Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa

Autores
Furland, Natalia Edith; Oresti, Gerardo Martin; Antollini, Silvia Susana; Venturino, Andres; Maldonado, Eduardo N.; Aveldaño, Marta Isabel
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyunsaturated fatty acids (VLCPUFA) are important constituents of sphingomyelin (SM) and ceramide (Cer) in testicular germ cells. In the present paper we focused on the SM and Cer and their fatty acids inspermatozoa and their main regions, heads and tails. In bull and ram spermatozoa,SM was the third most abundant phospholipid and VLCPUFA were the major acylgroups ( approximately 70%) of SM and Cer. In rat epididymal spermatozoa the SM/Cer ratio was low in the absence of and could be maintained high in the presence of the cation chelator EDTA, added to the medium used for spermisolation. This fact points to the occurrence of an active divalent cation-dependent sphingomyelinase. Bull and rat sperm had an uneven head-tail distribution of phospholipid, with virtually all the VLCPUFA-rich SM located atthe head, the lower SM content in the rat being determined by the lower spermhead/tail size ratio. Most of the SM from bull sperm heads was readilysolubilized with 1% Triton X-100 at 4 degrees C. The detergent-soluble SMfraction was richer in VLCPUFA than the nonsoluble fraction and richer insaturated fatty acids. Cer was produced at the expense of SM, thus decreasing severalfold the SM/Cer ratio in rat spermatozoa incubated for 2 h in presence ofthe sperm-capacitating agents, calcium, bicarbonate, and albumin. The generationof Cer from SM in the sperm head surface may be an early step among thebiochemical and biophysical changes known to take place in the spermatozoon inthe physiological events preceding fertilization.
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maldonado, Eduardo N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Materia
CERAMIDES
SPHINGOMYELIN
SPERMATOZOA
VERY LONG CHAIN PUFA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148432
Acceder
id CONICETDig_d247256f97fa79b1d212feefe68959d4
oai_identifier_str oai:ri.conicet.gov.ar:11336/148432
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoaFurland, Natalia EdithOresti, Gerardo MartinAntollini, Silvia SusanaVenturino, AndresMaldonado, Eduardo N.Aveldaño, Marta IsabelCERAMIDESSPHINGOMYELINSPERMATOZOAVERY LONG CHAIN PUFAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Polyunsaturated fatty acids (VLCPUFA) are important constituents of sphingomyelin (SM) and ceramide (Cer) in testicular germ cells. In the present paper we focused on the SM and Cer and their fatty acids inspermatozoa and their main regions, heads and tails. In bull and ram spermatozoa,SM was the third most abundant phospholipid and VLCPUFA were the major acylgroups ( approximately 70%) of SM and Cer. In rat epididymal spermatozoa the SM/Cer ratio was low in the absence of and could be maintained high in the presence of the cation chelator EDTA, added to the medium used for spermisolation. This fact points to the occurrence of an active divalent cation-dependent sphingomyelinase. Bull and rat sperm had an uneven head-tail distribution of phospholipid, with virtually all the VLCPUFA-rich SM located atthe head, the lower SM content in the rat being determined by the lower spermhead/tail size ratio. Most of the SM from bull sperm heads was readilysolubilized with 1% Triton X-100 at 4 degrees C. The detergent-soluble SMfraction was richer in VLCPUFA than the nonsoluble fraction and richer insaturated fatty acids. Cer was produced at the expense of SM, thus decreasing severalfold the SM/Cer ratio in rat spermatozoa incubated for 2 h in presence ofthe sperm-capacitating agents, calcium, bicarbonate, and albumin. The generationof Cer from SM in the sperm head surface may be an early step among thebiochemical and biophysical changes known to take place in the spermatozoon inthe physiological events preceding fertilization.Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Venturino, Andres. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maldonado, Eduardo N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAmerican Society for Biochemistry and Molecular Biology2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148432Furland, Natalia Edith; Oresti, Gerardo Martin; Antollini, Silvia Susana; Venturino, Andres; Maldonado, Eduardo N.; et al.; Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 282; 25; 6-2007; 18151-181610021-9258CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jbc.org/article/S0021-9258(20)68917-7/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M700709200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:21Zoai:ri.conicet.gov.ar:11336/148432instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:21.971CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
title Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
spellingShingle Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
Furland, Natalia Edith
CERAMIDES
SPHINGOMYELIN
SPERMATOZOA
VERY LONG CHAIN PUFA
title_short Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
title_full Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
title_fullStr Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
title_full_unstemmed Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
title_sort Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa
dc.creator.none.fl_str_mv Furland, Natalia Edith
Oresti, Gerardo Martin
Antollini, Silvia Susana
Venturino, Andres
Maldonado, Eduardo N.
Aveldaño, Marta Isabel
author Furland, Natalia Edith
author_facet Furland, Natalia Edith
Oresti, Gerardo Martin
Antollini, Silvia Susana
Venturino, Andres
Maldonado, Eduardo N.
Aveldaño, Marta Isabel
author_role author
author2 Oresti, Gerardo Martin
Antollini, Silvia Susana
Venturino, Andres
Maldonado, Eduardo N.
Aveldaño, Marta Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CERAMIDES
SPHINGOMYELIN
SPERMATOZOA
VERY LONG CHAIN PUFA
topic CERAMIDES
SPHINGOMYELIN
SPERMATOZOA
VERY LONG CHAIN PUFA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyunsaturated fatty acids (VLCPUFA) are important constituents of sphingomyelin (SM) and ceramide (Cer) in testicular germ cells. In the present paper we focused on the SM and Cer and their fatty acids inspermatozoa and their main regions, heads and tails. In bull and ram spermatozoa,SM was the third most abundant phospholipid and VLCPUFA were the major acylgroups ( approximately 70%) of SM and Cer. In rat epididymal spermatozoa the SM/Cer ratio was low in the absence of and could be maintained high in the presence of the cation chelator EDTA, added to the medium used for spermisolation. This fact points to the occurrence of an active divalent cation-dependent sphingomyelinase. Bull and rat sperm had an uneven head-tail distribution of phospholipid, with virtually all the VLCPUFA-rich SM located atthe head, the lower SM content in the rat being determined by the lower spermhead/tail size ratio. Most of the SM from bull sperm heads was readilysolubilized with 1% Triton X-100 at 4 degrees C. The detergent-soluble SMfraction was richer in VLCPUFA than the nonsoluble fraction and richer insaturated fatty acids. Cer was produced at the expense of SM, thus decreasing severalfold the SM/Cer ratio in rat spermatozoa incubated for 2 h in presence ofthe sperm-capacitating agents, calcium, bicarbonate, and albumin. The generationof Cer from SM in the sperm head surface may be an early step among thebiochemical and biophysical changes known to take place in the spermatozoon inthe physiological events preceding fertilization.
Fil: Furland, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Venturino, Andres. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maldonado, Eduardo N.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
description Polyunsaturated fatty acids (VLCPUFA) are important constituents of sphingomyelin (SM) and ceramide (Cer) in testicular germ cells. In the present paper we focused on the SM and Cer and their fatty acids inspermatozoa and their main regions, heads and tails. In bull and ram spermatozoa,SM was the third most abundant phospholipid and VLCPUFA were the major acylgroups ( approximately 70%) of SM and Cer. In rat epididymal spermatozoa the SM/Cer ratio was low in the absence of and could be maintained high in the presence of the cation chelator EDTA, added to the medium used for spermisolation. This fact points to the occurrence of an active divalent cation-dependent sphingomyelinase. Bull and rat sperm had an uneven head-tail distribution of phospholipid, with virtually all the VLCPUFA-rich SM located atthe head, the lower SM content in the rat being determined by the lower spermhead/tail size ratio. Most of the SM from bull sperm heads was readilysolubilized with 1% Triton X-100 at 4 degrees C. The detergent-soluble SMfraction was richer in VLCPUFA than the nonsoluble fraction and richer insaturated fatty acids. Cer was produced at the expense of SM, thus decreasing severalfold the SM/Cer ratio in rat spermatozoa incubated for 2 h in presence ofthe sperm-capacitating agents, calcium, bicarbonate, and albumin. The generationof Cer from SM in the sperm head surface may be an early step among thebiochemical and biophysical changes known to take place in the spermatozoon inthe physiological events preceding fertilization.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148432
Furland, Natalia Edith; Oresti, Gerardo Martin; Antollini, Silvia Susana; Venturino, Andres; Maldonado, Eduardo N.; et al.; Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 282; 25; 6-2007; 18151-18161
0021-9258
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148432
identifier_str_mv Furland, Natalia Edith; Oresti, Gerardo Martin; Antollini, Silvia Susana; Venturino, Andres; Maldonado, Eduardo N.; et al.; Very long chain polyunsaturated fatty acids are the major acyl groups of sphingomyelins and ceramides located in the head of bull and rat spermatozoa; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry (online); 282; 25; 6-2007; 18151-18161
0021-9258
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jbc.org/article/S0021-9258(20)68917-7/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1074/jbc.M700709200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613651596574720
score 13.070432

Publicaciones similares