Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection

Autores
Bisoffi, Zeno; Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Rubén Oscar; Krolewiecki, Alejandro Javier; Albonico, Marco; Gobbo, Maria; Bonafini, Stefania; Angheben, Andrea; Requena-Mendez, Ana; Muñoz, José; Nutman, Thomas B.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background:The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard.Methods:The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests).Results:According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity.Conclusions:NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.
Fil: Bisoffi, Zeno. Sacro Cuore Hospital; Italia
Fil: Buonfrate, Dora. Sacro Cuore Hospital; Italia
Fil: Sequi, Marco. Istituto Di Ricerche Farmacologiche Mario Negri; Italia
Fil: Mejia, Rojelio. National Institute Of Allergy And Infectious Diseases; Estados Unidos
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albonico, Marco. Sacro Cuore Hospital; Italia
Fil: Gobbo, Maria. Sacro Cuore Hospital; Italia
Fil: Bonafini, Stefania. Sacro Cuore Hospital; Italia
Fil: Angheben, Andrea. Sacro Cuore Hospital; Italia
Fil: Requena-Mendez, Ana. Universidad de Barcelona; España
Fil: Muñoz, José. Universidad de Barcelona; España
Fil: Nutman, Thomas B.. National Institute Of Allergy And Infectious Diseases; Estados Unidos
Materia
Strongyloides Stercoralis
Serology
Diagnostics
Lips
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37122

id CONICETDig_797db583b3050a2273efeaf75f204702
oai_identifier_str oai:ri.conicet.gov.ar:11336/37122
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis InfectionBisoffi, ZenoBuonfrate, DoraSequi, MarcoMejia, RojelioCimino, Rubén OscarKrolewiecki, Alejandro JavierAlbonico, MarcoGobbo, MariaBonafini, StefaniaAngheben, AndreaRequena-Mendez, AnaMuñoz, JoséNutman, Thomas B.Strongyloides StercoralisSerologyDiagnosticsLipshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background:The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard.Methods:The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests).Results:According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity.Conclusions:NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.Fil: Bisoffi, Zeno. Sacro Cuore Hospital; ItaliaFil: Buonfrate, Dora. Sacro Cuore Hospital; ItaliaFil: Sequi, Marco. Istituto Di Ricerche Farmacologiche Mario Negri; ItaliaFil: Mejia, Rojelio. National Institute Of Allergy And Infectious Diseases; Estados UnidosFil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albonico, Marco. Sacro Cuore Hospital; ItaliaFil: Gobbo, Maria. Sacro Cuore Hospital; ItaliaFil: Bonafini, Stefania. Sacro Cuore Hospital; ItaliaFil: Angheben, Andrea. Sacro Cuore Hospital; ItaliaFil: Requena-Mendez, Ana. Universidad de Barcelona; EspañaFil: Muñoz, José. Universidad de Barcelona; EspañaFil: Nutman, Thomas B.. National Institute Of Allergy And Infectious Diseases; Estados UnidosPublic Library of Science2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37122Bisoffi, Zeno; Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Rubén Oscar; et al.; Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 1-8; e26401935-2735CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002640info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002640info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:54Zoai:ri.conicet.gov.ar:11336/37122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:54.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
title Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
spellingShingle Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
Bisoffi, Zeno
Strongyloides Stercoralis
Serology
Diagnostics
Lips
title_short Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
title_full Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
title_fullStr Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
title_full_unstemmed Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
title_sort Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection
dc.creator.none.fl_str_mv Bisoffi, Zeno
Buonfrate, Dora
Sequi, Marco
Mejia, Rojelio
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Gobbo, Maria
Bonafini, Stefania
Angheben, Andrea
Requena-Mendez, Ana
Muñoz, José
Nutman, Thomas B.
author Bisoffi, Zeno
author_facet Bisoffi, Zeno
Buonfrate, Dora
Sequi, Marco
Mejia, Rojelio
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Gobbo, Maria
Bonafini, Stefania
Angheben, Andrea
Requena-Mendez, Ana
Muñoz, José
Nutman, Thomas B.
author_role author
author2 Buonfrate, Dora
Sequi, Marco
Mejia, Rojelio
Cimino, Rubén Oscar
Krolewiecki, Alejandro Javier
Albonico, Marco
Gobbo, Maria
Bonafini, Stefania
Angheben, Andrea
Requena-Mendez, Ana
Muñoz, José
Nutman, Thomas B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Strongyloides Stercoralis
Serology
Diagnostics
Lips
topic Strongyloides Stercoralis
Serology
Diagnostics
Lips
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background:The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard.Methods:The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests).Results:According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity.Conclusions:NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.
Fil: Bisoffi, Zeno. Sacro Cuore Hospital; Italia
Fil: Buonfrate, Dora. Sacro Cuore Hospital; Italia
Fil: Sequi, Marco. Istituto Di Ricerche Farmacologiche Mario Negri; Italia
Fil: Mejia, Rojelio. National Institute Of Allergy And Infectious Diseases; Estados Unidos
Fil: Cimino, Rubén Oscar. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albonico, Marco. Sacro Cuore Hospital; Italia
Fil: Gobbo, Maria. Sacro Cuore Hospital; Italia
Fil: Bonafini, Stefania. Sacro Cuore Hospital; Italia
Fil: Angheben, Andrea. Sacro Cuore Hospital; Italia
Fil: Requena-Mendez, Ana. Universidad de Barcelona; España
Fil: Muñoz, José. Universidad de Barcelona; España
Fil: Nutman, Thomas B.. National Institute Of Allergy And Infectious Diseases; Estados Unidos
description Background:The diagnosis of Strongyloides stercoralis (S. stercoralis) infection is hampered by the suboptimal sensitivity of fecal-based tests. Serological methods are believed to be more sensitive, although assessing their accuracy is difficult because of the lack of sensitivity of a fecal-based reference ("gold") standard.Methods:The sensitivity and specificity of 5 serologic tests for S. stercoralis (in-house IFAT, NIE-ELISA and NIE-LIPS and the commercially available Bordier-ELISA and IVD-ELISA) were assessed on 399 cryopreserved serum samples. Accuracy was measured using fecal results as the primary reference standard, but also using a composite reference standard (based on a combination of tests).Results:According to the latter standard, the most sensitive test was IFAT, with 94.6% sensitivity (91.2-96.9), followed by IVD-ELISA (92.3%, 87.7-96.9). The most specific test was NIE-LIPS, with specificity 99.6% (98.9-100), followed by IVD-ELISA (97.4%, 95.5-99.3). NIE-LIPS did not cross-react with any of the specimens from subjects with other parasitic infections. NIE-LIPS and the two commercial ELISAs approach 100% specificity at a cut off level that maintains ≥70% sensitivity.Conclusions:NIE-LIPS is the most accurate serologic test for the diagnosis of S. stercoralis infection. IFAT and each of the ELISA tests are sufficiently accurate, above a given cut off, for diagnosis, prevalence studies and inclusion in clinical trials.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37122
Bisoffi, Zeno; Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Rubén Oscar; et al.; Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 1-8; e2640
1935-2735
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37122
identifier_str_mv Bisoffi, Zeno; Buonfrate, Dora; Sequi, Marco; Mejia, Rojelio; Cimino, Rubén Oscar; et al.; Diagnostic Accuracy of Five Serologic Tests for Strongyloides stercoralis Infection; Public Library of Science; Neglected Tropical Diseases; 8; 1; 1-2014; 1-8; e2640
1935-2735
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0002640
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002640
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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