MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction

Autores
Cortés Campos, Christian; Elizondo, Roberto; Llanos, Paula; Uranga, Romina Maria; Nualart, Francisco; García, María de Los Ángeles
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.
Fil: Cortés Campos, Christian. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Elizondo, Roberto. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Llanos, Paula. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Nualart, Francisco. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: García, María de Los Ángeles. Universidad de Concepción. Departamento de Biología Celular; Chile
Materia
GLUCOSENSING
TANYCYTES
MONOCARBOXYLATE TRANSPORTERS
LACTATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7805
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7805
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interactionCortés Campos, ChristianElizondo, RobertoLlanos, PaulaUranga, Romina MariaNualart, FranciscoGarcía, María de Los ÁngelesGLUCOSENSINGTANYCYTESMONOCARBOXYLATE TRANSPORTERSLACTATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.Fil: Cortés Campos, Christian. Universidad de Concepción. Departamento de Biología Celular; ChileFil: Elizondo, Roberto. Universidad de Concepción. Departamento de Biología Celular; ChileFil: Llanos, Paula. Universidad de Concepción. Departamento de Biología Celular; ChileFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Nualart, Francisco. Universidad de Concepción. Departamento de Biología Celular; ChileFil: García, María de Los Ángeles. Universidad de Concepción. Departamento de Biología Celular; ChilePublic Library Of Science2011-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7805Cortés Campos, Christian; Elizondo, Roberto; Llanos, Paula; Uranga, Romina Maria; Nualart, Francisco; et al.; MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction; Public Library Of Science; Plos One; 6; 1; 28-1-2011; e164111932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0016411info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030577/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016411info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:43Zoai:ri.conicet.gov.ar:11336/7805instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:43.963CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
title MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
spellingShingle MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
Cortés Campos, Christian
GLUCOSENSING
TANYCYTES
MONOCARBOXYLATE TRANSPORTERS
LACTATE
title_short MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
title_full MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
title_fullStr MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
title_full_unstemmed MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
title_sort MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction
dc.creator.none.fl_str_mv Cortés Campos, Christian
Elizondo, Roberto
Llanos, Paula
Uranga, Romina Maria
Nualart, Francisco
García, María de Los Ángeles
author Cortés Campos, Christian
author_facet Cortés Campos, Christian
Elizondo, Roberto
Llanos, Paula
Uranga, Romina Maria
Nualart, Francisco
García, María de Los Ángeles
author_role author
author2 Elizondo, Roberto
Llanos, Paula
Uranga, Romina Maria
Nualart, Francisco
García, María de Los Ángeles
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GLUCOSENSING
TANYCYTES
MONOCARBOXYLATE TRANSPORTERS
LACTATE
topic GLUCOSENSING
TANYCYTES
MONOCARBOXYLATE TRANSPORTERS
LACTATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.
Fil: Cortés Campos, Christian. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Elizondo, Roberto. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Llanos, Paula. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Nualart, Francisco. Universidad de Concepción. Departamento de Biología Celular; Chile
Fil: García, María de Los Ángeles. Universidad de Concepción. Departamento de Biología Celular; Chile
description Metabolic interaction via lactate between glial cells and neurons has been proposed as one of the mechanisms involved in hypothalamic glucosensing. We have postulated that hypothalamic glial cells, also known as tanycytes, produce lactate by glycolytic metabolism of glucose. Transfer of lactate to neighboring neurons stimulates ATP synthesis and thus contributes to their activation. Because destruction of third ventricle (III-V) tanycytes is sufficient to alter blood glucose levels and food intake in rats, it is hypothesized that tanycytes are involved in the hypothalamic glucose sensing mechanism. Here, we demonstrate the presence and function of monocarboxylate transporters (MCTs) in tanycytes. Specifically, MCT1 and MCT4 expression as well as their distribution were analyzed in Sprague Dawley rat brain, and we demonstrate that both transporters are expressed in tanycytes. Using primary tanycyte cultures, kinetic analyses and sensitivity to inhibitors were undertaken to confirm that MCT1 and MCT4 were functional for lactate influx. Additionally, physiological concentrations of glucose induced lactate efflux in cultured tanycytes, which was inhibited by classical MCT inhibitors. Because the expression of both MCT1 and MCT4 has been linked to lactate efflux, we propose that tanycytes participate in glucose sensing based on a metabolic interaction with neurons of the arcuate nucleus, which are stimulated by lactate released from MCT1 and MCT4-expressing tanycytes.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7805
Cortés Campos, Christian; Elizondo, Roberto; Llanos, Paula; Uranga, Romina Maria; Nualart, Francisco; et al.; MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction; Public Library Of Science; Plos One; 6; 1; 28-1-2011; e16411
1932-6203
url http://hdl.handle.net/11336/7805
identifier_str_mv Cortés Campos, Christian; Elizondo, Roberto; Llanos, Paula; Uranga, Romina Maria; Nualart, Francisco; et al.; MCT expression and lactate influx/efflux in tanycytes involved in glia-neuron metabolic interaction; Public Library Of Science; Plos One; 6; 1; 28-1-2011; e16411
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0016411
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3030577/
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0016411
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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