Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern...

Autores
Nieves, Elvia Ester; Fleitas, Pedro Emanuel; Juárez, Marisa; Almazán, María Cristina; Flores, Gabriela; Alani, Jimena; Diaz, Ramón; Martos, Jorge; Cajal, Pamela; Cimino, Rubén Oscar; Krolewiecki, Alejandro Javier
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Soil-transmitted helminths (STH) are a significant public health problem in impoverished communitiesof tropical and subtropical areas. Improved diagnostic methods are crucial for NeglectedTropical Diseases programs, particularly for S. stercoralis, as traditional methods are inadequate.Thus, it is important to identify the most accurate and efficient methods for the diagnosis of STH.We performed a retrospective study analyzing laboratory data at the Instituto de Investigacionesde Enfermedades Tropicales from 2010 to 2019. The study included data from outpatientsreferred for stool analysis and public health interventions from urban and rural communities innorthern Salta province, Argentina. Samples were included in this analysis if processed throughsedimentation/concentration, Baermann, Harada-Mori and McMaster´s, with a subgroup that alsoincluded Agar plate culture method (APC). Sensitivity was calculated against a compositereference standard. Of the 5625 samples collected, 944 qualified for this analysis, with a prevalenceof 11.14% for A. lumbricoides, 8.16% for hookworm, 1.38% for T. trichiura, and 6.36% forS. stercoralis. The sedimentation/concentration method was the most sensitive for A. lumbricoides(96%), compared to the McMaster method, with a sensitivity of 62%. Similarly, for hookworms,sedimentation/concentration was more sensitive than McMaster´s, Harada-Mori, and Baermannwith sensitivities of 87%, 70%, 43%, and 13%, respectively. Most of these infections were of light intensity. For S. stercoralis, Baermann and sedimentation/concentration methods were the mostsensitive, with 70% and 62% respectively, while Harada-Mori was the least sensitive. In a subsetof 389 samples also analyzed by the APC, Baermann was more sensitive than APC for detectingS. stercoralis, and both methods were superior to Harada-Mori. Parasitological methods, mostlywhen used combined, offer adequate opportunities for the diagnosis of STH in clinical and publichealth laboratories. The incorporation of S. stercoralis into the control strategies of the WorldHealth Organization requires rethinking the current diagnostic approach used for surveys. Withsedimentation/concentration and Baermann appearing as the most sensitive methods for thisspecies. Further studies, including implementation assessments, should help in identifying themost adequate and feasible all-STH diagnostic approach.
Fil: Nieves, Elvia Ester. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fleitas, Pedro Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Salud Global de Barcelona; España
Fil: Juárez, Marisa. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Almazán, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Flores, Gabriela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Alani, Jimena. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Diaz, Ramón. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Martos, Jorge. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
SOIL TRANSMITTED HELMINTHS
STRONGYLOIDES STERCORALIS
BAERMANN
DIAGNOSTICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243641
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational studyNieves, Elvia EsterFleitas, Pedro EmanuelJuárez, MarisaAlmazán, María CristinaFlores, GabrielaAlani, JimenaDiaz, RamónMartos, JorgeCajal, PamelaCimino, Rubén OscarKrolewiecki, Alejandro JavierSOIL TRANSMITTED HELMINTHSSTRONGYLOIDES STERCORALISBAERMANNDIAGNOSTICShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Soil-transmitted helminths (STH) are a significant public health problem in impoverished communitiesof tropical and subtropical areas. Improved diagnostic methods are crucial for NeglectedTropical Diseases programs, particularly for S. stercoralis, as traditional methods are inadequate.Thus, it is important to identify the most accurate and efficient methods for the diagnosis of STH.We performed a retrospective study analyzing laboratory data at the Instituto de Investigacionesde Enfermedades Tropicales from 2010 to 2019. The study included data from outpatientsreferred for stool analysis and public health interventions from urban and rural communities innorthern Salta province, Argentina. Samples were included in this analysis if processed throughsedimentation/concentration, Baermann, Harada-Mori and McMaster´s, with a subgroup that alsoincluded Agar plate culture method (APC). Sensitivity was calculated against a compositereference standard. Of the 5625 samples collected, 944 qualified for this analysis, with a prevalenceof 11.14% for A. lumbricoides, 8.16% for hookworm, 1.38% for T. trichiura, and 6.36% forS. stercoralis. The sedimentation/concentration method was the most sensitive for A. lumbricoides(96%), compared to the McMaster method, with a sensitivity of 62%. Similarly, for hookworms,sedimentation/concentration was more sensitive than McMaster´s, Harada-Mori, and Baermannwith sensitivities of 87%, 70%, 43%, and 13%, respectively. Most of these infections were of light intensity. For S. stercoralis, Baermann and sedimentation/concentration methods were the mostsensitive, with 70% and 62% respectively, while Harada-Mori was the least sensitive. In a subsetof 389 samples also analyzed by the APC, Baermann was more sensitive than APC for detectingS. stercoralis, and both methods were superior to Harada-Mori. Parasitological methods, mostlywhen used combined, offer adequate opportunities for the diagnosis of STH in clinical and publichealth laboratories. The incorporation of S. stercoralis into the control strategies of the WorldHealth Organization requires rethinking the current diagnostic approach used for surveys. Withsedimentation/concentration and Baermann appearing as the most sensitive methods for thisspecies. Further studies, including implementation assessments, should help in identifying themost adequate and feasible all-STH diagnostic approach.Fil: Nieves, Elvia Ester. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fleitas, Pedro Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Salud Global de Barcelona; EspañaFil: Juárez, Marisa. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Almazán, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Flores, Gabriela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Alani, Jimena. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Diaz, Ramón. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Martos, Jorge. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/243641Nieves, Elvia Ester; Fleitas, Pedro Emanuel; Juárez, Marisa; Almazán, María Cristina; Flores, Gabriela; et al.; Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study; Elsevier; Parasite Epidemiology and Control; 26; 8-2024; 1-72405-6731CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2405673124000345info:eu-repo/semantics/altIdentifier/doi/10.1016/j.parepi.2024.e00370info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:40Zoai:ri.conicet.gov.ar:11336/243641instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:41.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
title Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
spellingShingle Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
Nieves, Elvia Ester
SOIL TRANSMITTED HELMINTHS
STRONGYLOIDES STERCORALIS
BAERMANN
DIAGNOSTICS
title_short Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
title_full Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
title_fullStr Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
title_full_unstemmed Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
title_sort Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study
dc.creator.none.fl_str_mv Nieves, Elvia Ester
Fleitas, Pedro Emanuel
Juárez, Marisa
Almazán, María Cristina
Flores, Gabriela
Alani, Jimena
Diaz, Ramón
Martos, Jorge
Cajal, Pamela
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
author Nieves, Elvia Ester
author_facet Nieves, Elvia Ester
Fleitas, Pedro Emanuel
Juárez, Marisa
Almazán, María Cristina
Flores, Gabriela
Alani, Jimena
Diaz, Ramón
Martos, Jorge
Cajal, Pamela
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
author_role author
author2 Fleitas, Pedro Emanuel
Juárez, Marisa
Almazán, María Cristina
Flores, Gabriela
Alani, Jimena
Diaz, Ramón
Martos, Jorge
Cajal, Pamela
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SOIL TRANSMITTED HELMINTHS
STRONGYLOIDES STERCORALIS
BAERMANN
DIAGNOSTICS
topic SOIL TRANSMITTED HELMINTHS
STRONGYLOIDES STERCORALIS
BAERMANN
DIAGNOSTICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Soil-transmitted helminths (STH) are a significant public health problem in impoverished communitiesof tropical and subtropical areas. Improved diagnostic methods are crucial for NeglectedTropical Diseases programs, particularly for S. stercoralis, as traditional methods are inadequate.Thus, it is important to identify the most accurate and efficient methods for the diagnosis of STH.We performed a retrospective study analyzing laboratory data at the Instituto de Investigacionesde Enfermedades Tropicales from 2010 to 2019. The study included data from outpatientsreferred for stool analysis and public health interventions from urban and rural communities innorthern Salta province, Argentina. Samples were included in this analysis if processed throughsedimentation/concentration, Baermann, Harada-Mori and McMaster´s, with a subgroup that alsoincluded Agar plate culture method (APC). Sensitivity was calculated against a compositereference standard. Of the 5625 samples collected, 944 qualified for this analysis, with a prevalenceof 11.14% for A. lumbricoides, 8.16% for hookworm, 1.38% for T. trichiura, and 6.36% forS. stercoralis. The sedimentation/concentration method was the most sensitive for A. lumbricoides(96%), compared to the McMaster method, with a sensitivity of 62%. Similarly, for hookworms,sedimentation/concentration was more sensitive than McMaster´s, Harada-Mori, and Baermannwith sensitivities of 87%, 70%, 43%, and 13%, respectively. Most of these infections were of light intensity. For S. stercoralis, Baermann and sedimentation/concentration methods were the mostsensitive, with 70% and 62% respectively, while Harada-Mori was the least sensitive. In a subsetof 389 samples also analyzed by the APC, Baermann was more sensitive than APC for detectingS. stercoralis, and both methods were superior to Harada-Mori. Parasitological methods, mostlywhen used combined, offer adequate opportunities for the diagnosis of STH in clinical and publichealth laboratories. The incorporation of S. stercoralis into the control strategies of the WorldHealth Organization requires rethinking the current diagnostic approach used for surveys. Withsedimentation/concentration and Baermann appearing as the most sensitive methods for thisspecies. Further studies, including implementation assessments, should help in identifying themost adequate and feasible all-STH diagnostic approach.
Fil: Nieves, Elvia Ester. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fleitas, Pedro Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Salud Global de Barcelona; España
Fil: Juárez, Marisa. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Almazán, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Flores, Gabriela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Alani, Jimena. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Diaz, Ramón. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Martos, Jorge. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Soil-transmitted helminths (STH) are a significant public health problem in impoverished communitiesof tropical and subtropical areas. Improved diagnostic methods are crucial for NeglectedTropical Diseases programs, particularly for S. stercoralis, as traditional methods are inadequate.Thus, it is important to identify the most accurate and efficient methods for the diagnosis of STH.We performed a retrospective study analyzing laboratory data at the Instituto de Investigacionesde Enfermedades Tropicales from 2010 to 2019. The study included data from outpatientsreferred for stool analysis and public health interventions from urban and rural communities innorthern Salta province, Argentina. Samples were included in this analysis if processed throughsedimentation/concentration, Baermann, Harada-Mori and McMaster´s, with a subgroup that alsoincluded Agar plate culture method (APC). Sensitivity was calculated against a compositereference standard. Of the 5625 samples collected, 944 qualified for this analysis, with a prevalenceof 11.14% for A. lumbricoides, 8.16% for hookworm, 1.38% for T. trichiura, and 6.36% forS. stercoralis. The sedimentation/concentration method was the most sensitive for A. lumbricoides(96%), compared to the McMaster method, with a sensitivity of 62%. Similarly, for hookworms,sedimentation/concentration was more sensitive than McMaster´s, Harada-Mori, and Baermannwith sensitivities of 87%, 70%, 43%, and 13%, respectively. Most of these infections were of light intensity. For S. stercoralis, Baermann and sedimentation/concentration methods were the mostsensitive, with 70% and 62% respectively, while Harada-Mori was the least sensitive. In a subsetof 389 samples also analyzed by the APC, Baermann was more sensitive than APC for detectingS. stercoralis, and both methods were superior to Harada-Mori. Parasitological methods, mostlywhen used combined, offer adequate opportunities for the diagnosis of STH in clinical and publichealth laboratories. The incorporation of S. stercoralis into the control strategies of the WorldHealth Organization requires rethinking the current diagnostic approach used for surveys. Withsedimentation/concentration and Baermann appearing as the most sensitive methods for thisspecies. Further studies, including implementation assessments, should help in identifying themost adequate and feasible all-STH diagnostic approach.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243641
Nieves, Elvia Ester; Fleitas, Pedro Emanuel; Juárez, Marisa; Almazán, María Cristina; Flores, Gabriela; et al.; Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study; Elsevier; Parasite Epidemiology and Control; 26; 8-2024; 1-7
2405-6731
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243641
identifier_str_mv Nieves, Elvia Ester; Fleitas, Pedro Emanuel; Juárez, Marisa; Almazán, María Cristina; Flores, Gabriela; et al.; Comparison of parasitological methods for the identification of soil-transmitted helminths, including Strongyloides stercoralis, in a regional reference laboratory in northwestern Argentina: An observational study; Elsevier; Parasite Epidemiology and Control; 26; 8-2024; 1-7
2405-6731
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.parepi.2024.e00370
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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reponame_str CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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