A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus

Autores
Gomez Ribot, Dalmiro Leonardo Antonio; Díaz, Esteban; Fazio, María Victoria; Gresta, Carlos Alberto; Careaga, Valeria; Maier, Marta Silvia; Capobianco, Evangelina Lorena; Jawerbaum, Alicia Sandra
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development, but markers of a pro-inflammatory state when produced in excess. Previous studies have established that gestational diabetes mellitus (GDM) induces a pro-inflammatory intrauterine environment. We hypothesized that a diet enriched in olive oil (OO) regulates MMP-2 and MMP-9 activities in placentas and in maternal and cord blood from women with GDM. Fifty control (C) and GDM women were enrolled after signing an informed consent (protocol approved by the Ethics Committee of Hospital Pirovano, Buenos Aires). All of them followed the WHO diet for pregnancy, and a group of GDM women received a 26 mL-OO addition daily from week 24-28 of gestation until delivery (GDMO group). At delivery, placental villous explants and maternal and cord blood were obtained and stored at -80ºC for zymography analysis. Fatty acids (FAs) profile was evaluated in cord blood by GC-FID. MMP-9 activity was increased in the placentas and in maternal blood of the GDM group (133 and 81 % respectively, p<0.05 vs. C), alterations prevented by the diet enriched in OO (p<0.05 vs. GDM). No changes were found in the MMP-2 activity among the three evaluated groups. Regarding cord blood, MMP-9 and MMP-2 activities were both increased in the GDM group compared to C (90 and 21 % respectively, p<0.05 vs. C), increases prevented by the OO addition (p<0.05 vs. GDM). These changes occurred without major changes in fatty acid composition in the cord blood from the three experimental groups. The diet enriched in OO modulates MMP-9 activity in term placentas and in maternal and cord blood, indicating the capability of this diet to module the pro-inflammatory intrauterine environment induced by GDM. The lack of changes in FFA profile in cord blood suggest that the unsaturated fatty acids provided by the diet exert antiinflammatory effects within the placenta and fetal organs, with possible benefits for the offspring’s later life.
Fil: Gomez Ribot, Dalmiro Leonardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Díaz, Esteban. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Fazio, María Victoria. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Gresta, Carlos Alberto. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Careaga, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Fil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Reunión Anual de las Sociedades de Biociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Protozoología
Materia
PLACENTA
GESTATIONAL DIABETES
DIETARY TREATMENTS
LIPID METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163927
Acceder
id CONICETDig_3c6e582f17085c87115244d5ae9f6d0a
oai_identifier_str oai:ri.conicet.gov.ar:11336/163927
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitusGomez Ribot, Dalmiro Leonardo AntonioDíaz, EstebanFazio, María VictoriaGresta, Carlos AlbertoCareaga, ValeriaMaier, Marta SilviaCapobianco, Evangelina LorenaJawerbaum, Alicia SandraPLACENTAGESTATIONAL DIABETESDIETARY TREATMENTSLIPID METABOLISMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development, but markers of a pro-inflammatory state when produced in excess. Previous studies have established that gestational diabetes mellitus (GDM) induces a pro-inflammatory intrauterine environment. We hypothesized that a diet enriched in olive oil (OO) regulates MMP-2 and MMP-9 activities in placentas and in maternal and cord blood from women with GDM. Fifty control (C) and GDM women were enrolled after signing an informed consent (protocol approved by the Ethics Committee of Hospital Pirovano, Buenos Aires). All of them followed the WHO diet for pregnancy, and a group of GDM women received a 26 mL-OO addition daily from week 24-28 of gestation until delivery (GDMO group). At delivery, placental villous explants and maternal and cord blood were obtained and stored at -80ºC for zymography analysis. Fatty acids (FAs) profile was evaluated in cord blood by GC-FID. MMP-9 activity was increased in the placentas and in maternal blood of the GDM group (133 and 81 % respectively, p<0.05 vs. C), alterations prevented by the diet enriched in OO (p<0.05 vs. GDM). No changes were found in the MMP-2 activity among the three evaluated groups. Regarding cord blood, MMP-9 and MMP-2 activities were both increased in the GDM group compared to C (90 and 21 % respectively, p<0.05 vs. C), increases prevented by the OO addition (p<0.05 vs. GDM). These changes occurred without major changes in fatty acid composition in the cord blood from the three experimental groups. The diet enriched in OO modulates MMP-9 activity in term placentas and in maternal and cord blood, indicating the capability of this diet to module the pro-inflammatory intrauterine environment induced by GDM. The lack of changes in FFA profile in cord blood suggest that the unsaturated fatty acids provided by the diet exert antiinflammatory effects within the placenta and fetal organs, with possible benefits for the offspring’s later life.Fil: Gomez Ribot, Dalmiro Leonardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Díaz, Esteban. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; ArgentinaFil: Fazio, María Victoria. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; ArgentinaFil: Gresta, Carlos Alberto. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; ArgentinaFil: Careaga, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; ArgentinaFil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; ArgentinaFil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaReunión Anual de las Sociedades de BiocienciasMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioSociedad Argentina de ProtozoologíaFundación Revista Medicina2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163927A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus; Reunión Anual de las Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 260-2610025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:31Zoai:ri.conicet.gov.ar:11336/163927instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:31.675CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
title A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
spellingShingle A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
Gomez Ribot, Dalmiro Leonardo Antonio
PLACENTA
GESTATIONAL DIABETES
DIETARY TREATMENTS
LIPID METABOLISM
title_short A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
title_full A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
title_fullStr A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
title_full_unstemmed A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
title_sort A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus
dc.creator.none.fl_str_mv Gomez Ribot, Dalmiro Leonardo Antonio
Díaz, Esteban
Fazio, María Victoria
Gresta, Carlos Alberto
Careaga, Valeria
Maier, Marta Silvia
Capobianco, Evangelina Lorena
Jawerbaum, Alicia Sandra
author Gomez Ribot, Dalmiro Leonardo Antonio
author_facet Gomez Ribot, Dalmiro Leonardo Antonio
Díaz, Esteban
Fazio, María Victoria
Gresta, Carlos Alberto
Careaga, Valeria
Maier, Marta Silvia
Capobianco, Evangelina Lorena
Jawerbaum, Alicia Sandra
author_role author
author2 Díaz, Esteban
Fazio, María Victoria
Gresta, Carlos Alberto
Careaga, Valeria
Maier, Marta Silvia
Capobianco, Evangelina Lorena
Jawerbaum, Alicia Sandra
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PLACENTA
GESTATIONAL DIABETES
DIETARY TREATMENTS
LIPID METABOLISM
topic PLACENTA
GESTATIONAL DIABETES
DIETARY TREATMENTS
LIPID METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development, but markers of a pro-inflammatory state when produced in excess. Previous studies have established that gestational diabetes mellitus (GDM) induces a pro-inflammatory intrauterine environment. We hypothesized that a diet enriched in olive oil (OO) regulates MMP-2 and MMP-9 activities in placentas and in maternal and cord blood from women with GDM. Fifty control (C) and GDM women were enrolled after signing an informed consent (protocol approved by the Ethics Committee of Hospital Pirovano, Buenos Aires). All of them followed the WHO diet for pregnancy, and a group of GDM women received a 26 mL-OO addition daily from week 24-28 of gestation until delivery (GDMO group). At delivery, placental villous explants and maternal and cord blood were obtained and stored at -80ºC for zymography analysis. Fatty acids (FAs) profile was evaluated in cord blood by GC-FID. MMP-9 activity was increased in the placentas and in maternal blood of the GDM group (133 and 81 % respectively, p<0.05 vs. C), alterations prevented by the diet enriched in OO (p<0.05 vs. GDM). No changes were found in the MMP-2 activity among the three evaluated groups. Regarding cord blood, MMP-9 and MMP-2 activities were both increased in the GDM group compared to C (90 and 21 % respectively, p<0.05 vs. C), increases prevented by the OO addition (p<0.05 vs. GDM). These changes occurred without major changes in fatty acid composition in the cord blood from the three experimental groups. The diet enriched in OO modulates MMP-9 activity in term placentas and in maternal and cord blood, indicating the capability of this diet to module the pro-inflammatory intrauterine environment induced by GDM. The lack of changes in FFA profile in cord blood suggest that the unsaturated fatty acids provided by the diet exert antiinflammatory effects within the placenta and fetal organs, with possible benefits for the offspring’s later life.
Fil: Gomez Ribot, Dalmiro Leonardo Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Díaz, Esteban. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Fazio, María Victoria. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Gresta, Carlos Alberto. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Ignacio Pirovano; Argentina
Fil: Careaga, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Fil: Maier, Marta Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Unidad de Microanálisis y Métodos Físicos en Química Orgánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica; Argentina
Fil: Capobianco, Evangelina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Reunión Anual de las Sociedades de Biociencias
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Sociedad Argentina de Protozoología
description Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in placental development, but markers of a pro-inflammatory state when produced in excess. Previous studies have established that gestational diabetes mellitus (GDM) induces a pro-inflammatory intrauterine environment. We hypothesized that a diet enriched in olive oil (OO) regulates MMP-2 and MMP-9 activities in placentas and in maternal and cord blood from women with GDM. Fifty control (C) and GDM women were enrolled after signing an informed consent (protocol approved by the Ethics Committee of Hospital Pirovano, Buenos Aires). All of them followed the WHO diet for pregnancy, and a group of GDM women received a 26 mL-OO addition daily from week 24-28 of gestation until delivery (GDMO group). At delivery, placental villous explants and maternal and cord blood were obtained and stored at -80ºC for zymography analysis. Fatty acids (FAs) profile was evaluated in cord blood by GC-FID. MMP-9 activity was increased in the placentas and in maternal blood of the GDM group (133 and 81 % respectively, p<0.05 vs. C), alterations prevented by the diet enriched in OO (p<0.05 vs. GDM). No changes were found in the MMP-2 activity among the three evaluated groups. Regarding cord blood, MMP-9 and MMP-2 activities were both increased in the GDM group compared to C (90 and 21 % respectively, p<0.05 vs. C), increases prevented by the OO addition (p<0.05 vs. GDM). These changes occurred without major changes in fatty acid composition in the cord blood from the three experimental groups. The diet enriched in OO modulates MMP-9 activity in term placentas and in maternal and cord blood, indicating the capability of this diet to module the pro-inflammatory intrauterine environment induced by GDM. The lack of changes in FFA profile in cord blood suggest that the unsaturated fatty acids provided by the diet exert antiinflammatory effects within the placenta and fetal organs, with possible benefits for the offspring’s later life.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163927
A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus; Reunión Anual de las Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 260-261
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163927
identifier_str_mv A maternal diet enriched in olive oil regulates matrix metalloproteinases activity in maternal and cord blood and in term placentas from women with gestational diabetes mellitus; Reunión Anual de las Sociedades de Biociencias; Mar del Plata; Argentina; 2019; 260-261
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/indices-de-2010-a-2019/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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