Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion

Autores
Funes, Abi Karenina; Colombo, Regina Lucía; Boarelli, Paola; Monclus, Maria de Los Angeles; Conte, María Inés; Cresciteli, Julieta; Cortese, Leandro; Saez Lancellotti, Tania Emilce Estefania; Fornes, Miguel Walter
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fat diet promotes hypercholesterolemia and infertility in adult male rabbits without obesity. This metabolic-reproductive disorder is corrected with a decrease in fat diet accompanied with the intake of extra virgin olive oil (AOVE). The underlying cellular and molecular mechanisms are still under research. The general regulation of cholesterol also relies on intracellular regulation. The cellular / molecular balance of cholesterol levels depends on the SREBP 2 pathway and its dependent molecules, such as LDLr or Methyl-Coenzyme A, which are involved in de novo synthesis of this lipid. The high fat diet promoted a decrease in the expression of SREBP 2 (mRNA and protein), in rabbits after 3 months compared to control animals that consumed balanced diet for rabbits (GEPSA ®), in both liver and testicle. This drop in expression is more pronounced at 6 months of diet. Interestingly, the addition of AOVE to the diet promoted an increase in the expression of SREBP 2 (both mRNA and protein) in both tissues, hepatic and seminiferous tubule. In addition, it was possible to observe the subcellular localization of the SREBP-2 protein in testes and hepatocytes by immunofluorescence (IFI). In the testicle, the SREBP 2 protein was located in interstitial cells and, depending on the stage of the seminiferous epithelial cycle, Sertoli and/or germline cells. We can infer that dietary fat / circulating cholesterol affects the hepatic and testicular levels, generating alterations such as fatty liver and germinal disorders with abnormal sperm cells observed in previous studies. These changes are due to an imbalance of the intracellular cholesterol regulatory pathway. The AOVE contributes in the correction of the hepatic and testicular histo-physiology.
Fil: Funes, Abi Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Colombo, Regina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Boarelli, Paola. Universidad "Juan Agustín Maza"; Argentina
Fil: Monclus, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; Argentina
Fil: Conte, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cresciteli, Julieta. Universidad del Aconcagua. Facultad de Cs.Medicas; Argentina
Fil: Cortese, Leandro. Universidad del Aconcagua. Facultad de Cs.Medicas; Argentina
Fil: Saez Lancellotti, Tania Emilce Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; Argentina
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
HIPERCOLESTEROLEMIA
ACEITE DE OLIVA
SREBP 2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/236964
Acceder
id CONICETDig_fbfdf64c420bd9307bde3453bfcd7202
oai_identifier_str oai:ri.conicet.gov.ar:11336/236964
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittionFunes, Abi KareninaColombo, Regina LucíaBoarelli, PaolaMonclus, Maria de Los AngelesConte, María InésCresciteli, JulietaCortese, LeandroSaez Lancellotti, Tania Emilce EstefaniaFornes, Miguel WalterHIPERCOLESTEROLEMIAACEITE DE OLIVASREBP 2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fat diet promotes hypercholesterolemia and infertility in adult male rabbits without obesity. This metabolic-reproductive disorder is corrected with a decrease in fat diet accompanied with the intake of extra virgin olive oil (AOVE). The underlying cellular and molecular mechanisms are still under research. The general regulation of cholesterol also relies on intracellular regulation. The cellular / molecular balance of cholesterol levels depends on the SREBP 2 pathway and its dependent molecules, such as LDLr or Methyl-Coenzyme A, which are involved in de novo synthesis of this lipid. The high fat diet promoted a decrease in the expression of SREBP 2 (mRNA and protein), in rabbits after 3 months compared to control animals that consumed balanced diet for rabbits (GEPSA ®), in both liver and testicle. This drop in expression is more pronounced at 6 months of diet. Interestingly, the addition of AOVE to the diet promoted an increase in the expression of SREBP 2 (both mRNA and protein) in both tissues, hepatic and seminiferous tubule. In addition, it was possible to observe the subcellular localization of the SREBP-2 protein in testes and hepatocytes by immunofluorescence (IFI). In the testicle, the SREBP 2 protein was located in interstitial cells and, depending on the stage of the seminiferous epithelial cycle, Sertoli and/or germline cells. We can infer that dietary fat / circulating cholesterol affects the hepatic and testicular levels, generating alterations such as fatty liver and germinal disorders with abnormal sperm cells observed in previous studies. These changes are due to an imbalance of the intracellular cholesterol regulatory pathway. The AOVE contributes in the correction of the hepatic and testicular histo-physiology.Fil: Funes, Abi Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Colombo, Regina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Boarelli, Paola. Universidad "Juan Agustín Maza"; ArgentinaFil: Monclus, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; ArgentinaFil: Conte, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Cresciteli, Julieta. Universidad del Aconcagua. Facultad de Cs.Medicas; ArgentinaFil: Cortese, Leandro. Universidad del Aconcagua. Facultad de Cs.Medicas; ArgentinaFil: Saez Lancellotti, Tania Emilce Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; ArgentinaFil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaXXXVI Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/236964Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-450327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/08/Biocell-2018.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:24:10Zoai:ri.conicet.gov.ar:11336/236964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:24:11.159CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
title Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
spellingShingle Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
Funes, Abi Karenina
HIPERCOLESTEROLEMIA
ACEITE DE OLIVA
SREBP 2
title_short Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
title_full Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
title_fullStr Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
title_full_unstemmed Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
title_sort Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion
dc.creator.none.fl_str_mv Funes, Abi Karenina
Colombo, Regina Lucía
Boarelli, Paola
Monclus, Maria de Los Angeles
Conte, María Inés
Cresciteli, Julieta
Cortese, Leandro
Saez Lancellotti, Tania Emilce Estefania
Fornes, Miguel Walter
author Funes, Abi Karenina
author_facet Funes, Abi Karenina
Colombo, Regina Lucía
Boarelli, Paola
Monclus, Maria de Los Angeles
Conte, María Inés
Cresciteli, Julieta
Cortese, Leandro
Saez Lancellotti, Tania Emilce Estefania
Fornes, Miguel Walter
author_role author
author2 Colombo, Regina Lucía
Boarelli, Paola
Monclus, Maria de Los Angeles
Conte, María Inés
Cresciteli, Julieta
Cortese, Leandro
Saez Lancellotti, Tania Emilce Estefania
Fornes, Miguel Walter
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HIPERCOLESTEROLEMIA
ACEITE DE OLIVA
SREBP 2
topic HIPERCOLESTEROLEMIA
ACEITE DE OLIVA
SREBP 2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fat diet promotes hypercholesterolemia and infertility in adult male rabbits without obesity. This metabolic-reproductive disorder is corrected with a decrease in fat diet accompanied with the intake of extra virgin olive oil (AOVE). The underlying cellular and molecular mechanisms are still under research. The general regulation of cholesterol also relies on intracellular regulation. The cellular / molecular balance of cholesterol levels depends on the SREBP 2 pathway and its dependent molecules, such as LDLr or Methyl-Coenzyme A, which are involved in de novo synthesis of this lipid. The high fat diet promoted a decrease in the expression of SREBP 2 (mRNA and protein), in rabbits after 3 months compared to control animals that consumed balanced diet for rabbits (GEPSA ®), in both liver and testicle. This drop in expression is more pronounced at 6 months of diet. Interestingly, the addition of AOVE to the diet promoted an increase in the expression of SREBP 2 (both mRNA and protein) in both tissues, hepatic and seminiferous tubule. In addition, it was possible to observe the subcellular localization of the SREBP-2 protein in testes and hepatocytes by immunofluorescence (IFI). In the testicle, the SREBP 2 protein was located in interstitial cells and, depending on the stage of the seminiferous epithelial cycle, Sertoli and/or germline cells. We can infer that dietary fat / circulating cholesterol affects the hepatic and testicular levels, generating alterations such as fatty liver and germinal disorders with abnormal sperm cells observed in previous studies. These changes are due to an imbalance of the intracellular cholesterol regulatory pathway. The AOVE contributes in the correction of the hepatic and testicular histo-physiology.
Fil: Funes, Abi Karenina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Colombo, Regina Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Boarelli, Paola. Universidad "Juan Agustín Maza"; Argentina
Fil: Monclus, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; Argentina
Fil: Conte, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Cresciteli, Julieta. Universidad del Aconcagua. Facultad de Cs.Medicas; Argentina
Fil: Cortese, Leandro. Universidad del Aconcagua. Facultad de Cs.Medicas; Argentina
Fil: Saez Lancellotti, Tania Emilce Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad del Aconcagua; Argentina
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Fat diet promotes hypercholesterolemia and infertility in adult male rabbits without obesity. This metabolic-reproductive disorder is corrected with a decrease in fat diet accompanied with the intake of extra virgin olive oil (AOVE). The underlying cellular and molecular mechanisms are still under research. The general regulation of cholesterol also relies on intracellular regulation. The cellular / molecular balance of cholesterol levels depends on the SREBP 2 pathway and its dependent molecules, such as LDLr or Methyl-Coenzyme A, which are involved in de novo synthesis of this lipid. The high fat diet promoted a decrease in the expression of SREBP 2 (mRNA and protein), in rabbits after 3 months compared to control animals that consumed balanced diet for rabbits (GEPSA ®), in both liver and testicle. This drop in expression is more pronounced at 6 months of diet. Interestingly, the addition of AOVE to the diet promoted an increase in the expression of SREBP 2 (both mRNA and protein) in both tissues, hepatic and seminiferous tubule. In addition, it was possible to observe the subcellular localization of the SREBP-2 protein in testes and hepatocytes by immunofluorescence (IFI). In the testicle, the SREBP 2 protein was located in interstitial cells and, depending on the stage of the seminiferous epithelial cycle, Sertoli and/or germline cells. We can infer that dietary fat / circulating cholesterol affects the hepatic and testicular levels, generating alterations such as fatty liver and germinal disorders with abnormal sperm cells observed in previous studies. These changes are due to an imbalance of the intracellular cholesterol regulatory pathway. The AOVE contributes in the correction of the hepatic and testicular histo-physiology.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/236964
Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-45
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/236964
identifier_str_mv Srebp 2 (sterol - regulatory - binding - protein 2) pathway in testis and liver of hypercholesterolemic rabbits promoted by fat diet protection by olibe oil adittion; XXXVI Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019; 45-45
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
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