Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior

Autores
Uranga, Romina Maria; Millán, Carola; Barahona, María José; Recabal, Antonia; Salgado, Magdiel; Martinez, Fernando; Ordenes, Patricio; Elizondo Vega, Roberto; Sepúlveda, Fernando; Uribe, Elena; García Robles, María de los Ángeles
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Glucokinase (GK), the hexokinase involved in glucosensing in pancreatic β-cells, is also expressed in arcuate nucleus (AN) neurons and hypothalamic tanycytes, the cells that surround the basal third ventricle (3V). Several lines of evidence suggest that tanycytes may be involved in the regulation of energy homeostasis. Tanycytes have extended cell processes that contact the feeding-regulating neurons in the AN, particularly, agouti-related protein (AgRP), neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC) neurons. In this study, we developed an adenovirus expressing GK shRNA to inhibit GK expression in vivo. When injected into the 3V of rats, this adenovirus preferentially transduced tanycytes. qRT-PCR and Western blot assays confirmed GK mRNA and protein levels were lower in GK knockdown animals compared to the controls. In response to an intracerebroventricular glucose injection, the mRNA levels of anorexigenic POMC and CART and orexigenic AgRP and NPY neuropeptides were altered in GK knockdown animals. Similarly, food intake, meal duration, frequency of eating events and the cumulative eating time were increased, whereas the intervals between meals were decreased in GK knockdown rats, suggesting a decrease in satiety. Thus, GK expression in the ventricular cells appears to play an important role in feeding behavior.
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad de Concepción; Chile
Fil: Millán, Carola. Universidad de Concepción; Chile. Universidad Adolfo Ibañez; Chile
Fil: Barahona, María José. Universidad de Concepción; Chile
Fil: Recabal, Antonia. Universidad de Concepción; Chile
Fil: Salgado, Magdiel. Universidad de Concepción; Chile
Fil: Martinez, Fernando. Universidad de Concepción; Chile
Fil: Ordenes, Patricio. Universidad de Concepción; Chile
Fil: Elizondo Vega, Roberto. Universidad de Concepción; Chile
Fil: Sepúlveda, Fernando. Universidad de Concepción; Chile
Fil: Uribe, Elena. Universidad de Concepción; Chile
Fil: García Robles, María de los Ángeles. Universidad de Concepción; Chile
Materia
GLUCOKINASE
TANYCYTES
HIPOTHALAMUS
FEEDING BEHAVIOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41025
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41025
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behaviorUranga, Romina MariaMillán, CarolaBarahona, María JoséRecabal, AntoniaSalgado, MagdielMartinez, FernandoOrdenes, PatricioElizondo Vega, RobertoSepúlveda, FernandoUribe, ElenaGarcía Robles, María de los ÁngelesGLUCOKINASETANYCYTESHIPOTHALAMUSFEEDING BEHAVIORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Glucokinase (GK), the hexokinase involved in glucosensing in pancreatic β-cells, is also expressed in arcuate nucleus (AN) neurons and hypothalamic tanycytes, the cells that surround the basal third ventricle (3V). Several lines of evidence suggest that tanycytes may be involved in the regulation of energy homeostasis. Tanycytes have extended cell processes that contact the feeding-regulating neurons in the AN, particularly, agouti-related protein (AgRP), neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC) neurons. In this study, we developed an adenovirus expressing GK shRNA to inhibit GK expression in vivo. When injected into the 3V of rats, this adenovirus preferentially transduced tanycytes. qRT-PCR and Western blot assays confirmed GK mRNA and protein levels were lower in GK knockdown animals compared to the controls. In response to an intracerebroventricular glucose injection, the mRNA levels of anorexigenic POMC and CART and orexigenic AgRP and NPY neuropeptides were altered in GK knockdown animals. Similarly, food intake, meal duration, frequency of eating events and the cumulative eating time were increased, whereas the intervals between meals were decreased in GK knockdown rats, suggesting a decrease in satiety. Thus, GK expression in the ventricular cells appears to play an important role in feeding behavior.Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad de Concepción; ChileFil: Millán, Carola. Universidad de Concepción; Chile. Universidad Adolfo Ibañez; ChileFil: Barahona, María José. Universidad de Concepción; ChileFil: Recabal, Antonia. Universidad de Concepción; ChileFil: Salgado, Magdiel. Universidad de Concepción; ChileFil: Martinez, Fernando. Universidad de Concepción; ChileFil: Ordenes, Patricio. Universidad de Concepción; ChileFil: Elizondo Vega, Roberto. Universidad de Concepción; ChileFil: Sepúlveda, Fernando. Universidad de Concepción; ChileFil: Uribe, Elena. Universidad de Concepción; ChileFil: García Robles, María de los Ángeles. Universidad de Concepción; ChileNature Publishing Group2017-06-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41025Uranga, Romina Maria; Millán, Carola; Barahona, María José; Recabal, Antonia; Salgado, Magdiel; et al.; Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior; Nature Publishing Group; Scientific Reports; 7; 1; 16-6-2017; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-03928-xinfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-03928-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:45Zoai:ri.conicet.gov.ar:11336/41025instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:46.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
title Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
spellingShingle Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
Uranga, Romina Maria
GLUCOKINASE
TANYCYTES
HIPOTHALAMUS
FEEDING BEHAVIOR
title_short Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
title_full Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
title_fullStr Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
title_full_unstemmed Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
title_sort Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior
dc.creator.none.fl_str_mv Uranga, Romina Maria
Millán, Carola
Barahona, María José
Recabal, Antonia
Salgado, Magdiel
Martinez, Fernando
Ordenes, Patricio
Elizondo Vega, Roberto
Sepúlveda, Fernando
Uribe, Elena
García Robles, María de los Ángeles
author Uranga, Romina Maria
author_facet Uranga, Romina Maria
Millán, Carola
Barahona, María José
Recabal, Antonia
Salgado, Magdiel
Martinez, Fernando
Ordenes, Patricio
Elizondo Vega, Roberto
Sepúlveda, Fernando
Uribe, Elena
García Robles, María de los Ángeles
author_role author
author2 Millán, Carola
Barahona, María José
Recabal, Antonia
Salgado, Magdiel
Martinez, Fernando
Ordenes, Patricio
Elizondo Vega, Roberto
Sepúlveda, Fernando
Uribe, Elena
García Robles, María de los Ángeles
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GLUCOKINASE
TANYCYTES
HIPOTHALAMUS
FEEDING BEHAVIOR
topic GLUCOKINASE
TANYCYTES
HIPOTHALAMUS
FEEDING BEHAVIOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Glucokinase (GK), the hexokinase involved in glucosensing in pancreatic β-cells, is also expressed in arcuate nucleus (AN) neurons and hypothalamic tanycytes, the cells that surround the basal third ventricle (3V). Several lines of evidence suggest that tanycytes may be involved in the regulation of energy homeostasis. Tanycytes have extended cell processes that contact the feeding-regulating neurons in the AN, particularly, agouti-related protein (AgRP), neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC) neurons. In this study, we developed an adenovirus expressing GK shRNA to inhibit GK expression in vivo. When injected into the 3V of rats, this adenovirus preferentially transduced tanycytes. qRT-PCR and Western blot assays confirmed GK mRNA and protein levels were lower in GK knockdown animals compared to the controls. In response to an intracerebroventricular glucose injection, the mRNA levels of anorexigenic POMC and CART and orexigenic AgRP and NPY neuropeptides were altered in GK knockdown animals. Similarly, food intake, meal duration, frequency of eating events and the cumulative eating time were increased, whereas the intervals between meals were decreased in GK knockdown rats, suggesting a decrease in satiety. Thus, GK expression in the ventricular cells appears to play an important role in feeding behavior.
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad de Concepción; Chile
Fil: Millán, Carola. Universidad de Concepción; Chile. Universidad Adolfo Ibañez; Chile
Fil: Barahona, María José. Universidad de Concepción; Chile
Fil: Recabal, Antonia. Universidad de Concepción; Chile
Fil: Salgado, Magdiel. Universidad de Concepción; Chile
Fil: Martinez, Fernando. Universidad de Concepción; Chile
Fil: Ordenes, Patricio. Universidad de Concepción; Chile
Fil: Elizondo Vega, Roberto. Universidad de Concepción; Chile
Fil: Sepúlveda, Fernando. Universidad de Concepción; Chile
Fil: Uribe, Elena. Universidad de Concepción; Chile
Fil: García Robles, María de los Ángeles. Universidad de Concepción; Chile
description Glucokinase (GK), the hexokinase involved in glucosensing in pancreatic β-cells, is also expressed in arcuate nucleus (AN) neurons and hypothalamic tanycytes, the cells that surround the basal third ventricle (3V). Several lines of evidence suggest that tanycytes may be involved in the regulation of energy homeostasis. Tanycytes have extended cell processes that contact the feeding-regulating neurons in the AN, particularly, agouti-related protein (AgRP), neuropeptide Y (NPY), cocaine- and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC) neurons. In this study, we developed an adenovirus expressing GK shRNA to inhibit GK expression in vivo. When injected into the 3V of rats, this adenovirus preferentially transduced tanycytes. qRT-PCR and Western blot assays confirmed GK mRNA and protein levels were lower in GK knockdown animals compared to the controls. In response to an intracerebroventricular glucose injection, the mRNA levels of anorexigenic POMC and CART and orexigenic AgRP and NPY neuropeptides were altered in GK knockdown animals. Similarly, food intake, meal duration, frequency of eating events and the cumulative eating time were increased, whereas the intervals between meals were decreased in GK knockdown rats, suggesting a decrease in satiety. Thus, GK expression in the ventricular cells appears to play an important role in feeding behavior.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41025
Uranga, Romina Maria; Millán, Carola; Barahona, María José; Recabal, Antonia; Salgado, Magdiel; et al.; Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior; Nature Publishing Group; Scientific Reports; 7; 1; 16-6-2017; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41025
identifier_str_mv Uranga, Romina Maria; Millán, Carola; Barahona, María José; Recabal, Antonia; Salgado, Magdiel; et al.; Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior; Nature Publishing Group; Scientific Reports; 7; 1; 16-6-2017; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-03928-x
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-03928-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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