New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration

Autores
Andersen, Natalia Denise; Veuthey, Tania Vanesa; Silbestri, Gustavo Fabián; Rayes, Diego Hernán; de Rosa, Maria Jose
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Imidazolium salts are attractive pharmacological agents that have been linked to a wide range of biological effects, including antitumoral, antimicrobial, anthelmintic and anti-inflammatory. In this study, we aim to evaluate the role of these compounds as antioxidant and anti- aging agents. We synthesized imidazolium salts and analyzed their ability to improve oxidative stress (OS) resistance. We used an established model in biomedical research, the free-living nematode C. elegans, and exposed them to the oxidizing agent FeSO4. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl) imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to describe MSI action mechanisms and, ii) to evaluate MSI role in neurodegenerative models. To gain insight into its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. Unexpectedly, our experiments revealed that MSI stress protection was not dependent on DAF-16. These results support the idea that other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. We are currently performing experiments to identify the role of these molecular players, in MSI-induced stress resistance. The second goal is held by the theory that links OS to aging and neurodegeneration. We are currently evaluating if MSI increases lifespan, healthspan, and improves biological markers of neurodegeneration in a C. elegans model of Alzheimer disease. This strain expresses Aß1-42 in muscle and shows age- dependent protein aggregation and paralysis. Our preliminary results show that MSI delays paralysis in this strain. Additional research is needed to underpin the protective role of MSI and to determine if these effects can be extrapolated in other neurodegenerative scenarios.
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
Materia
Neurodegeneration
Model
Proteotoxicity
Oxidative Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/188117
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/188117
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegenerationAndersen, Natalia DeniseVeuthey, Tania VanesaSilbestri, Gustavo FabiánRayes, Diego Hernánde Rosa, Maria JoseNeurodegenerationModelProteotoxicityOxidative Stresshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Imidazolium salts are attractive pharmacological agents that have been linked to a wide range of biological effects, including antitumoral, antimicrobial, anthelmintic and anti-inflammatory. In this study, we aim to evaluate the role of these compounds as antioxidant and anti- aging agents. We synthesized imidazolium salts and analyzed their ability to improve oxidative stress (OS) resistance. We used an established model in biomedical research, the free-living nematode C. elegans, and exposed them to the oxidizing agent FeSO4. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl) imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to describe MSI action mechanisms and, ii) to evaluate MSI role in neurodegenerative models. To gain insight into its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. Unexpectedly, our experiments revealed that MSI stress protection was not dependent on DAF-16. These results support the idea that other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. We are currently performing experiments to identify the role of these molecular players, in MSI-induced stress resistance. The second goal is held by the theory that links OS to aging and neurodegeneration. We are currently evaluating if MSI increases lifespan, healthspan, and improves biological markers of neurodegeneration in a C. elegans model of Alzheimer disease. This strain expresses Aß1-42 in muscle and shows age- dependent protein aggregation and paralysis. Our preliminary results show that MSI delays paralysis in this strain. Additional research is needed to underpin the protective role of MSI and to determine if these effects can be extrapolated in other neurodegenerative scenarios.Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioThe Histochemical SocietyFundación Revista MedicinaKotsias, Basilio Aristidesde Vito, EduardoNarvaiz Kantor, IsabelSemeniuk, Guillermo Basilio2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/188117New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 271-2710025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:33Zoai:ri.conicet.gov.ar:11336/188117instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:33.335CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
title New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
spellingShingle New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
Andersen, Natalia Denise
Neurodegeneration
Model
Proteotoxicity
Oxidative Stress
title_short New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
title_full New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
title_fullStr New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
title_full_unstemmed New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
title_sort New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration
dc.creator.none.fl_str_mv Andersen, Natalia Denise
Veuthey, Tania Vanesa
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author Andersen, Natalia Denise
author_facet Andersen, Natalia Denise
Veuthey, Tania Vanesa
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author_role author
author2 Veuthey, Tania Vanesa
Silbestri, Gustavo Fabián
Rayes, Diego Hernán
de Rosa, Maria Jose
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Kotsias, Basilio Aristides
de Vito, Eduardo
Narvaiz Kantor, Isabel
Semeniuk, Guillermo Basilio
dc.subject.none.fl_str_mv Neurodegeneration
Model
Proteotoxicity
Oxidative Stress
topic Neurodegeneration
Model
Proteotoxicity
Oxidative Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Imidazolium salts are attractive pharmacological agents that have been linked to a wide range of biological effects, including antitumoral, antimicrobial, anthelmintic and anti-inflammatory. In this study, we aim to evaluate the role of these compounds as antioxidant and anti- aging agents. We synthesized imidazolium salts and analyzed their ability to improve oxidative stress (OS) resistance. We used an established model in biomedical research, the free-living nematode C. elegans, and exposed them to the oxidizing agent FeSO4. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl) imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to describe MSI action mechanisms and, ii) to evaluate MSI role in neurodegenerative models. To gain insight into its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. Unexpectedly, our experiments revealed that MSI stress protection was not dependent on DAF-16. These results support the idea that other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. We are currently performing experiments to identify the role of these molecular players, in MSI-induced stress resistance. The second goal is held by the theory that links OS to aging and neurodegeneration. We are currently evaluating if MSI increases lifespan, healthspan, and improves biological markers of neurodegeneration in a C. elegans model of Alzheimer disease. This strain expresses Aß1-42 in muscle and shows age- dependent protein aggregation and paralysis. Our preliminary results show that MSI delays paralysis in this strain. Additional research is needed to underpin the protective role of MSI and to determine if these effects can be extrapolated in other neurodegenerative scenarios.
Fil: Andersen, Natalia Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Veuthey, Tania Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Silbestri, Gustavo Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
The Histochemical Society
description Imidazolium salts are attractive pharmacological agents that have been linked to a wide range of biological effects, including antitumoral, antimicrobial, anthelmintic and anti-inflammatory. In this study, we aim to evaluate the role of these compounds as antioxidant and anti- aging agents. We synthesized imidazolium salts and analyzed their ability to improve oxidative stress (OS) resistance. We used an established model in biomedical research, the free-living nematode C. elegans, and exposed them to the oxidizing agent FeSO4. We identified a derivate, 1-Mesithyl-3-(3-sulfonatopropyl) imidazolium (MSI), that enhances animal resistance to OS. To delineate MSI roles, we split this work into two goals: i) to describe MSI action mechanisms and, ii) to evaluate MSI role in neurodegenerative models. To gain insight into its mechanism of action, we evaluated MSI ability to activate DAF-16 (FOXO in vertebrates), a transcription factor relevant for cytoprotective defense mechanisms. Unexpectedly, our experiments revealed that MSI stress protection was not dependent on DAF-16. These results support the idea that other transcription factors (such as SKN-1 (Nrf-2 in vertebrates), HSF-1), could be involved in MSI protection. We are currently performing experiments to identify the role of these molecular players, in MSI-induced stress resistance. The second goal is held by the theory that links OS to aging and neurodegeneration. We are currently evaluating if MSI increases lifespan, healthspan, and improves biological markers of neurodegeneration in a C. elegans model of Alzheimer disease. This strain expresses Aß1-42 in muscle and shows age- dependent protein aggregation and paralysis. Our preliminary results show that MSI delays paralysis in this strain. Additional research is needed to underpin the protective role of MSI and to determine if these effects can be extrapolated in other neurodegenerative scenarios.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/188117
New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 271-271
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/188117
identifier_str_mv New findings for old players: Imidazolium salts as protective drugs in C. elegans models of stress and neurodegeneration; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimenta; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 271-271
0025-7680
1669-9106
CONICET Digital
CONICET
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publisher.none.fl_str_mv Fundación Revista Medicina
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