Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration

Autores
Andersen, Natalia Denise; Veuthey, Tania Vanesa; Fariña, Milagros; Silbestri, Gustavo Fabián; Rayes, Diego Hernán; de Rosa, Maria Jose
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In this study, we aim to evaluate the role of imidazolium salts as antioxidant and antiaging agents. We synthesized imidazolium salts and use the nematode C. elegans to performed a screening to analyze their ability to improve oxidative stress (OS) resistance. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl)imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to evaluate MSI role in neurodegenerative models, and, ii) to describe the mechanism of action underlying its protective effects. There is a theory that links OS to aging and neurodegeneration. To evaluate MSI neuroprotection, we used C. elegans models of neurodegenerative diseases. As an Alzheimer disease (AD) model, we used a strain that expresses Aβ1-42 in muscle and shows age-dependent protein aggregation and paralysis. Our results show that MSI delays paralysis in this strain. Since mean lifespan is preserved in wild-type worms exposed to MSI, anti-aging effects of MSI in AD model seems to be dependent on its antiproteotoxic role. To gain further insight into its role in other neurotoxic models, we evaluate mobility as an indicator of healthspan in Huntington disease (HD) and Parkinson disease (PD) models. We found that MSI ameliorates mobility rate decline in these proteotoxic models of neurodegenerative diseases. As a first approach to delineate its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. We found that MSI stress protection was not dependent on DAF16. Therefore, other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. Additional research is needed to underpin the mechanism responsible for MSI’s protective role and to confirm if these effects can be extrapolated to other neurodegenerative scenarios.
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Fariña, Milagros. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
2nd Latin American Worm Meeting
Rosario
Argentina
Latin American Worm Meeting
Materia
C ELEGANS
AGING
NEURODEGENERATION
IMIDAZOLIUM SALTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217143
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protective roles of imidazolium salts in C. elegans models of stress and neurodegenerationAndersen, Natalia DeniseVeuthey, Tania VanesaFariña, MilagrosSilbestri, Gustavo FabiánRayes, Diego Hernánde Rosa, Maria JoseC ELEGANSAGINGNEURODEGENERATIONIMIDAZOLIUM SALTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, we aim to evaluate the role of imidazolium salts as antioxidant and antiaging agents. We synthesized imidazolium salts and use the nematode C. elegans to performed a screening to analyze their ability to improve oxidative stress (OS) resistance. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl)imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to evaluate MSI role in neurodegenerative models, and, ii) to describe the mechanism of action underlying its protective effects. There is a theory that links OS to aging and neurodegeneration. To evaluate MSI neuroprotection, we used C. elegans models of neurodegenerative diseases. As an Alzheimer disease (AD) model, we used a strain that expresses Aβ1-42 in muscle and shows age-dependent protein aggregation and paralysis. Our results show that MSI delays paralysis in this strain. Since mean lifespan is preserved in wild-type worms exposed to MSI, anti-aging effects of MSI in AD model seems to be dependent on its antiproteotoxic role. To gain further insight into its role in other neurotoxic models, we evaluate mobility as an indicator of healthspan in Huntington disease (HD) and Parkinson disease (PD) models. We found that MSI ameliorates mobility rate decline in these proteotoxic models of neurodegenerative diseases. As a first approach to delineate its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. We found that MSI stress protection was not dependent on DAF16. Therefore, other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. Additional research is needed to underpin the mechanism responsible for MSI’s protective role and to confirm if these effects can be extrapolated to other neurodegenerative scenarios.Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Fariña, Milagros. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina2nd Latin American Worm MeetingRosarioArgentinaLatin American Worm MeetingUniversidad Nacional de Rosario2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217143Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration; 2nd Latin American Worm Meeting; Rosario; Argentina; 2020; 40-40CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://lawm2020.webnode.es/abstract-submission/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:00Zoai:ri.conicet.gov.ar:11336/217143instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:00.742CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
title Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
spellingShingle Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
Andersen, Natalia Denise
C ELEGANS
AGING
NEURODEGENERATION
IMIDAZOLIUM SALTS
title_short Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
title_full Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
title_fullStr Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
title_full_unstemmed Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
title_sort Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration
dc.creator.none.fl_str_mv Andersen, Natalia Denise
Veuthey, Tania Vanesa
Fariña, Milagros
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author Andersen, Natalia Denise
author_facet Andersen, Natalia Denise
Veuthey, Tania Vanesa
Fariña, Milagros
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author_role author
author2 Veuthey, Tania Vanesa
Fariña, Milagros
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv C ELEGANS
AGING
NEURODEGENERATION
IMIDAZOLIUM SALTS
topic C ELEGANS
AGING
NEURODEGENERATION
IMIDAZOLIUM SALTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, we aim to evaluate the role of imidazolium salts as antioxidant and antiaging agents. We synthesized imidazolium salts and use the nematode C. elegans to performed a screening to analyze their ability to improve oxidative stress (OS) resistance. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl)imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to evaluate MSI role in neurodegenerative models, and, ii) to describe the mechanism of action underlying its protective effects. There is a theory that links OS to aging and neurodegeneration. To evaluate MSI neuroprotection, we used C. elegans models of neurodegenerative diseases. As an Alzheimer disease (AD) model, we used a strain that expresses Aβ1-42 in muscle and shows age-dependent protein aggregation and paralysis. Our results show that MSI delays paralysis in this strain. Since mean lifespan is preserved in wild-type worms exposed to MSI, anti-aging effects of MSI in AD model seems to be dependent on its antiproteotoxic role. To gain further insight into its role in other neurotoxic models, we evaluate mobility as an indicator of healthspan in Huntington disease (HD) and Parkinson disease (PD) models. We found that MSI ameliorates mobility rate decline in these proteotoxic models of neurodegenerative diseases. As a first approach to delineate its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. We found that MSI stress protection was not dependent on DAF16. Therefore, other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. Additional research is needed to underpin the mechanism responsible for MSI’s protective role and to confirm if these effects can be extrapolated to other neurodegenerative scenarios.
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Fariña, Milagros. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
2nd Latin American Worm Meeting
Rosario
Argentina
Latin American Worm Meeting
description In this study, we aim to evaluate the role of imidazolium salts as antioxidant and antiaging agents. We synthesized imidazolium salts and use the nematode C. elegans to performed a screening to analyze their ability to improve oxidative stress (OS) resistance. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl)imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to evaluate MSI role in neurodegenerative models, and, ii) to describe the mechanism of action underlying its protective effects. There is a theory that links OS to aging and neurodegeneration. To evaluate MSI neuroprotection, we used C. elegans models of neurodegenerative diseases. As an Alzheimer disease (AD) model, we used a strain that expresses Aβ1-42 in muscle and shows age-dependent protein aggregation and paralysis. Our results show that MSI delays paralysis in this strain. Since mean lifespan is preserved in wild-type worms exposed to MSI, anti-aging effects of MSI in AD model seems to be dependent on its antiproteotoxic role. To gain further insight into its role in other neurotoxic models, we evaluate mobility as an indicator of healthspan in Huntington disease (HD) and Parkinson disease (PD) models. We found that MSI ameliorates mobility rate decline in these proteotoxic models of neurodegenerative diseases. As a first approach to delineate its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. We found that MSI stress protection was not dependent on DAF16. Therefore, other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. Additional research is needed to underpin the mechanism responsible for MSI’s protective role and to confirm if these effects can be extrapolated to other neurodegenerative scenarios.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217143
Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration; 2nd Latin American Worm Meeting; Rosario; Argentina; 2020; 40-40
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217143
identifier_str_mv Protective roles of imidazolium salts in C. elegans models of stress and neurodegeneration; 2nd Latin American Worm Meeting; Rosario; Argentina; 2020; 40-40
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://lawm2020.webnode.es/abstract-submission/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Rosario
publisher.none.fl_str_mv Universidad Nacional de Rosario
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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