Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner

Autores
Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; Offermanns, Stefan; Sirard, Jean Claude; Rumbo, Martín
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; Francia
Fil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Materia
LACTATE
MACROPHAGE
METABOLIC REPROGRAMMING
INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48537
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent MannerErrea, Agustina JulianaCayet, DelphineMarchetti, PhilippeTang, CongKluza, JeromeOffermanns, StefanSirard, Jean ClaudeRumbo, MartínLACTATEMACROPHAGEMETABOLIC REPROGRAMMINGINFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; FranciaFil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; FranciaFil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; AlemaniaFil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; FranciaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaPublic Library of Science2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48537Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e01636941932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0163694info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:57:13Zoai:ri.conicet.gov.ar:11336/48537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:57:13.359CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
title Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
spellingShingle Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
Errea, Agustina Juliana
LACTATE
MACROPHAGE
METABOLIC REPROGRAMMING
INFLAMMATION
title_short Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
title_full Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
title_fullStr Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
title_full_unstemmed Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
title_sort Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner
dc.creator.none.fl_str_mv Errea, Agustina Juliana
Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean Claude
Rumbo, Martín
author Errea, Agustina Juliana
author_facet Errea, Agustina Juliana
Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean Claude
Rumbo, Martín
author_role author
author2 Cayet, Delphine
Marchetti, Philippe
Tang, Cong
Kluza, Jerome
Offermanns, Stefan
Sirard, Jean Claude
Rumbo, Martín
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTATE
MACROPHAGE
METABOLIC REPROGRAMMING
INFLAMMATION
topic LACTATE
MACROPHAGE
METABOLIC REPROGRAMMING
INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
Fil: Errea, Agustina Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Cayet, Delphine. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Marchetti, Philippe. Universite Lille 3; Francia. Inserm; Francia. Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer; Francia
Fil: Tang, Cong. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Kluza, Jerome. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Offermanns, Stefan. Max Planck Institute for Heart and Lung Research study; Alemania
Fil: Sirard, Jean Claude. Centre National de la Recherche Scientifique; Francia. Universite Lille 3; Francia. Instituto Pasteur; Francia
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
description Lactate is an essential component of carbon metabolism in mammals. Recently, lactate was shown to signal through the G protein coupled receptor 81 (GPR81) and to thus modulate inflammatory processes. This study demonstrates that lactate inhibits pro-inflammatory signaling in a GPR81-independent fashion. While lipopolysaccharide (LPS) triggered expression of IL-6 and IL-12 p40, and CD40 in bone marrow-derived macrophages, lactate was able to abrogate these responses in a dose dependent manner in Gpr81-/- cells as well as in wild type cells. Macrophage activation was impaired when glycolysis was blocked by chemical inhibitors. Remarkably, lactate was found to inhibit LPS-induced glycolysis in wild type as well as in Gpr81-/- cells. In conclusion, our study suggests that lactate can induce GPR81-independent metabolic changes that modulate macrophage pro-inflammatory activation.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48537
Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e0163694
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48537
identifier_str_mv Errea, Agustina Juliana; Cayet, Delphine; Marchetti, Philippe; Tang, Cong; Kluza, Jerome; et al.; Lactate Inhibits the Pro-Inflammatory Response and Metabolic Reprogramming in Murine Macrophages in a GPR81-Independent Manner; Public Library of Science; Plos One; 11; 11; 11-2016; 1-11; e0163694
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0163694
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0163694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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