Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid

Autores
Oresti, Gerardo Martin; Luquez, Jessica Mariela; Belmonte, Silvia Alejandra
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sperm membrane lipids are crucial in male fertility, influencing sperm motility, viability, and functional competence. This study comprehensively characterizes the phospholipid and sphingolipid composition in highly motile human spermatozoa obtained through the swim-up method, a widely used technique in assisted reproductive technology (ART). Using two-dimensional thin-layer chromatography and phosphorus analysis, we identified choline glycerophospholipids (CGP, 45%), ethanolamine glycerophospholipids (EGP, 26%), and sphingomyelin (SM, 17%) as predominant phospholipids, with minor components including cardiolipin, lysophospholipids, phosphatidylinositol, phosphatidylserine, phosphatidic acid, and neutral lipids. Gas chromatography analysis of glycerophospholipids (GPL) revealed a high long chain (C20-C22) polyunsaturated fatty acid (PUFA) content (46.3%), particularly docosahexaenoic acid (DHA, 29 22:6n-3), which was more abundant in CGP (46%) than EGP (26%). Sphingolipid analysis 30 indicated that ceramide (Cer) and SM shared similar fatty acid profiles due to their metabolic relationship, with very-long-chain (VLC) PUFA (≥C26) being more prevalent in SM (10%) than in Cer (6%). Additionally, argentation chromatography allowed the identification of highly unsaturated VLCPUFA species in Cer, including 28:3n-6, 28:4n-6, and 30:4n-34 6, which had not been previously quantified in motile human spermatozoa. Given the essential function of sphingolipid metabolism in spermatogenesis, capacitation, and acrosomal exocytosis, our findings suggest that the balance of VLCPUFA-containing SM and Cer emerges as a key factor in sperm performance and fertilization potential. This study provides novel insights into the lipid signature of human sperm and highlights the relevance of membrane lipid remodeling for male fertility and ART outcomes.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Belmonte, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
Materia
SPHINGOLIPIDS
CERAMIDE
SPHINGOMYELIN
PHOSPHOLIPIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261400
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acidOresti, Gerardo MartinLuquez, Jessica MarielaBelmonte, Silvia AlejandraSPHINGOLIPIDSCERAMIDESPHINGOMYELINPHOSPHOLIPIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sperm membrane lipids are crucial in male fertility, influencing sperm motility, viability, and functional competence. This study comprehensively characterizes the phospholipid and sphingolipid composition in highly motile human spermatozoa obtained through the swim-up method, a widely used technique in assisted reproductive technology (ART). Using two-dimensional thin-layer chromatography and phosphorus analysis, we identified choline glycerophospholipids (CGP, 45%), ethanolamine glycerophospholipids (EGP, 26%), and sphingomyelin (SM, 17%) as predominant phospholipids, with minor components including cardiolipin, lysophospholipids, phosphatidylinositol, phosphatidylserine, phosphatidic acid, and neutral lipids. Gas chromatography analysis of glycerophospholipids (GPL) revealed a high long chain (C20-C22) polyunsaturated fatty acid (PUFA) content (46.3%), particularly docosahexaenoic acid (DHA, 29 22:6n-3), which was more abundant in CGP (46%) than EGP (26%). Sphingolipid analysis 30 indicated that ceramide (Cer) and SM shared similar fatty acid profiles due to their metabolic relationship, with very-long-chain (VLC) PUFA (≥C26) being more prevalent in SM (10%) than in Cer (6%). Additionally, argentation chromatography allowed the identification of highly unsaturated VLCPUFA species in Cer, including 28:3n-6, 28:4n-6, and 30:4n-34 6, which had not been previously quantified in motile human spermatozoa. Given the essential function of sphingolipid metabolism in spermatogenesis, capacitation, and acrosomal exocytosis, our findings suggest that the balance of VLCPUFA-containing SM and Cer emerges as a key factor in sperm performance and fertilization potential. This study provides novel insights into the lipid signature of human sperm and highlights the relevance of membrane lipid remodeling for male fertility and ART outcomes.Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Belmonte, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaCold Spring Harbor Laboratory Press2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261400Oresti, Gerardo Martin; Luquez, Jessica Mariela; Belmonte, Silvia Alejandra; Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid; Cold Spring Harbor Laboratory Press; BioRxiv; 2025; 2-2025; 1-142692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2025.02.17.638566v1info:eu-repo/semantics/altIdentifier/doi/10.1101/2025.02.17.638566info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:10:40Zoai:ri.conicet.gov.ar:11336/261400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:10:40.337CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
title Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
spellingShingle Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
Oresti, Gerardo Martin
SPHINGOLIPIDS
CERAMIDE
SPHINGOMYELIN
PHOSPHOLIPIDS
title_short Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
title_full Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
title_fullStr Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
title_full_unstemmed Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
title_sort Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Luquez, Jessica Mariela
Belmonte, Silvia Alejandra
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Luquez, Jessica Mariela
Belmonte, Silvia Alejandra
author_role author
author2 Luquez, Jessica Mariela
Belmonte, Silvia Alejandra
author2_role author
author
dc.subject.none.fl_str_mv SPHINGOLIPIDS
CERAMIDE
SPHINGOMYELIN
PHOSPHOLIPIDS
topic SPHINGOLIPIDS
CERAMIDE
SPHINGOMYELIN
PHOSPHOLIPIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sperm membrane lipids are crucial in male fertility, influencing sperm motility, viability, and functional competence. This study comprehensively characterizes the phospholipid and sphingolipid composition in highly motile human spermatozoa obtained through the swim-up method, a widely used technique in assisted reproductive technology (ART). Using two-dimensional thin-layer chromatography and phosphorus analysis, we identified choline glycerophospholipids (CGP, 45%), ethanolamine glycerophospholipids (EGP, 26%), and sphingomyelin (SM, 17%) as predominant phospholipids, with minor components including cardiolipin, lysophospholipids, phosphatidylinositol, phosphatidylserine, phosphatidic acid, and neutral lipids. Gas chromatography analysis of glycerophospholipids (GPL) revealed a high long chain (C20-C22) polyunsaturated fatty acid (PUFA) content (46.3%), particularly docosahexaenoic acid (DHA, 29 22:6n-3), which was more abundant in CGP (46%) than EGP (26%). Sphingolipid analysis 30 indicated that ceramide (Cer) and SM shared similar fatty acid profiles due to their metabolic relationship, with very-long-chain (VLC) PUFA (≥C26) being more prevalent in SM (10%) than in Cer (6%). Additionally, argentation chromatography allowed the identification of highly unsaturated VLCPUFA species in Cer, including 28:3n-6, 28:4n-6, and 30:4n-34 6, which had not been previously quantified in motile human spermatozoa. Given the essential function of sphingolipid metabolism in spermatogenesis, capacitation, and acrosomal exocytosis, our findings suggest that the balance of VLCPUFA-containing SM and Cer emerges as a key factor in sperm performance and fertilization potential. This study provides novel insights into the lipid signature of human sperm and highlights the relevance of membrane lipid remodeling for male fertility and ART outcomes.
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Luquez, Jessica Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Belmonte, Silvia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
description Sperm membrane lipids are crucial in male fertility, influencing sperm motility, viability, and functional competence. This study comprehensively characterizes the phospholipid and sphingolipid composition in highly motile human spermatozoa obtained through the swim-up method, a widely used technique in assisted reproductive technology (ART). Using two-dimensional thin-layer chromatography and phosphorus analysis, we identified choline glycerophospholipids (CGP, 45%), ethanolamine glycerophospholipids (EGP, 26%), and sphingomyelin (SM, 17%) as predominant phospholipids, with minor components including cardiolipin, lysophospholipids, phosphatidylinositol, phosphatidylserine, phosphatidic acid, and neutral lipids. Gas chromatography analysis of glycerophospholipids (GPL) revealed a high long chain (C20-C22) polyunsaturated fatty acid (PUFA) content (46.3%), particularly docosahexaenoic acid (DHA, 29 22:6n-3), which was more abundant in CGP (46%) than EGP (26%). Sphingolipid analysis 30 indicated that ceramide (Cer) and SM shared similar fatty acid profiles due to their metabolic relationship, with very-long-chain (VLC) PUFA (≥C26) being more prevalent in SM (10%) than in Cer (6%). Additionally, argentation chromatography allowed the identification of highly unsaturated VLCPUFA species in Cer, including 28:3n-6, 28:4n-6, and 30:4n-34 6, which had not been previously quantified in motile human spermatozoa. Given the essential function of sphingolipid metabolism in spermatogenesis, capacitation, and acrosomal exocytosis, our findings suggest that the balance of VLCPUFA-containing SM and Cer emerges as a key factor in sperm performance and fertilization potential. This study provides novel insights into the lipid signature of human sperm and highlights the relevance of membrane lipid remodeling for male fertility and ART outcomes.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261400
Oresti, Gerardo Martin; Luquez, Jessica Mariela; Belmonte, Silvia Alejandra; Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid; Cold Spring Harbor Laboratory Press; BioRxiv; 2025; 2-2025; 1-14
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261400
identifier_str_mv Oresti, Gerardo Martin; Luquez, Jessica Mariela; Belmonte, Silvia Alejandra; Lipid signature of motile human sperm: Characterization of sphingomyelin, ceramide, and phospholipids with a focus on very long polyunsaturated fatty acid; Cold Spring Harbor Laboratory Press; BioRxiv; 2025; 2-2025; 1-14
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2025.02.17.638566v1
info:eu-repo/semantics/altIdentifier/doi/10.1101/2025.02.17.638566
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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