Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
- Autores
- Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.
Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; España
Fil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; España
Fil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; España - Materia
-
Srebp
Adipocyte - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/11035
Ver los metadatos del registro completo
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Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescenceAlvarez, María SoledadFernández Alvarez, Ana JuliaCucarella, CarmeCasado Pinna, MartaSrebpAdipocytehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; EspañaFil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; EspañaFil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; EspañaElsevier2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11035Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-560006-291Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X14005567info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2014.03.104info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:34Zoai:ri.conicet.gov.ar:11336/11035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:34.429CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
title |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
spellingShingle |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence Alvarez, María Soledad Srebp Adipocyte |
title_short |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
title_full |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
title_fullStr |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
title_full_unstemmed |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
title_sort |
Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence |
dc.creator.none.fl_str_mv |
Alvarez, María Soledad Fernández Alvarez, Ana Julia Cucarella, Carme Casado Pinna, Marta |
author |
Alvarez, María Soledad |
author_facet |
Alvarez, María Soledad Fernández Alvarez, Ana Julia Cucarella, Carme Casado Pinna, Marta |
author_role |
author |
author2 |
Fernández Alvarez, Ana Julia Cucarella, Carme Casado Pinna, Marta |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Srebp Adipocyte |
topic |
Srebp Adipocyte |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation. Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; España Fil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina Fil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; España Fil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; España |
description |
Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/11035 Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-56 0006-291X |
url |
http://hdl.handle.net/11336/11035 |
identifier_str_mv |
Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-56 0006-291X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X14005567 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2014.03.104 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1846082582389719040 |
score |
13.22299 |