High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up
- Autores
- Amor, Aranzazu; Rodriguez, Esperanza; Saugar, José M.; Arroyo, Ana; López-Quintana, Beatriz; Abera, Bayeh; Yimer, Mulat; Yizengaw, Endalew; Zewdie, Derejew; Ayehubizu, Zimman; Hailu, Tadesse; Mulu, Wondemagegn; Echazú, Adriana; Krolewieki, Alejandro J.; Aparicio, Pilar; Herrador, Zaida; Anegagrie, Melaku; Benito, Agustín
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Soil-transmitted helminthiases (hookworms, Ascaris lumbricoides and Trichuris trichiura) are extremely prevalent in school-aged children living in poor sanitary conditions. Recent epidemiological data suggest that Strongyloides stercoralis is highly unreported. However, accurate data are essential for conducting interventions aimed at introducing control and elimination programmes. Methods: We conducted a cross-sectional survey of 396 randomly selected school-aged children in Amhara region in rural area in north-western Ethiopia, to assess the prevalence of S. stercoralis and other intestinal helminths. We examined stools using three techniques: conventional stool concentration; and two S. stercoralis-specific methods, i.e. the Baermann technique and polymerase chain reaction. The diagnostic accuracy of these three methods was then compared. Results: There was an overall prevalence of helminths of 77.5%, with distribution differing according to school setting. Soil-transmitted helminths were recorded in 69.2%. Prevalence of S. stercoralis and hookworm infection was 20.7 and 54.5%, respectively, and co-infection was detected in 16.3% of cases. Schistosoma mansoni had a prevalence of 15.7%. Prevalence of S. stercoralis was shown 3.5% by the conventional method, 12.1% by the Baermann method, and 13.4% by PCR, which thus proved to be the most sensitive. Conclusions: Our results suggest that S. stercoralis could be overlooked and neglected in Ethiopia, if studies of soil-transmitted helminths rely on conventional diagnostic techniques alone. A combination of molecular and stool microscopy techniques yields a significantly higher prevalence. In view of the fact that current control policies for triggering drug administration are based on parasite prevalence levels, a comprehensive diagnostic approach should instead be applied to ensure comprehensive control of helminth infections.
Fil: Amor, Aranzazu. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Rodriguez, Esperanza. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Saugar, José M.. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Arroyo, Ana. Hospital Universitario la Paz - Carlos III; España
Fil: López-Quintana, Beatriz. Hospital Universitario la Paz - Carlos III; España
Fil: Abera, Bayeh. Bahir Dar University; Etiopía
Fil: Yimer, Mulat. Bahir Dar University; Etiopía
Fil: Yizengaw, Endalew. Bahir Dar University; Etiopía
Fil: Zewdie, Derejew. Bahir Dar University; Etiopía
Fil: Ayehubizu, Zimman. Bahir Dar University; Etiopía
Fil: Hailu, Tadesse. Bahir Dar University; Etiopía
Fil: Mulu, Wondemagegn. Bahir Dar University; Etiopía
Fil: Echazú, Adriana. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewieki, Alejandro J.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aparicio, Pilar. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Herrador, Zaida. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Anegagrie, Melaku. Universidad Carlos III de Madrid. Instituto de Salud; España
Fil: Benito, Agustín. Universidad Carlos III de Madrid. Instituto de Salud; España - Materia
-
BAERMANN TECHNIQUE
ETHIOPIA
MOLECULAR TECHNIQUES
NEGLECTED
SOIL-TRANSMITTED HELMINTHS
STRONGYLOIDES STERCORALIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/37498
Ver los metadatos del registro completo
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High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-upAmor, AranzazuRodriguez, EsperanzaSaugar, José M.Arroyo, AnaLópez-Quintana, BeatrizAbera, BayehYimer, MulatYizengaw, EndalewZewdie, DerejewAyehubizu, ZimmanHailu, TadesseMulu, WondemagegnEchazú, AdrianaKrolewieki, Alejandro J.Aparicio, PilarHerrador, ZaidaAnegagrie, MelakuBenito, AgustínBAERMANN TECHNIQUEETHIOPIAMOLECULAR TECHNIQUESNEGLECTEDSOIL-TRANSMITTED HELMINTHSSTRONGYLOIDES STERCORALIShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Soil-transmitted helminthiases (hookworms, Ascaris lumbricoides and Trichuris trichiura) are extremely prevalent in school-aged children living in poor sanitary conditions. Recent epidemiological data suggest that Strongyloides stercoralis is highly unreported. However, accurate data are essential for conducting interventions aimed at introducing control and elimination programmes. Methods: We conducted a cross-sectional survey of 396 randomly selected school-aged children in Amhara region in rural area in north-western Ethiopia, to assess the prevalence of S. stercoralis and other intestinal helminths. We examined stools using three techniques: conventional stool concentration; and two S. stercoralis-specific methods, i.e. the Baermann technique and polymerase chain reaction. The diagnostic accuracy of these three methods was then compared. Results: There was an overall prevalence of helminths of 77.5%, with distribution differing according to school setting. Soil-transmitted helminths were recorded in 69.2%. Prevalence of S. stercoralis and hookworm infection was 20.7 and 54.5%, respectively, and co-infection was detected in 16.3% of cases. Schistosoma mansoni had a prevalence of 15.7%. Prevalence of S. stercoralis was shown 3.5% by the conventional method, 12.1% by the Baermann method, and 13.4% by PCR, which thus proved to be the most sensitive. Conclusions: Our results suggest that S. stercoralis could be overlooked and neglected in Ethiopia, if studies of soil-transmitted helminths rely on conventional diagnostic techniques alone. A combination of molecular and stool microscopy techniques yields a significantly higher prevalence. In view of the fact that current control policies for triggering drug administration are based on parasite prevalence levels, a comprehensive diagnostic approach should instead be applied to ensure comprehensive control of helminth infections.Fil: Amor, Aranzazu. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Rodriguez, Esperanza. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Saugar, José M.. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Arroyo, Ana. Hospital Universitario la Paz - Carlos III; EspañaFil: López-Quintana, Beatriz. Hospital Universitario la Paz - Carlos III; EspañaFil: Abera, Bayeh. Bahir Dar University; EtiopíaFil: Yimer, Mulat. Bahir Dar University; EtiopíaFil: Yizengaw, Endalew. Bahir Dar University; EtiopíaFil: Zewdie, Derejew. Bahir Dar University; EtiopíaFil: Ayehubizu, Zimman. Bahir Dar University; EtiopíaFil: Hailu, Tadesse. Bahir Dar University; EtiopíaFil: Mulu, Wondemagegn. Bahir Dar University; EtiopíaFil: Echazú, Adriana. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krolewieki, Alejandro J.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aparicio, Pilar. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Herrador, Zaida. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Anegagrie, Melaku. Universidad Carlos III de Madrid. Instituto de Salud; EspañaFil: Benito, Agustín. Universidad Carlos III de Madrid. Instituto de Salud; EspañaBioMed Central2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37498Amor, Aranzazu; Rodriguez, Esperanza; Saugar, José M.; Arroyo, Ana; López-Quintana, Beatriz; et al.; High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up; BioMed Central; Parasites and Vectors; 9; 1; 1-2016; 1-91756-3305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-016-1912-8info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1912-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:47Zoai:ri.conicet.gov.ar:11336/37498instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:47.914CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| title |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| spellingShingle |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up Amor, Aranzazu BAERMANN TECHNIQUE ETHIOPIA MOLECULAR TECHNIQUES NEGLECTED SOIL-TRANSMITTED HELMINTHS STRONGYLOIDES STERCORALIS |
| title_short |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| title_full |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| title_fullStr |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| title_full_unstemmed |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| title_sort |
High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up |
| dc.creator.none.fl_str_mv |
Amor, Aranzazu Rodriguez, Esperanza Saugar, José M. Arroyo, Ana López-Quintana, Beatriz Abera, Bayeh Yimer, Mulat Yizengaw, Endalew Zewdie, Derejew Ayehubizu, Zimman Hailu, Tadesse Mulu, Wondemagegn Echazú, Adriana Krolewieki, Alejandro J. Aparicio, Pilar Herrador, Zaida Anegagrie, Melaku Benito, Agustín |
| author |
Amor, Aranzazu |
| author_facet |
Amor, Aranzazu Rodriguez, Esperanza Saugar, José M. Arroyo, Ana López-Quintana, Beatriz Abera, Bayeh Yimer, Mulat Yizengaw, Endalew Zewdie, Derejew Ayehubizu, Zimman Hailu, Tadesse Mulu, Wondemagegn Echazú, Adriana Krolewieki, Alejandro J. Aparicio, Pilar Herrador, Zaida Anegagrie, Melaku Benito, Agustín |
| author_role |
author |
| author2 |
Rodriguez, Esperanza Saugar, José M. Arroyo, Ana López-Quintana, Beatriz Abera, Bayeh Yimer, Mulat Yizengaw, Endalew Zewdie, Derejew Ayehubizu, Zimman Hailu, Tadesse Mulu, Wondemagegn Echazú, Adriana Krolewieki, Alejandro J. Aparicio, Pilar Herrador, Zaida Anegagrie, Melaku Benito, Agustín |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
BAERMANN TECHNIQUE ETHIOPIA MOLECULAR TECHNIQUES NEGLECTED SOIL-TRANSMITTED HELMINTHS STRONGYLOIDES STERCORALIS |
| topic |
BAERMANN TECHNIQUE ETHIOPIA MOLECULAR TECHNIQUES NEGLECTED SOIL-TRANSMITTED HELMINTHS STRONGYLOIDES STERCORALIS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Background: Soil-transmitted helminthiases (hookworms, Ascaris lumbricoides and Trichuris trichiura) are extremely prevalent in school-aged children living in poor sanitary conditions. Recent epidemiological data suggest that Strongyloides stercoralis is highly unreported. However, accurate data are essential for conducting interventions aimed at introducing control and elimination programmes. Methods: We conducted a cross-sectional survey of 396 randomly selected school-aged children in Amhara region in rural area in north-western Ethiopia, to assess the prevalence of S. stercoralis and other intestinal helminths. We examined stools using three techniques: conventional stool concentration; and two S. stercoralis-specific methods, i.e. the Baermann technique and polymerase chain reaction. The diagnostic accuracy of these three methods was then compared. Results: There was an overall prevalence of helminths of 77.5%, with distribution differing according to school setting. Soil-transmitted helminths were recorded in 69.2%. Prevalence of S. stercoralis and hookworm infection was 20.7 and 54.5%, respectively, and co-infection was detected in 16.3% of cases. Schistosoma mansoni had a prevalence of 15.7%. Prevalence of S. stercoralis was shown 3.5% by the conventional method, 12.1% by the Baermann method, and 13.4% by PCR, which thus proved to be the most sensitive. Conclusions: Our results suggest that S. stercoralis could be overlooked and neglected in Ethiopia, if studies of soil-transmitted helminths rely on conventional diagnostic techniques alone. A combination of molecular and stool microscopy techniques yields a significantly higher prevalence. In view of the fact that current control policies for triggering drug administration are based on parasite prevalence levels, a comprehensive diagnostic approach should instead be applied to ensure comprehensive control of helminth infections. Fil: Amor, Aranzazu. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Rodriguez, Esperanza. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Saugar, José M.. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Arroyo, Ana. Hospital Universitario la Paz - Carlos III; España Fil: López-Quintana, Beatriz. Hospital Universitario la Paz - Carlos III; España Fil: Abera, Bayeh. Bahir Dar University; Etiopía Fil: Yimer, Mulat. Bahir Dar University; Etiopía Fil: Yizengaw, Endalew. Bahir Dar University; Etiopía Fil: Zewdie, Derejew. Bahir Dar University; Etiopía Fil: Ayehubizu, Zimman. Bahir Dar University; Etiopía Fil: Hailu, Tadesse. Bahir Dar University; Etiopía Fil: Mulu, Wondemagegn. Bahir Dar University; Etiopía Fil: Echazú, Adriana. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Krolewieki, Alejandro J.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aparicio, Pilar. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Herrador, Zaida. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Anegagrie, Melaku. Universidad Carlos III de Madrid. Instituto de Salud; España Fil: Benito, Agustín. Universidad Carlos III de Madrid. Instituto de Salud; España |
| description |
Background: Soil-transmitted helminthiases (hookworms, Ascaris lumbricoides and Trichuris trichiura) are extremely prevalent in school-aged children living in poor sanitary conditions. Recent epidemiological data suggest that Strongyloides stercoralis is highly unreported. However, accurate data are essential for conducting interventions aimed at introducing control and elimination programmes. Methods: We conducted a cross-sectional survey of 396 randomly selected school-aged children in Amhara region in rural area in north-western Ethiopia, to assess the prevalence of S. stercoralis and other intestinal helminths. We examined stools using three techniques: conventional stool concentration; and two S. stercoralis-specific methods, i.e. the Baermann technique and polymerase chain reaction. The diagnostic accuracy of these three methods was then compared. Results: There was an overall prevalence of helminths of 77.5%, with distribution differing according to school setting. Soil-transmitted helminths were recorded in 69.2%. Prevalence of S. stercoralis and hookworm infection was 20.7 and 54.5%, respectively, and co-infection was detected in 16.3% of cases. Schistosoma mansoni had a prevalence of 15.7%. Prevalence of S. stercoralis was shown 3.5% by the conventional method, 12.1% by the Baermann method, and 13.4% by PCR, which thus proved to be the most sensitive. Conclusions: Our results suggest that S. stercoralis could be overlooked and neglected in Ethiopia, if studies of soil-transmitted helminths rely on conventional diagnostic techniques alone. A combination of molecular and stool microscopy techniques yields a significantly higher prevalence. In view of the fact that current control policies for triggering drug administration are based on parasite prevalence levels, a comprehensive diagnostic approach should instead be applied to ensure comprehensive control of helminth infections. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/37498 Amor, Aranzazu; Rodriguez, Esperanza; Saugar, José M.; Arroyo, Ana; López-Quintana, Beatriz; et al.; High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up; BioMed Central; Parasites and Vectors; 9; 1; 1-2016; 1-9 1756-3305 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/37498 |
| identifier_str_mv |
Amor, Aranzazu; Rodriguez, Esperanza; Saugar, José M.; Arroyo, Ana; López-Quintana, Beatriz; et al.; High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up; BioMed Central; Parasites and Vectors; 9; 1; 1-2016; 1-9 1756-3305 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-016-1912-8 info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1912-8 |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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application/pdf application/pdf |
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BioMed Central |
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BioMed Central |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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