Surface behavior of ceramides with very long chain polyunsaturated fatty acids

Autores
Peñalva, Daniel Alejandro; Fanani, Maria Laura; Oresti, Gerardo Martin; Antollini, Silvia Susana; Maggio, Bruno; Aveldaño, Marta Isabel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Molecular species of sphingomyelin (SM) and ceramide (Cer) with nonhydroxy and 2-hydroxy very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) abound in mammalian spermatogenic cells and spermatozoa. In rat sperm, Cer naturally occurs in the tail and is produced from SM in the head once the acrosomal reaction is completed. The properties these atypical lipids confer to membranes remain to be defined. The aim of this study was to evaluate surface properties of isolated molecular species of Cer in monolayers. Ceramides containing nonhydroxy and 2-hydroxy fatty acids like 28:4, 30:5 and 32:5 isolated from rat testes, were resolved by a combination of chromatographic techniques including HPLC. Langmuir monolayer studies allowed to measure surface pressure, surface potential and reflectivity images by Brewster angle microscopy of these Cer spread at the air-water interface. We were able to determine average molecular area (degree of packing), surface potential, and thickness of these films. Isotherms of these molecular species showed expanded phases at large molecular areas and in-plane phase transitions to a liquid condensed state at lower molecular areas at both 7°C and 21°C. In the transition region, the formation of domains with different shapes could be appreciated. Our results show that ceramide surface properties depend on fatty acid unsaturation, chain length and the presence of the 2-hydroxyl group in the acyl chain. Furthermore, most of these properties differed significantly from those of previously characterized Cer with long-chain saturated fatty acids.
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLI Reunión Anual de la Sociedad Argentina de Biofísica
Tucumán
Argentina
Sociedad Argentina de Biofísica
Materia
CERAMIDE
SPHINGOLIPIDS
LANGMUIR MONOLAYER
VLCPUFA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/252701
Acceder
id CONICETDig_ba84e1585a70bab726099983b6695be6
oai_identifier_str oai:ri.conicet.gov.ar:11336/252701
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Surface behavior of ceramides with very long chain polyunsaturated fatty acidsPeñalva, Daniel AlejandroFanani, Maria LauraOresti, Gerardo MartinAntollini, Silvia SusanaMaggio, BrunoAveldaño, Marta IsabelCERAMIDESPHINGOLIPIDSLANGMUIR MONOLAYERVLCPUFAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Molecular species of sphingomyelin (SM) and ceramide (Cer) with nonhydroxy and 2-hydroxy very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) abound in mammalian spermatogenic cells and spermatozoa. In rat sperm, Cer naturally occurs in the tail and is produced from SM in the head once the acrosomal reaction is completed. The properties these atypical lipids confer to membranes remain to be defined. The aim of this study was to evaluate surface properties of isolated molecular species of Cer in monolayers. Ceramides containing nonhydroxy and 2-hydroxy fatty acids like 28:4, 30:5 and 32:5 isolated from rat testes, were resolved by a combination of chromatographic techniques including HPLC. Langmuir monolayer studies allowed to measure surface pressure, surface potential and reflectivity images by Brewster angle microscopy of these Cer spread at the air-water interface. We were able to determine average molecular area (degree of packing), surface potential, and thickness of these films. Isotherms of these molecular species showed expanded phases at large molecular areas and in-plane phase transitions to a liquid condensed state at lower molecular areas at both 7°C and 21°C. In the transition region, the formation of domains with different shapes could be appreciated. Our results show that ceramide surface properties depend on fatty acid unsaturation, chain length and the presence of the 2-hydroxyl group in the acyl chain. Furthermore, most of these properties differed significantly from those of previously characterized Cer with long-chain saturated fatty acids.Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLI Reunión Anual de la Sociedad Argentina de BiofísicaTucumánArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaChehin, Rosana NievesAvila, Cesar LuisTorres Bugeau, Clarisa Maria2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/252701Surface behavior of ceramides with very long chain polyunsaturated fatty acids; XLI Reunión Anual de la Sociedad Argentina de Biofísica; Tucumán; Argentina; 2012; 199-199978-987-27591-1-7CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:46Zoai:ri.conicet.gov.ar:11336/252701instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:46.28CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Surface behavior of ceramides with very long chain polyunsaturated fatty acids
title Surface behavior of ceramides with very long chain polyunsaturated fatty acids
spellingShingle Surface behavior of ceramides with very long chain polyunsaturated fatty acids
Peñalva, Daniel Alejandro
CERAMIDE
SPHINGOLIPIDS
LANGMUIR MONOLAYER
VLCPUFA
title_short Surface behavior of ceramides with very long chain polyunsaturated fatty acids
title_full Surface behavior of ceramides with very long chain polyunsaturated fatty acids
title_fullStr Surface behavior of ceramides with very long chain polyunsaturated fatty acids
title_full_unstemmed Surface behavior of ceramides with very long chain polyunsaturated fatty acids
title_sort Surface behavior of ceramides with very long chain polyunsaturated fatty acids
dc.creator.none.fl_str_mv Peñalva, Daniel Alejandro
Fanani, Maria Laura
Oresti, Gerardo Martin
Antollini, Silvia Susana
Maggio, Bruno
Aveldaño, Marta Isabel
author Peñalva, Daniel Alejandro
author_facet Peñalva, Daniel Alejandro
Fanani, Maria Laura
Oresti, Gerardo Martin
Antollini, Silvia Susana
Maggio, Bruno
Aveldaño, Marta Isabel
author_role author
author2 Fanani, Maria Laura
Oresti, Gerardo Martin
Antollini, Silvia Susana
Maggio, Bruno
Aveldaño, Marta Isabel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Chehin, Rosana Nieves
Avila, Cesar Luis
Torres Bugeau, Clarisa Maria
dc.subject.none.fl_str_mv CERAMIDE
SPHINGOLIPIDS
LANGMUIR MONOLAYER
VLCPUFA
topic CERAMIDE
SPHINGOLIPIDS
LANGMUIR MONOLAYER
VLCPUFA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Molecular species of sphingomyelin (SM) and ceramide (Cer) with nonhydroxy and 2-hydroxy very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) abound in mammalian spermatogenic cells and spermatozoa. In rat sperm, Cer naturally occurs in the tail and is produced from SM in the head once the acrosomal reaction is completed. The properties these atypical lipids confer to membranes remain to be defined. The aim of this study was to evaluate surface properties of isolated molecular species of Cer in monolayers. Ceramides containing nonhydroxy and 2-hydroxy fatty acids like 28:4, 30:5 and 32:5 isolated from rat testes, were resolved by a combination of chromatographic techniques including HPLC. Langmuir monolayer studies allowed to measure surface pressure, surface potential and reflectivity images by Brewster angle microscopy of these Cer spread at the air-water interface. We were able to determine average molecular area (degree of packing), surface potential, and thickness of these films. Isotherms of these molecular species showed expanded phases at large molecular areas and in-plane phase transitions to a liquid condensed state at lower molecular areas at both 7°C and 21°C. In the transition region, the formation of domains with different shapes could be appreciated. Our results show that ceramide surface properties depend on fatty acid unsaturation, chain length and the presence of the 2-hydroxyl group in the acyl chain. Furthermore, most of these properties differed significantly from those of previously characterized Cer with long-chain saturated fatty acids.
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fanani, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLI Reunión Anual de la Sociedad Argentina de Biofísica
Tucumán
Argentina
Sociedad Argentina de Biofísica
description Molecular species of sphingomyelin (SM) and ceramide (Cer) with nonhydroxy and 2-hydroxy very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) abound in mammalian spermatogenic cells and spermatozoa. In rat sperm, Cer naturally occurs in the tail and is produced from SM in the head once the acrosomal reaction is completed. The properties these atypical lipids confer to membranes remain to be defined. The aim of this study was to evaluate surface properties of isolated molecular species of Cer in monolayers. Ceramides containing nonhydroxy and 2-hydroxy fatty acids like 28:4, 30:5 and 32:5 isolated from rat testes, were resolved by a combination of chromatographic techniques including HPLC. Langmuir monolayer studies allowed to measure surface pressure, surface potential and reflectivity images by Brewster angle microscopy of these Cer spread at the air-water interface. We were able to determine average molecular area (degree of packing), surface potential, and thickness of these films. Isotherms of these molecular species showed expanded phases at large molecular areas and in-plane phase transitions to a liquid condensed state at lower molecular areas at both 7°C and 21°C. In the transition region, the formation of domains with different shapes could be appreciated. Our results show that ceramide surface properties depend on fatty acid unsaturation, chain length and the presence of the 2-hydroxyl group in the acyl chain. Furthermore, most of these properties differed significantly from those of previously characterized Cer with long-chain saturated fatty acids.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/252701
Surface behavior of ceramides with very long chain polyunsaturated fatty acids; XLI Reunión Anual de la Sociedad Argentina de Biofísica; Tucumán; Argentina; 2012; 199-199
978-987-27591-1-7
CONICET Digital
CONICET
url http://hdl.handle.net/11336/252701
identifier_str_mv Surface behavior of ceramides with very long chain polyunsaturated fatty acids; XLI Reunión Anual de la Sociedad Argentina de Biofísica; Tucumán; Argentina; 2012; 199-199
978-987-27591-1-7
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613226217603072
score 13.070432

Publicaciones similares