Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence

Autores
Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.
Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; España
Fil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; España
Fil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; España
Materia
Srebp
Adipocyte
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11035
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/11035
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescenceAlvarez, María SoledadFernández Alvarez, Ana JuliaCucarella, CarmeCasado Pinna, MartaSrebpAdipocytehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; EspañaFil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; EspañaFil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; EspañaElsevier2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11035Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-560006-291Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X14005567info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2014.03.104info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:34Zoai:ri.conicet.gov.ar:11336/11035instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:34.429CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
title Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
spellingShingle Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
Alvarez, María Soledad
Srebp
Adipocyte
title_short Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
title_full Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
title_fullStr Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
title_full_unstemmed Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
title_sort Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence
dc.creator.none.fl_str_mv Alvarez, María Soledad
Fernández Alvarez, Ana Julia
Cucarella, Carme
Casado Pinna, Marta
author Alvarez, María Soledad
author_facet Alvarez, María Soledad
Fernández Alvarez, Ana Julia
Cucarella, Carme
Casado Pinna, Marta
author_role author
author2 Fernández Alvarez, Ana Julia
Cucarella, Carme
Casado Pinna, Marta
author2_role author
author
author
dc.subject.none.fl_str_mv Srebp
Adipocyte
topic Srebp
Adipocyte
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.
Fil: Alvarez, María Soledad. Consejo Superior de Investigaciones Cientificas; España
Fil: Fernández Alvarez, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Cucarella, Carme. Consejo Superior de Investigaciones Cientificas; España
Fil: Casado Pinna, Marta. Consejo Superior de Investigaciones Cientificas; España
description Sterol regulatory element binding proteins (SREBP), encoded by the Srebf1 and Srebf2 genes, are important regulators of genes involved in cholesterol and fatty acid metabolism. Whereas SREBP-2 controls the cholesterol synthesis, SREBP-1 proteins (-1a and -1c) function as the central hubs in lipid metabolism. Despite the key function of these transcription factors to promote adipocyte differentiation, the roles of SREBP-1 proteins during the preadipocyte state remain unknown. Here, we evaluate the role of SREBP-1 in preadipocyte proliferation using RNA interference technology. Knockdown of the SREBP-1a gene decreased the proliferation rate in human SGBS preadipocyte cell strain without inducing senescence. Furthermore, our data identified retinoblastoma binding protein 8 and cyclin-dependent kinase inhibitor 3 genes as new potential SREBP-1 targets, in addition to cyclin-dependent kinase inhibitor 1A which had already been described as a gene regulated by SREBP-1a. These data suggested a new role of SREBP-1 in adipogenesis via regulation of preadipocyte proliferation.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11035
Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-56
0006-291X
url http://hdl.handle.net/11336/11035
identifier_str_mv Alvarez, María Soledad; Fernández Alvarez, Ana Julia; Cucarella, Carme; Casado Pinna, Marta; Stable SREBP-1a knockdown decreases the cell proliferation rate in human preadipocyte cells without inducing senescence; Elsevier; Biochemical And Biophysical Research Communications; 447; 1; 4-2014; 51-56
0006-291X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X14005567
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbrc.2014.03.104
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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