Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
- Autores
- Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; Offermanns, Stefan; Sirard, Jean Claude; Rumbo, Martín
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; Francia
Fil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina - Materia
-
LACTATE
MACROPHAGE
METABOLIC REPROGRAMMING
INFLAMMATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48537
Ver los metadatos del registro completo
id |
CONICETDig_140b47bf17c8bcdcf16370543ef1493b |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/48537 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent MannerErrea, Agustina JulianaCayet, DelphineMarchetti, PhilippeTang, CongKluza, JeromeOffermanns, StefanSirard, Jean ClaudeRumbo, MartínLACTATEMACROPHAGEMETABOLIC REPROGRAMMINGINFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; FranciaFil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; FranciaFil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; FranciaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaPublic Library of Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48537Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e01636941932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0163694info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:30Zoai:ri.conicet.gov.ar:11336/48537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:30.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
title |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
spellingShingle |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner Errea, Agustina Juliana LACTATE MACROPHAGE METABOLIC REPROGRAMMING INFLAMMATION |
title_short |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
title_full |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
title_fullStr |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
title_full_unstemmed |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
title_sort |
Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner |
dc.creator.none.fl_str_mv |
Errea, Agustina Juliana Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean Claude Rumbo, Martín |
author |
Errea, Agustina Juliana |
author_facet |
Errea, Agustina Juliana Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean Claude Rumbo, Martín |
author_role |
author |
author2 |
Cayet, Delphine Marchetti, Philippe Tang, Cong Kluza, Jerome Offermanns, Stefan Sirard, Jean Claude Rumbo, Martín |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
LACTATE MACROPHAGE METABOLIC REPROGRAMMING INFLAMMATION |
topic |
LACTATE MACROPHAGE METABOLIC REPROGRAMMING INFLAMMATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation. Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina Fil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia Fil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; Francia Fil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; Alemania Fil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; Alemania Fil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; Alemania Fil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina |
description |
Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48537 Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e0163694 1932-6203 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48537 |
identifier_str_mv |
Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e0163694 1932-6203 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0163694 info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163694 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614032859856896 |
score |
13.070432 |