Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring

Autores
Prado Spalm, Facundo Heber; Cuervo Sanchez, Marie Lucia; Furland, Natalia Edith; Valles, Ana Sofia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Since fructose (F) consumption has been linked to the aetiology of metabolic syndrome (MetS), we studied the implications of long- term effects of F consumption by dams on rat offspring neurode- velopment and neurobehavior. Two-month-old Wistar rats were fed standard chow and drank water (control, C) or 20% F (ad libitum) for 10 weeks before mating and throughout pregnancy and lactation. In dams, macronutrient and caloric intake were calculated and blood samples were collected for biochemical analysis. At postnatal day 1 (PN1) oxidative and inflammatory brain status were evaluated in male (♂) and female (♀) pups. The remaining litters (reduced to 8 pups with equal sex ratios) were reassigned to a nursing dam from the C group (NC) or from the F group (NF). Offspring were evaluat- ed for sensorimotor reflexes, reciprocal social interaction, memory retention, novel object recognition and nociception. A p value <0.05 was regarded as statistically significant. F consumption in dams in- duced MetS, increased caloric and carbohydrate intake and reduced protein and fat consumption. Weight, waist circumference and fat % were also higher in F dams. At PN1, ♀ from the F group showed enhanced lipid peroxidation (TBARS) levels, elevated catalase ac- tivity and increased superoxide dismutase protein expression in the whole brain. Neuroinflammation was corroborated by increased pro- tein expression of cyclooxygenase 2. The acquisition of developing milestones was delayed in ♀ from F-NC and F-NF groups. In com- parison with C-NC litter, aggressive social behavior increased while memory retention and nociception were reduced in offspring from F-NC. The ability of recognizing a previously presented stimulus was affected only in ♂ from the F-NC group. These findings suggest that F-induced maternal MetS results in offspring neurodevelopmen- tal delay and neurobehavioral changes probably associated with early brain oxidative stress and inflammation. Interestingly, offspring gender differences were noted
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
dieta alta en fructosa
síndrome metabólico materno
neurodesarrollo
comportamiento
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227135
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspringPrado Spalm, Facundo HeberCuervo Sanchez, Marie LuciaFurland, Natalia EdithValles, Ana Sofiadieta alta en fructosasíndrome metabólico maternoneurodesarrollocomportamientohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Since fructose (F) consumption has been linked to the aetiology of metabolic syndrome (MetS), we studied the implications of long- term effects of F consumption by dams on rat offspring neurode- velopment and neurobehavior. Two-month-old Wistar rats were fed standard chow and drank water (control, C) or 20% F (ad libitum) for 10 weeks before mating and throughout pregnancy and lactation. In dams, macronutrient and caloric intake were calculated and blood samples were collected for biochemical analysis. At postnatal day 1 (PN1) oxidative and inflammatory brain status were evaluated in male (♂) and female (♀) pups. The remaining litters (reduced to 8 pups with equal sex ratios) were reassigned to a nursing dam from the C group (NC) or from the F group (NF). Offspring were evaluat- ed for sensorimotor reflexes, reciprocal social interaction, memory retention, novel object recognition and nociception. A p value <0.05 was regarded as statistically significant. F consumption in dams in- duced MetS, increased caloric and carbohydrate intake and reduced protein and fat consumption. Weight, waist circumference and fat % were also higher in F dams. At PN1, ♀ from the F group showed enhanced lipid peroxidation (TBARS) levels, elevated catalase ac- tivity and increased superoxide dismutase protein expression in the whole brain. Neuroinflammation was corroborated by increased pro- tein expression of cyclooxygenase 2. The acquisition of developing milestones was delayed in ♀ from F-NC and F-NF groups. In com- parison with C-NC litter, aggressive social behavior increased while memory retention and nociception were reduced in offspring from F-NC. The ability of recognizing a previously presented stimulus was affected only in ♂ from the F-NC group. These findings suggest that F-induced maternal MetS results in offspring neurodevelopmen- tal delay and neurobehavioral changes probably associated with early brain oxidative stress and inflammation. Interestingly, offspring gender differences were notedFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista MedicinaAlonso, Daniel FernandoMalchiodi, Emilio LuisVila Petroff, Martin GerardeLamb, Caroline Ana2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227135Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring; Reunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 185-1850025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:28Zoai:ri.conicet.gov.ar:11336/227135instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:28.309CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
title Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
spellingShingle Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
Prado Spalm, Facundo Heber
dieta alta en fructosa
síndrome metabólico materno
neurodesarrollo
comportamiento
title_short Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
title_full Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
title_fullStr Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
title_full_unstemmed Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
title_sort Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring
dc.creator.none.fl_str_mv Prado Spalm, Facundo Heber
Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author Prado Spalm, Facundo Heber
author_facet Prado Spalm, Facundo Heber
Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author_role author
author2 Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author2_role author
author
author
dc.contributor.none.fl_str_mv Alonso, Daniel Fernando
Malchiodi, Emilio Luis
Vila Petroff, Martin Gerarde
Lamb, Caroline Ana
dc.subject.none.fl_str_mv dieta alta en fructosa
síndrome metabólico materno
neurodesarrollo
comportamiento
topic dieta alta en fructosa
síndrome metabólico materno
neurodesarrollo
comportamiento
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Since fructose (F) consumption has been linked to the aetiology of metabolic syndrome (MetS), we studied the implications of long- term effects of F consumption by dams on rat offspring neurode- velopment and neurobehavior. Two-month-old Wistar rats were fed standard chow and drank water (control, C) or 20% F (ad libitum) for 10 weeks before mating and throughout pregnancy and lactation. In dams, macronutrient and caloric intake were calculated and blood samples were collected for biochemical analysis. At postnatal day 1 (PN1) oxidative and inflammatory brain status were evaluated in male (♂) and female (♀) pups. The remaining litters (reduced to 8 pups with equal sex ratios) were reassigned to a nursing dam from the C group (NC) or from the F group (NF). Offspring were evaluat- ed for sensorimotor reflexes, reciprocal social interaction, memory retention, novel object recognition and nociception. A p value <0.05 was regarded as statistically significant. F consumption in dams in- duced MetS, increased caloric and carbohydrate intake and reduced protein and fat consumption. Weight, waist circumference and fat % were also higher in F dams. At PN1, ♀ from the F group showed enhanced lipid peroxidation (TBARS) levels, elevated catalase ac- tivity and increased superoxide dismutase protein expression in the whole brain. Neuroinflammation was corroborated by increased pro- tein expression of cyclooxygenase 2. The acquisition of developing milestones was delayed in ♀ from F-NC and F-NF groups. In com- parison with C-NC litter, aggressive social behavior increased while memory retention and nociception were reduced in offspring from F-NC. The ability of recognizing a previously presented stimulus was affected only in ♂ from the F-NC group. These findings suggest that F-induced maternal MetS results in offspring neurodevelopmen- tal delay and neurobehavioral changes probably associated with early brain oxidative stress and inflammation. Interestingly, offspring gender differences were noted
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Since fructose (F) consumption has been linked to the aetiology of metabolic syndrome (MetS), we studied the implications of long- term effects of F consumption by dams on rat offspring neurode- velopment and neurobehavior. Two-month-old Wistar rats were fed standard chow and drank water (control, C) or 20% F (ad libitum) for 10 weeks before mating and throughout pregnancy and lactation. In dams, macronutrient and caloric intake were calculated and blood samples were collected for biochemical analysis. At postnatal day 1 (PN1) oxidative and inflammatory brain status were evaluated in male (♂) and female (♀) pups. The remaining litters (reduced to 8 pups with equal sex ratios) were reassigned to a nursing dam from the C group (NC) or from the F group (NF). Offspring were evaluat- ed for sensorimotor reflexes, reciprocal social interaction, memory retention, novel object recognition and nociception. A p value <0.05 was regarded as statistically significant. F consumption in dams in- duced MetS, increased caloric and carbohydrate intake and reduced protein and fat consumption. Weight, waist circumference and fat % were also higher in F dams. At PN1, ♀ from the F group showed enhanced lipid peroxidation (TBARS) levels, elevated catalase ac- tivity and increased superoxide dismutase protein expression in the whole brain. Neuroinflammation was corroborated by increased pro- tein expression of cyclooxygenase 2. The acquisition of developing milestones was delayed in ♀ from F-NC and F-NF groups. In com- parison with C-NC litter, aggressive social behavior increased while memory retention and nociception were reduced in offspring from F-NC. The ability of recognizing a previously presented stimulus was affected only in ♂ from the F-NC group. These findings suggest that F-induced maternal MetS results in offspring neurodevelopmen- tal delay and neurobehavioral changes probably associated with early brain oxidative stress and inflammation. Interestingly, offspring gender differences were noted
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227135
Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring; Reunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 185-185
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227135
identifier_str_mv Fructose -induced maternal metabolic syndrome leads to cerebral oxidative stress, neuroinflammation and neurobehavioral impairments in offspring; Reunión Conjunta SAIC SAI & FAIC SAFIS 2022; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología; Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 185-185
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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