Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring

Autores
Prado Spalm, Facundo Heber; Cuervo Sanchez, Marie Lucia; Furland, Natalia Edith; Valles, Ana Sofia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Objective: The aim of this work was to determine if mater- nal metabolic syndrome (MetS), induced by a high fruc- tose supplementation, induces long-term neurological and metabolic alterations in offspring. Material & Meth- ods: 2 months old female Wistar rats were fed a standard diet and drunk either tap water alone or supplemented with 20% fructose, for 10 weeks, to induce MetS. Then they were mated with healthy males to generate litters (OC: offspring from control dams n=6; OF: offspring from fructose dams, n=6). So as to analyze only the prena- tal effects of maternal MetS, all the pups were breastfed by control nurse dams, that had access to a standard diet and water ad libitum until weaning. Cognitive and social performance were evaluated between postnatal day (PN) 22 and 90. Animals were sacrificed on PN100 and metabolic parameters were analyzed. Normality of the data was analyzed by Shapiro-Wilk´s test, homosce- dasticity by Bartlett´s test and then parametric (t-test) or non-parametric (Mann-Whitney) tests were performed on the data. Results: The elevated plus maze, the open field and the marble burying tests revealed an increased anx- iety-like phenotype in females OF. On the contrary, the novel object recognition test showed that only the males from the OF group had long-term memory impairment. In the reciprocal social interaction test, both male and female OF presented lower number of social interac- tions, while only females showed significant increments in “socially inactive” behavior. Furthermore, in the Three Chamber Test, only females OF had lower social prefer- ence and social novelty indexes. In regards to metabolic parameters, females OF had increased levels of serum triglycerides and higher visceral fat percentage. Conclu- sions: maternal MetS has long-term adverse effects on the neurological and metabolic status of offspring rats with sexual dimorphism
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentinad de Biología
Asociación Argentina De Farmacología Experimental
Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
Materia
FRUCTOSA
SINDROME METABOLICO MATERNO
NEURODESARROLLO
COMPORTAMIENTO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247553
Acceder
id CONICETDig_b67fe5e7171b6fbcb3bc9976013b8433
oai_identifier_str oai:ri.conicet.gov.ar:11336/247553
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In OffspringPrado Spalm, Facundo HeberCuervo Sanchez, Marie LuciaFurland, Natalia EdithValles, Ana SofiaFRUCTOSASINDROME METABOLICO MATERNONEURODESARROLLOCOMPORTAMIENTOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: The aim of this work was to determine if mater- nal metabolic syndrome (MetS), induced by a high fruc- tose supplementation, induces long-term neurological and metabolic alterations in offspring. Material & Meth- ods: 2 months old female Wistar rats were fed a standard diet and drunk either tap water alone or supplemented with 20% fructose, for 10 weeks, to induce MetS. Then they were mated with healthy males to generate litters (OC: offspring from control dams n=6; OF: offspring from fructose dams, n=6). So as to analyze only the prena- tal effects of maternal MetS, all the pups were breastfed by control nurse dams, that had access to a standard diet and water ad libitum until weaning. Cognitive and social performance were evaluated between postnatal day (PN) 22 and 90. Animals were sacrificed on PN100 and metabolic parameters were analyzed. Normality of the data was analyzed by Shapiro-Wilk´s test, homosce- dasticity by Bartlett´s test and then parametric (t-test) or non-parametric (Mann-Whitney) tests were performed on the data. Results: The elevated plus maze, the open field and the marble burying tests revealed an increased anx- iety-like phenotype in females OF. On the contrary, the novel object recognition test showed that only the males from the OF group had long-term memory impairment. In the reciprocal social interaction test, both male and female OF presented lower number of social interac- tions, while only females showed significant increments in “socially inactive” behavior. Furthermore, in the Three Chamber Test, only females OF had lower social prefer- ence and social novelty indexes. In regards to metabolic parameters, females OF had increased levels of serum triglycerides and higher visceral fat percentage. Conclu- sions: maternal MetS has long-term adverse effects on the neurological and metabolic status of offspring rats with sexual dimorphismFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularSociedad Argentinad de BiologíaAsociación Argentina De Farmacología ExperimentalAsociación Argentina De Ciencia Y Tecnología De Animales De LaboratorioFundación Revista MedicinaLuthy, Isabel AliciaPerez Martinez, Silvina LauraSimonovich, VenturaPinto, Gabriel2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247553Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring; Reunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio; Mar del Plata; Argentina; 2023; 69-690025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:50Zoai:ri.conicet.gov.ar:11336/247553instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:51.306CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
title Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
spellingShingle Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
Prado Spalm, Facundo Heber
FRUCTOSA
SINDROME METABOLICO MATERNO
NEURODESARROLLO
COMPORTAMIENTO
title_short Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
title_full Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
title_fullStr Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
title_full_unstemmed Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
title_sort Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring
dc.creator.none.fl_str_mv Prado Spalm, Facundo Heber
Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author Prado Spalm, Facundo Heber
author_facet Prado Spalm, Facundo Heber
Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author_role author
author2 Cuervo Sanchez, Marie Lucia
Furland, Natalia Edith
Valles, Ana Sofia
author2_role author
author
author
dc.contributor.none.fl_str_mv Luthy, Isabel Alicia
Perez Martinez, Silvina Laura
Simonovich, Ventura
Pinto, Gabriel
dc.subject.none.fl_str_mv FRUCTOSA
SINDROME METABOLICO MATERNO
NEURODESARROLLO
COMPORTAMIENTO
topic FRUCTOSA
SINDROME METABOLICO MATERNO
NEURODESARROLLO
COMPORTAMIENTO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: The aim of this work was to determine if mater- nal metabolic syndrome (MetS), induced by a high fruc- tose supplementation, induces long-term neurological and metabolic alterations in offspring. Material & Meth- ods: 2 months old female Wistar rats were fed a standard diet and drunk either tap water alone or supplemented with 20% fructose, for 10 weeks, to induce MetS. Then they were mated with healthy males to generate litters (OC: offspring from control dams n=6; OF: offspring from fructose dams, n=6). So as to analyze only the prena- tal effects of maternal MetS, all the pups were breastfed by control nurse dams, that had access to a standard diet and water ad libitum until weaning. Cognitive and social performance were evaluated between postnatal day (PN) 22 and 90. Animals were sacrificed on PN100 and metabolic parameters were analyzed. Normality of the data was analyzed by Shapiro-Wilk´s test, homosce- dasticity by Bartlett´s test and then parametric (t-test) or non-parametric (Mann-Whitney) tests were performed on the data. Results: The elevated plus maze, the open field and the marble burying tests revealed an increased anx- iety-like phenotype in females OF. On the contrary, the novel object recognition test showed that only the males from the OF group had long-term memory impairment. In the reciprocal social interaction test, both male and female OF presented lower number of social interac- tions, while only females showed significant increments in “socially inactive” behavior. Furthermore, in the Three Chamber Test, only females OF had lower social prefer- ence and social novelty indexes. In regards to metabolic parameters, females OF had increased levels of serum triglycerides and higher visceral fat percentage. Conclu- sions: maternal MetS has long-term adverse effects on the neurological and metabolic status of offspring rats with sexual dimorphism
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cuervo Sanchez, Marie Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Valles, Ana Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Sociedad Argentinad de Biología
Asociación Argentina De Farmacología Experimental
Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio
description Objective: The aim of this work was to determine if mater- nal metabolic syndrome (MetS), induced by a high fruc- tose supplementation, induces long-term neurological and metabolic alterations in offspring. Material & Meth- ods: 2 months old female Wistar rats were fed a standard diet and drunk either tap water alone or supplemented with 20% fructose, for 10 weeks, to induce MetS. Then they were mated with healthy males to generate litters (OC: offspring from control dams n=6; OF: offspring from fructose dams, n=6). So as to analyze only the prena- tal effects of maternal MetS, all the pups were breastfed by control nurse dams, that had access to a standard diet and water ad libitum until weaning. Cognitive and social performance were evaluated between postnatal day (PN) 22 and 90. Animals were sacrificed on PN100 and metabolic parameters were analyzed. Normality of the data was analyzed by Shapiro-Wilk´s test, homosce- dasticity by Bartlett´s test and then parametric (t-test) or non-parametric (Mann-Whitney) tests were performed on the data. Results: The elevated plus maze, the open field and the marble burying tests revealed an increased anx- iety-like phenotype in females OF. On the contrary, the novel object recognition test showed that only the males from the OF group had long-term memory impairment. In the reciprocal social interaction test, both male and female OF presented lower number of social interac- tions, while only females showed significant increments in “socially inactive” behavior. Furthermore, in the Three Chamber Test, only females OF had lower social prefer- ence and social novelty indexes. In regards to metabolic parameters, females OF had increased levels of serum triglycerides and higher visceral fat percentage. Conclu- sions: maternal MetS has long-term adverse effects on the neurological and metabolic status of offspring rats with sexual dimorphism
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247553
Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring; Reunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio; Mar del Plata; Argentina; 2023; 69-69
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247553
identifier_str_mv Dam’s Fructose Induced-Metabolic Syndrome Promotes Long-Term Neurological Alterations In Offspring; Reunión Conjunta LXVIII Sociedad Argentina De Investigación Clínica, XXV Jornadas Anuales De La Sociedad Argentina De Biología, LV Reunión Anual De La Asociación Argentina De Farmacología Experimental, VIII Reunión Científica Regional De La Asociación Argentina De Ciencia Y Tecnología De Animales De Laboratorio; Mar del Plata; Argentina; 2023; 69-69
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol83-23/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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