Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke

Autores
Santarossa, Debora Gimena; Talio, María Carolina; Fernandez, Liliana Patricia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work has been to evaluate the aluminium Al(III) traces contents in 24-hours urine samples from subjects with different tobacco smoke exposition using a new methodology using 1,4-dihydroxy-9,10-anthraquinone (Quinizarine, QZ) as a fluorosphore. Biological samples were tested using commercial reagent strips and clinical parameters. Al(III) was determined complexing with QZ followed by a solid phase extraction step using Nylon membranes as solid support. The analyte was subsequently quantified by solid surface fluorescence (SSF, λem= 573, λexc= 490) with a detection limit of 0.88 µg L-1 and quantification limit of 2.69 µg L-1. The calibration curve was linear from 2.69 to 499.13 µg L-1 Al(III) (R2 =0.9973). Urine samples were successfully analysed with an average recovery close to 100%. Solid phase extraction step showed efficacy to eliminate foreign ions and the highly fluorescent matrix own of urine. Results were validated by electrothermal atomic absorption spectrometry (ETAAS) with an adequate concordance. The new methodology has low operation cost with simple instrumentation and without organic solvent.
Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
Materia
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87355
Acceder
id CONICETDig_f9b1173fdc2a6ddafab5d8c36c4bc035
oai_identifier_str oai:ri.conicet.gov.ar:11336/87355
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco SmokeSantarossa, Debora GimenaTalio, María CarolinaFernandez, Liliana PatriciaTOBACCOALUMINIUM TRACESQUINIZARINESOLID PHASE EXTRACTIONSOLID SURFACE FLUORESCENCEURINE SAMPLESTOBACCOALUMINIUM TRACESQUINIZARINESOLID PHASE EXTRACTIONSOLID SURFACE FLUORESCENCEURINE SAMPLEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of this work has been to evaluate the aluminium Al(III) traces contents in 24-hours urine samples from subjects with different tobacco smoke exposition using a new methodology using 1,4-dihydroxy-9,10-anthraquinone (Quinizarine, QZ) as a fluorosphore. Biological samples were tested using commercial reagent strips and clinical parameters. Al(III) was determined complexing with QZ followed by a solid phase extraction step using Nylon membranes as solid support. The analyte was subsequently quantified by solid surface fluorescence (SSF, λem= 573, λexc= 490) with a detection limit of 0.88 µg L-1 and quantification limit of 2.69 µg L-1. The calibration curve was linear from 2.69 to 499.13 µg L-1 Al(III) (R2 =0.9973). Urine samples were successfully analysed with an average recovery close to 100%. Solid phase extraction step showed efficacy to eliminate foreign ions and the highly fluorescent matrix own of urine. Results were validated by electrothermal atomic absorption spectrometry (ETAAS) with an adequate concordance. The new methodology has low operation cost with simple instrumentation and without organic solvent.Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; ArgentinaScientific Research2018-10-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87355Santarossa, Debora Gimena; Talio, María Carolina; Fernandez, Liliana Patricia; Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke; Scientific Research; American Journal Analitycal Chemistry; 09; 10; 25-10-2018; 514-5282156-82512156-8278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajac.2018.910038info:eu-repo/semantics/altIdentifier/doi/10.4236/ajac.2018.910038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:20Zoai:ri.conicet.gov.ar:11336/87355instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:20.685CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
title Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
spellingShingle Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
Santarossa, Debora Gimena
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
title_short Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
title_full Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
title_fullStr Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
title_full_unstemmed Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
title_sort Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke
dc.creator.none.fl_str_mv Santarossa, Debora Gimena
Talio, María Carolina
Fernandez, Liliana Patricia
author Santarossa, Debora Gimena
author_facet Santarossa, Debora Gimena
Talio, María Carolina
Fernandez, Liliana Patricia
author_role author
author2 Talio, María Carolina
Fernandez, Liliana Patricia
author2_role author
author
dc.subject.none.fl_str_mv TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
topic TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
TOBACCO
ALUMINIUM TRACES
QUINIZARINE
SOLID PHASE EXTRACTION
SOLID SURFACE FLUORESCENCE
URINE SAMPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work has been to evaluate the aluminium Al(III) traces contents in 24-hours urine samples from subjects with different tobacco smoke exposition using a new methodology using 1,4-dihydroxy-9,10-anthraquinone (Quinizarine, QZ) as a fluorosphore. Biological samples were tested using commercial reagent strips and clinical parameters. Al(III) was determined complexing with QZ followed by a solid phase extraction step using Nylon membranes as solid support. The analyte was subsequently quantified by solid surface fluorescence (SSF, λem= 573, λexc= 490) with a detection limit of 0.88 µg L-1 and quantification limit of 2.69 µg L-1. The calibration curve was linear from 2.69 to 499.13 µg L-1 Al(III) (R2 =0.9973). Urine samples were successfully analysed with an average recovery close to 100%. Solid phase extraction step showed efficacy to eliminate foreign ions and the highly fluorescent matrix own of urine. Results were validated by electrothermal atomic absorption spectrometry (ETAAS) with an adequate concordance. The new methodology has low operation cost with simple instrumentation and without organic solvent.
Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Talio, María Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Química Analítica; Argentina
description The aim of this work has been to evaluate the aluminium Al(III) traces contents in 24-hours urine samples from subjects with different tobacco smoke exposition using a new methodology using 1,4-dihydroxy-9,10-anthraquinone (Quinizarine, QZ) as a fluorosphore. Biological samples were tested using commercial reagent strips and clinical parameters. Al(III) was determined complexing with QZ followed by a solid phase extraction step using Nylon membranes as solid support. The analyte was subsequently quantified by solid surface fluorescence (SSF, λem= 573, λexc= 490) with a detection limit of 0.88 µg L-1 and quantification limit of 2.69 µg L-1. The calibration curve was linear from 2.69 to 499.13 µg L-1 Al(III) (R2 =0.9973). Urine samples were successfully analysed with an average recovery close to 100%. Solid phase extraction step showed efficacy to eliminate foreign ions and the highly fluorescent matrix own of urine. Results were validated by electrothermal atomic absorption spectrometry (ETAAS) with an adequate concordance. The new methodology has low operation cost with simple instrumentation and without organic solvent.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87355
Santarossa, Debora Gimena; Talio, María Carolina; Fernandez, Liliana Patricia; Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke; Scientific Research; American Journal Analitycal Chemistry; 09; 10; 25-10-2018; 514-528
2156-8251
2156-8278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87355
identifier_str_mv Santarossa, Debora Gimena; Talio, María Carolina; Fernandez, Liliana Patricia; Green Photoluminescent Methodology for Aluminium Traces Quantification in 24-Hour Urine of Subjects with Different Exposition to Tobacco Smoke; Scientific Research; American Journal Analitycal Chemistry; 09; 10; 25-10-2018; 514-528
2156-8251
2156-8278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajac.2018.910038
info:eu-repo/semantics/altIdentifier/doi/10.4236/ajac.2018.910038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782149919768576
score 12.982451

Publicaciones similares