Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples
- Autores
- Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina
Fil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina - Materia
-
AMILORIDE
FLUORESCENCE
FUROSEMIDE
NYLON MEMBRANE
SOLID PHASE EXTRACTION
URINE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/92845
Ver los metadatos del registro completo
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Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samplesPeralta, Cecilia MarianaFernandez, Liliana PatriciaMasi, Adriana NoemiAMILORIDEFLUORESCENCEFUROSEMIDENYLON MEMBRANESOLID PHASE EXTRACTIONURINEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaFil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; ArgentinaElsevier Science2011-05-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92845Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-430026-265X0026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2010.10.009info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X10002158info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:22Zoai:ri.conicet.gov.ar:11336/92845instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:23.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
title |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
spellingShingle |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples Peralta, Cecilia Mariana AMILORIDE FLUORESCENCE FUROSEMIDE NYLON MEMBRANE SOLID PHASE EXTRACTION URINE |
title_short |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
title_full |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
title_fullStr |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
title_full_unstemmed |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
title_sort |
Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples |
dc.creator.none.fl_str_mv |
Peralta, Cecilia Mariana Fernandez, Liliana Patricia Masi, Adriana Noemi |
author |
Peralta, Cecilia Mariana |
author_facet |
Peralta, Cecilia Mariana Fernandez, Liliana Patricia Masi, Adriana Noemi |
author_role |
author |
author2 |
Fernandez, Liliana Patricia Masi, Adriana Noemi |
author2_role |
author author |
dc.subject.none.fl_str_mv |
AMILORIDE FLUORESCENCE FUROSEMIDE NYLON MEMBRANE SOLID PHASE EXTRACTION URINE |
topic |
AMILORIDE FLUORESCENCE FUROSEMIDE NYLON MEMBRANE SOLID PHASE EXTRACTION URINE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory. Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina Fil: Masi, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química de San Luis; Argentina |
description |
A diuretic is a substance widely prohibited in competition sports, by the World Anti-Doping Agency (WADA). In this paper, a sensitive, rapid and convenient analytical solid phase extraction-fluorimetric method for the determination of furosemide (FUR) and amiloride hydrochloride (AMI) diuretics in human urine was developed. FUR and AMI exhibit overlapped fluorescent spectra and urine produces background fluorescence that precludes the direct determination of these diuretics by conventional fluorimetry. AMI was adsorbed using nylon membranes whereas; FUR was detected in aqueous solutions. The optimum analytical conditions for AMI and FUR assay were established. Excitation wavelengths of 365nm and 237nm and emission wavelengths of 406 and 415nm were used for AMI and FUR, respectively. The calibration graphs are linear in the range of 3.7×10-4 to 0.8 μgmL-1 and 1.2×10-3 to 4.0 μgmL-1, for AMI and FUR, respectively, with a detection limit of 1.1×10-4 and 3.5×10-4μgmL-1 (S/N=3). These concentrations correspond well with the range of the drugs in urine. AMI and FUR were accurately quantified in doped urine samples and urine of subjects under medical treatment with diuretic. The results were validated by recovery test, being highly satisfactory. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-05-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/92845 Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-43 0026-265X 0026-265X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/92845 |
identifier_str_mv |
Peralta, Cecilia Mariana; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Solid phase extraction using nylon membranes with fluorescence detection as a fast and sensitive method for Amiloride and Furosemide determination in urine samples; Elsevier Science; Microchemical Journal; 98; 1; 6-5-2011; 39-43 0026-265X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2010.10.009 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0026265X10002158 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269221671665664 |
score |
13.13397 |