in the materno-fetal interchange that takes place in the placenta is still unclear. The objective was to investigate the glycosilated residues of endometrial glands and blood vessels in placentas of different gestational periods. Crossbred swines from slaughterhouses located in Río Cuarto, Argentina, were used. Samples from placentas of: 28 (n=5), 55 (n=5), 60 (n=5), 70 (n=5) and 114 days of pregnancy (n=5) were obtained. Lectin-histochemistry using fluorescein isothiocyanate-conjugated lectins were employed. Sialic acid residues were detected with low intensity in blood vessels and uterine glands. Variable intensity was found for galactosilated residues, with the lowest intensity for ß-gal (1,3) galNAc residues of PNA binding. However, blood vessels staining was higher than that of glands. The highest staining for glucose and mannose was found in vessels with Con-A lectin, and in glands with VFA. The present results indicate that changes in the glycosilation pattern of glandular and vascular epithelia are related with placental remodeling along gestation. Moreover, the saccharide residues found in vascular and glandular secretions would be part of the nutritional substances provided to the embryos/fetuses during pregnancy.
Fil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Chanique, Analia Maria Luisa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina
Materia
PLACENTA
GESTATIONAL PERIODS
LECTINS
GLICOCONJUGATES
PORCINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195839
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Patrón de glicosilación glandular y vascular de la placenta porcinaSanchis, Eva GabrielaCristofolini, Andrea LorenaChanique, Analia Maria LuisaMerkis, Cecilia InésPLACENTAGESTATIONAL PERIODSLECTINSGLICOCONJUGATESPORCINEShttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Since porcine placenta is epitheliochorial and non invasive, maternal and fetal blood flows are separated by six tissue layers. Therefore, the interchange of nutrients between mother and fetuses results indispensable for the success of pregnancy. The glycoconjugates participate in several biological processes, nevertheless their involvement in the materno-fetal interchange that takes place in the placenta is still unclear. The objective was to investigate the glycosilated residues of endometrial glands and blood vessels in placentas of different gestational periods. Crossbred swines from slaughterhouses located in Río Cuarto, Argentina, were used. Samples from placentas of: 28 (n=5), 55 (n=5), 60 (n=5), 70 (n=5) and 114 days of pregnancy (n=5) were obtained. Lectin-histochemistry using fluorescein isothiocyanate-conjugated lectins were employed. Sialic acid residues were detected with low intensity in blood vessels and uterine glands. Variable intensity was found for galactosilated residues, with the lowest intensity for ß-gal (1,3) galNAc residues of PNA binding. However, blood vessels staining was higher than that of glands. The highest staining for glucose and mannose was found in vessels with Con-A lectin, and in glands with VFA. The present results indicate that changes in the glycosilation pattern of glandular and vascular epithelia are related with placental remodeling along gestation. Moreover, the saccharide residues found in vascular and glandular secretions would be part of the nutritional substances provided to the embryos/fetuses during pregnancy.Fil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Chanique, Analia Maria Luisa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; ArgentinaFil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; ArgentinaVeterinaria Organización2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195839Sanchis, Eva Gabriela; Cristofolini, Andrea Lorena; Chanique, Analia Maria Luisa; Merkis, Cecilia Inés; Patrón de glicosilación glandular y vascular de la placenta porcina; Veterinaria Organización; Redvet; 13; 3; 3-2012; 1-151695-7504CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.redalyc.org/articulo.oa?id=63623410007info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:33Zoai:ri.conicet.gov.ar:11336/195839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:33.397CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Patrón de glicosilación glandular y vascular de la placenta porcina
title Patrón de glicosilación glandular y vascular de la placenta porcina
spellingShingle Patrón de glicosilación glandular y vascular de la placenta porcina
Sanchis, Eva Gabriela
PLACENTA
GESTATIONAL PERIODS
LECTINS
GLICOCONJUGATES
PORCINES
title_short Patrón de glicosilación glandular y vascular de la placenta porcina
title_full Patrón de glicosilación glandular y vascular de la placenta porcina
title_fullStr Patrón de glicosilación glandular y vascular de la placenta porcina
title_full_unstemmed Patrón de glicosilación glandular y vascular de la placenta porcina
title_sort Patrón de glicosilación glandular y vascular de la placenta porcina
dc.creator.none.fl_str_mv Sanchis, Eva Gabriela
Cristofolini, Andrea Lorena
Chanique, Analia Maria Luisa
Merkis, Cecilia Inés
author Sanchis, Eva Gabriela
author_facet Sanchis, Eva Gabriela
Cristofolini, Andrea Lorena
Chanique, Analia Maria Luisa
Merkis, Cecilia Inés
author_role author
author2 Cristofolini, Andrea Lorena
Chanique, Analia Maria Luisa
Merkis, Cecilia Inés
author2_role author
author
author
dc.subject.none.fl_str_mv PLACENTA
GESTATIONAL PERIODS
LECTINS
GLICOCONJUGATES
PORCINES
topic PLACENTA
GESTATIONAL PERIODS
LECTINS
GLICOCONJUGATES
PORCINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Since porcine placenta is epitheliochorial and non invasive, maternal and fetal blood flows are separated by six tissue layers. Therefore, the interchange of nutrients between mother and fetuses results indispensable for the success of pregnancy. The glycoconjugates participate in several biological processes, nevertheless their involvement in the materno-fetal interchange that takes place in the placenta is still unclear. The objective was to investigate the glycosilated residues of endometrial glands and blood vessels in placentas of different gestational periods. Crossbred swines from slaughterhouses located in Río Cuarto, Argentina, were used. Samples from placentas of: 28 (n=5), 55 (n=5), 60 (n=5), 70 (n=5) and 114 days of pregnancy (n=5) were obtained. Lectin-histochemistry using fluorescein isothiocyanate-conjugated lectins were employed. Sialic acid residues were detected with low intensity in blood vessels and uterine glands. Variable intensity was found for galactosilated residues, with the lowest intensity for ß-gal (1,3) galNAc residues of PNA binding. However, blood vessels staining was higher than that of glands. The highest staining for glucose and mannose was found in vessels with Con-A lectin, and in glands with VFA. The present results indicate that changes in the glycosilation pattern of glandular and vascular epithelia are related with placental remodeling along gestation. Moreover, the saccharide residues found in vascular and glandular secretions would be part of the nutritional substances provided to the embryos/fetuses during pregnancy.
Fil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Chanique, Analia Maria Luisa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal. Área de Microscopia Electrónica; Argentina
description Since porcine placenta is epitheliochorial and non invasive, maternal and fetal blood flows are separated by six tissue layers. Therefore, the interchange of nutrients between mother and fetuses results indispensable for the success of pregnancy. The glycoconjugates participate in several biological processes, nevertheless their involvement in the materno-fetal interchange that takes place in the placenta is still unclear. The objective was to investigate the glycosilated residues of endometrial glands and blood vessels in placentas of different gestational periods. Crossbred swines from slaughterhouses located in Río Cuarto, Argentina, were used. Samples from placentas of: 28 (n=5), 55 (n=5), 60 (n=5), 70 (n=5) and 114 days of pregnancy (n=5) were obtained. Lectin-histochemistry using fluorescein isothiocyanate-conjugated lectins were employed. Sialic acid residues were detected with low intensity in blood vessels and uterine glands. Variable intensity was found for galactosilated residues, with the lowest intensity for ß-gal (1,3) galNAc residues of PNA binding. However, blood vessels staining was higher than that of glands. The highest staining for glucose and mannose was found in vessels with Con-A lectin, and in glands with VFA. The present results indicate that changes in the glycosilation pattern of glandular and vascular epithelia are related with placental remodeling along gestation. Moreover, the saccharide residues found in vascular and glandular secretions would be part of the nutritional substances provided to the embryos/fetuses during pregnancy.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195839
Sanchis, Eva Gabriela; Cristofolini, Andrea Lorena; Chanique, Analia Maria Luisa; Merkis, Cecilia Inés; Patrón de glicosilación glandular y vascular de la placenta porcina; Veterinaria Organización; Redvet; 13; 3; 3-2012; 1-15
1695-7504
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195839
identifier_str_mv Sanchis, Eva Gabriela; Cristofolini, Andrea Lorena; Chanique, Analia Maria Luisa; Merkis, Cecilia Inés; Patrón de glicosilación glandular y vascular de la placenta porcina; Veterinaria Organización; Redvet; 13; 3; 3-2012; 1-15
1695-7504
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.redalyc.org/articulo.oa?id=63623410007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Veterinaria Organización
publisher.none.fl_str_mv Veterinaria Organización
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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