Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples

Autores
Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Materia
AMILORIDE
FLUORESCENCE
FUROSEMIDE
NYLON MEMBRANE
SOLID PHASE EXTRACTION
URINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92845
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92845
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samplesPeralta, Cecilia MarianaFernandez, Liliana PatriciaMasi, Adriana NoemiAMILORIDEFLUORESCENCEFUROSEMIDENYLON MEMBRANESOLID PHASE EXTRACTIONURINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaFil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaElsevier Science2011-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92845Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-430026-265X0026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2010.10.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X10002158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:28Zoai:ri.conicet.gov.ar:11336/92845instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:29.056CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
title Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
spellingShingle Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
Peralta, Cecilia Mariana
AMILORIDE
FLUORESCENCE
FUROSEMIDE
NYLON MEMBRANE
SOLID PHASE EXTRACTION
URINE
title_short Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
title_full Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
title_fullStr Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
title_full_unstemmed Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
title_sort Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
dc.creator.none.fl_str_mv Peralta, Cecilia Mariana
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author Peralta, Cecilia Mariana
author_facet Peralta, Cecilia Mariana
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author_role author
author2 Fernandez, Liliana Patricia
Masi, Adriana Noemi
author2_role author
author
dc.subject.none.fl_str_mv AMILORIDE
FLUORESCENCE
FUROSEMIDE
NYLON MEMBRANE
SOLID PHASE EXTRACTION
URINE
topic AMILORIDE
FLUORESCENCE
FUROSEMIDE
NYLON MEMBRANE
SOLID PHASE EXTRACTION
URINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
description A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.
publishDate 2011
dc.date.none.fl_str_mv 2011-05-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92845
Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-43
0026-265X
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92845
identifier_str_mv Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-43
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2010.10.009
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X10002158
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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