N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films

Autores
Dupuy, Fernando Gabriel; Maggio, Bruno
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sphingolipids are membrane lipids composed by a long chain aminediol base, usually sphingosine, with a N-linked fatty acyl chain whose quality depends on the membrane type. The effect of length and unsaturation of the N-acyl chain on the mixing behavior of different sphingolipids has scarcely been studied, and in this work this issue is addressed employing Langmuir monolayers at the air–water interface, in order to assess the surface mixing in binary mixtures of different species of sphingomyelins and ceramides. The dependence on the monolayer composition of the mean molecular area, perpendicular dipole moment, domain segregation, and surface topography, as well as the film elasticity and optical thickness were studied. The results indicate that composition-dependent favorable interactions among sphingomyelin and ceramide occur as a consequence of complementary lateral packing and increased acyl chain ordering; the phase state of the components appears as a major factor determining miscibility among sphingomyelins and ceramides even in cases where the lipids have a considerable hydrocarbon chain length mismatch.
Fil: Dupuy, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Materia
Sphingomyelin
Ceramide
Monolayer
Mixture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30218
Acceder
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network_name_str CONICET Digital (CONICET)
spelling N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir FilmsDupuy, Fernando GabrielMaggio, BrunoSphingomyelinCeramideMonolayerMixturehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sphingolipids are membrane lipids composed by a long chain aminediol base, usually sphingosine, with a N-linked fatty acyl chain whose quality depends on the membrane type. The effect of length and unsaturation of the N-acyl chain on the mixing behavior of different sphingolipids has scarcely been studied, and in this work this issue is addressed employing Langmuir monolayers at the air–water interface, in order to assess the surface mixing in binary mixtures of different species of sphingomyelins and ceramides. The dependence on the monolayer composition of the mean molecular area, perpendicular dipole moment, domain segregation, and surface topography, as well as the film elasticity and optical thickness were studied. The results indicate that composition-dependent favorable interactions among sphingomyelin and ceramide occur as a consequence of complementary lateral packing and increased acyl chain ordering; the phase state of the components appears as a major factor determining miscibility among sphingomyelins and ceramides even in cases where the lipids have a considerable hydrocarbon chain length mismatch.Fil: Dupuy, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaAmerican Chemical Society2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30218Dupuy, Fernando Gabriel; Maggio, Bruno; N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films; American Chemical Society; Journal of Physical Chemistry B; 118; 27; 6-2014; 7475-74871520-6106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1021/jp501686qinfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/jp501686qinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:20Zoai:ri.conicet.gov.ar:11336/30218instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:21.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
title N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
spellingShingle N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
Dupuy, Fernando Gabriel
Sphingomyelin
Ceramide
Monolayer
Mixture
title_short N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
title_full N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
title_fullStr N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
title_full_unstemmed N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
title_sort N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films
dc.creator.none.fl_str_mv Dupuy, Fernando Gabriel
Maggio, Bruno
author Dupuy, Fernando Gabriel
author_facet Dupuy, Fernando Gabriel
Maggio, Bruno
author_role author
author2 Maggio, Bruno
author2_role author
dc.subject.none.fl_str_mv Sphingomyelin
Ceramide
Monolayer
Mixture
topic Sphingomyelin
Ceramide
Monolayer
Mixture
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sphingolipids are membrane lipids composed by a long chain aminediol base, usually sphingosine, with a N-linked fatty acyl chain whose quality depends on the membrane type. The effect of length and unsaturation of the N-acyl chain on the mixing behavior of different sphingolipids has scarcely been studied, and in this work this issue is addressed employing Langmuir monolayers at the air–water interface, in order to assess the surface mixing in binary mixtures of different species of sphingomyelins and ceramides. The dependence on the monolayer composition of the mean molecular area, perpendicular dipole moment, domain segregation, and surface topography, as well as the film elasticity and optical thickness were studied. The results indicate that composition-dependent favorable interactions among sphingomyelin and ceramide occur as a consequence of complementary lateral packing and increased acyl chain ordering; the phase state of the components appears as a major factor determining miscibility among sphingomyelins and ceramides even in cases where the lipids have a considerable hydrocarbon chain length mismatch.
Fil: Dupuy, Fernando Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
description Sphingolipids are membrane lipids composed by a long chain aminediol base, usually sphingosine, with a N-linked fatty acyl chain whose quality depends on the membrane type. The effect of length and unsaturation of the N-acyl chain on the mixing behavior of different sphingolipids has scarcely been studied, and in this work this issue is addressed employing Langmuir monolayers at the air–water interface, in order to assess the surface mixing in binary mixtures of different species of sphingomyelins and ceramides. The dependence on the monolayer composition of the mean molecular area, perpendicular dipole moment, domain segregation, and surface topography, as well as the film elasticity and optical thickness were studied. The results indicate that composition-dependent favorable interactions among sphingomyelin and ceramide occur as a consequence of complementary lateral packing and increased acyl chain ordering; the phase state of the components appears as a major factor determining miscibility among sphingomyelins and ceramides even in cases where the lipids have a considerable hydrocarbon chain length mismatch.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30218
Dupuy, Fernando Gabriel; Maggio, Bruno; N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films; American Chemical Society; Journal of Physical Chemistry B; 118; 27; 6-2014; 7475-7487
1520-6106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30218
identifier_str_mv Dupuy, Fernando Gabriel; Maggio, Bruno; N-Acyl Chain in Ceramide and Sphingomyelin Determines Their Mixing Behavior, Phase State, and Surface Topography in Langmuir Films; American Chemical Society; Journal of Physical Chemistry B; 118; 27; 6-2014; 7475-7487
1520-6106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1021/jp501686q
info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/10.1021/jp501686q
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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