Intermediate filament protein expression and sugar moieties in normal canine placenta

Autores
Fernández, Patricia Elena; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Gimeno, Eduardo Juan
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.
Facultad de Ciencias Veterinarias
Instituto de Patología "Dr. B. Epstein"
Materia
Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/99697
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/99697
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Intermediate filament protein expression and sugar moieties in normal canine placentaFernández, Patricia ElenaBarbeito, Claudio GustavoPortiansky, Enrique LeoGimeno, Eduardo JuanVeterinariaBitchPlacentaLectinsImmunohistochemistryGlycoconjugatesIn the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.Facultad de Ciencias VeterinariasInstituto de Patología "Dr. B. Epstein"2000-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-6http://sedici.unlp.edu.ar/handle/10915/99697enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/53577info:eu-repo/semantics/altIdentifier/url/http://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_1.htminfo:eu-repo/semantics/altIdentifier/issn/0213-3911info:eu-repo/semantics/altIdentifier/doi/10.14670/HH-15.1info:eu-repo/semantics/altIdentifier/hdl/11336/53577info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:10Zoai:sedici.unlp.edu.ar:10915/99697Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:10.927SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Intermediate filament protein expression and sugar moieties in normal canine placenta
title Intermediate filament protein expression and sugar moieties in normal canine placenta
spellingShingle Intermediate filament protein expression and sugar moieties in normal canine placenta
Fernández, Patricia Elena
Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
title_short Intermediate filament protein expression and sugar moieties in normal canine placenta
title_full Intermediate filament protein expression and sugar moieties in normal canine placenta
title_fullStr Intermediate filament protein expression and sugar moieties in normal canine placenta
title_full_unstemmed Intermediate filament protein expression and sugar moieties in normal canine placenta
title_sort Intermediate filament protein expression and sugar moieties in normal canine placenta
dc.creator.none.fl_str_mv Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author Fernández, Patricia Elena
author_facet Fernández, Patricia Elena
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_role author
author2 Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author2_role author
author
author
dc.subject.none.fl_str_mv Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
topic Veterinaria
Bitch
Placenta
Lectins
Immunohistochemistry
Glycoconjugates
dc.description.none.fl_txt_mv In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.
Facultad de Ciencias Veterinarias
Instituto de Patología "Dr. B. Epstein"
description In the female dog, the placenta is considered zonal, endotheliochorial and labyrinthic. The distribution of the intermediate filaments as well as the surface glycoproteins in the canine placenta are still unknown. The aim of the present study was to provide this information for further understanding of pathological conditions in the bitch. Samples were obtained from normal uterine horns at the end of gestation. Tissues were routinely fixed and stained. Monoclonal antibodies against cytokeratins, vimentin and desmin were used for immunohistochemical staining. UEA-I, PNA, RCA-I, SBA, DBA, WGA and ConA were used for the lectin histochemical staining. A computer morphometrical analysis was made. Statistical analysis was then accomplished. The results showed the maximun immunohistochemical percentage for vimentin in the supra glandular connective tissue, for pancytokeratin in the spongy zone and for desmin in miometrium. SBA showed the highest staining percentage in the gland cells of the spongy zone, while ConA was the highest in the syncytiotrophoblastic cells and gland cells of the deep glandular zone. The results obtained indicate that the lectin binding pattern is partially different from other animal species. On the contrary, the intermediate filament data coincide with similar observations from other mammals.
publishDate 2000
dc.date.none.fl_str_mv 2000-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/99697
url http://sedici.unlp.edu.ar/handle/10915/99697
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/53577
info:eu-repo/semantics/altIdentifier/url/http://www.hh.um.es/Abstracts/Vol_15/15_1/15_1_1.htm
info:eu-repo/semantics/altIdentifier/issn/0213-3911
info:eu-repo/semantics/altIdentifier/doi/10.14670/HH-15.1
info:eu-repo/semantics/altIdentifier/hdl/11336/53577
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1-6
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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