Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

• Autores: Alfarhani, Bassam; Al Tameemi, Maha; Schenone, Agustina Violeta; Goicoechea, Hector Casimiro; Barbosa Jr., Fernando; Campiglia, Andres D.
• Año de publicación: 2016
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Benzo[A]Pyrene; Benzo[A]Pyrene Metabolites; Solid-Phase Extraction; Room-Temperature Fluorescence; Urine Analysis; Asymmetric Least Squares
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Benzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[ a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.
Fil: Alfarhani, Bassam. University Of Central Florida; Estados Unidos
Fil: Al Tameemi, Maha. University Of Central Florida; Estados Unidos
Fil: Schenone, Agustina Violeta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Química. Cátedra de Química Analítica; Argentina
Fil: Barbosa Jr., Fernando. Universidade de Sao Paulo; Brasil
Fil: Campiglia, Andres D.. University Of Central Florida; Estados Unidos