Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis

Autores
Peñalva, Daniel Alejandro; Fanani, M.L.; Maggio, Bruno; Aveldaño, Marta Isabel; Antollini, Silvia Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) are important acyl groups of sphingomyelin (SM) and ceramide (Cer) of mammalian spermatozoa. In rat sperm, SM species containing PUFA with 28-32 carbon atoms are exclusively located on the heads. After inducing the acrosomal reaction almost complete hydrolysis such SMs occurs, leading to gametes considerably enriched in the corresponding Cer species. The aim of this study was to evaluate the effects of the sphingomyelinaseinduced conversion VLCPUFA-SM → VLCPUFA-Cer on the biophysical properties of a binary model system (POPC/SM). The VLCPUFA-containing molecular species of SM were isolated from rat testes by a combination of chromatographic techniques. Egg-SM, whose properties are widely known, was used for comparison. Unilamellar liposomes (LUVs) were prepared and Dynamic Light Scattering was used to evaluate their structures/sizes before and after enzymatic hydrolysis. The potential increase in the interaction between different populations of liposomes (fission/fussion) after the SM → Cer hydrolysis was evaluated by FRET assays using NBD-PE as donor and Rh-PE as acceptor, and the lateral segregation of phases after Cer generation was followed by anisotropy of different fluorescence probes (laurdan, DPH and NBD-PE). In all biophysical properties measured, the SM species containing VLCPUFA contrasted with those of the egg-derived saturated SM. The longer and bulkier acyl chains of VLCPUFA-Cer may play a role in favouring the fusion/fission events that occur in the head of spermatozoa during the acrosomal reaction, a process that requires topological lipid intermediates with negative curvature
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fanani, M.L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLII Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
Materia
SPHINGOMYELIN
CERAMIDE
BIOPHYSICAL PROPERTIES
FUSSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231861
Acceder
id CONICETDig_a3cb87f1878f5aa4f3c0bad1a46b3362
oai_identifier_str oai:ri.conicet.gov.ar:11336/231861
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysisPeñalva, Daniel AlejandroFanani, M.L.Maggio, BrunoAveldaño, Marta IsabelAntollini, Silvia SusanaSPHINGOMYELINCERAMIDEBIOPHYSICAL PROPERTIESFUSSIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) are important acyl groups of sphingomyelin (SM) and ceramide (Cer) of mammalian spermatozoa. In rat sperm, SM species containing PUFA with 28-32 carbon atoms are exclusively located on the heads. After inducing the acrosomal reaction almost complete hydrolysis such SMs occurs, leading to gametes considerably enriched in the corresponding Cer species. The aim of this study was to evaluate the effects of the sphingomyelinaseinduced conversion VLCPUFA-SM → VLCPUFA-Cer on the biophysical properties of a binary model system (POPC/SM). The VLCPUFA-containing molecular species of SM were isolated from rat testes by a combination of chromatographic techniques. Egg-SM, whose properties are widely known, was used for comparison. Unilamellar liposomes (LUVs) were prepared and Dynamic Light Scattering was used to evaluate their structures/sizes before and after enzymatic hydrolysis. The potential increase in the interaction between different populations of liposomes (fission/fussion) after the SM → Cer hydrolysis was evaluated by FRET assays using NBD-PE as donor and Rh-PE as acceptor, and the lateral segregation of phases after Cer generation was followed by anisotropy of different fluorescence probes (laurdan, DPH and NBD-PE). In all biophysical properties measured, the SM species containing VLCPUFA contrasted with those of the egg-derived saturated SM. The longer and bulkier acyl chains of VLCPUFA-Cer may play a role in favouring the fusion/fission events that occur in the head of spermatozoa during the acrosomal reaction, a process that requires topological lipid intermediates with negative curvatureFil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Fanani, M.L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLII Reunión Anual de la Sociedad Argentina de BiofísicaCórdobaArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231861Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2013; 40-40978-987-27591-2-4CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:38Zoai:ri.conicet.gov.ar:11336/231861instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:38.791CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
title Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
spellingShingle Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
Peñalva, Daniel Alejandro
SPHINGOMYELIN
CERAMIDE
BIOPHYSICAL PROPERTIES
FUSSION
title_short Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
title_full Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
title_fullStr Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
title_full_unstemmed Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
title_sort Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis
dc.creator.none.fl_str_mv Peñalva, Daniel Alejandro
Fanani, M.L.
Maggio, Bruno
Aveldaño, Marta Isabel
Antollini, Silvia Susana
author Peñalva, Daniel Alejandro
author_facet Peñalva, Daniel Alejandro
Fanani, M.L.
Maggio, Bruno
Aveldaño, Marta Isabel
Antollini, Silvia Susana
author_role author
author2 Fanani, M.L.
Maggio, Bruno
Aveldaño, Marta Isabel
Antollini, Silvia Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv SPHINGOMYELIN
CERAMIDE
BIOPHYSICAL PROPERTIES
FUSSION
topic SPHINGOMYELIN
CERAMIDE
BIOPHYSICAL PROPERTIES
FUSSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) are important acyl groups of sphingomyelin (SM) and ceramide (Cer) of mammalian spermatozoa. In rat sperm, SM species containing PUFA with 28-32 carbon atoms are exclusively located on the heads. After inducing the acrosomal reaction almost complete hydrolysis such SMs occurs, leading to gametes considerably enriched in the corresponding Cer species. The aim of this study was to evaluate the effects of the sphingomyelinaseinduced conversion VLCPUFA-SM → VLCPUFA-Cer on the biophysical properties of a binary model system (POPC/SM). The VLCPUFA-containing molecular species of SM were isolated from rat testes by a combination of chromatographic techniques. Egg-SM, whose properties are widely known, was used for comparison. Unilamellar liposomes (LUVs) were prepared and Dynamic Light Scattering was used to evaluate their structures/sizes before and after enzymatic hydrolysis. The potential increase in the interaction between different populations of liposomes (fission/fussion) after the SM → Cer hydrolysis was evaluated by FRET assays using NBD-PE as donor and Rh-PE as acceptor, and the lateral segregation of phases after Cer generation was followed by anisotropy of different fluorescence probes (laurdan, DPH and NBD-PE). In all biophysical properties measured, the SM species containing VLCPUFA contrasted with those of the egg-derived saturated SM. The longer and bulkier acyl chains of VLCPUFA-Cer may play a role in favouring the fusion/fission events that occur in the head of spermatozoa during the acrosomal reaction, a process that requires topological lipid intermediates with negative curvature
Fil: Peñalva, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Fanani, M.L.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Maggio, Bruno. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Antollini, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLII Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
description Very long-chain (C24 to C36) polyunsaturated fatty acids (VLCPUFA) are important acyl groups of sphingomyelin (SM) and ceramide (Cer) of mammalian spermatozoa. In rat sperm, SM species containing PUFA with 28-32 carbon atoms are exclusively located on the heads. After inducing the acrosomal reaction almost complete hydrolysis such SMs occurs, leading to gametes considerably enriched in the corresponding Cer species. The aim of this study was to evaluate the effects of the sphingomyelinaseinduced conversion VLCPUFA-SM → VLCPUFA-Cer on the biophysical properties of a binary model system (POPC/SM). The VLCPUFA-containing molecular species of SM were isolated from rat testes by a combination of chromatographic techniques. Egg-SM, whose properties are widely known, was used for comparison. Unilamellar liposomes (LUVs) were prepared and Dynamic Light Scattering was used to evaluate their structures/sizes before and after enzymatic hydrolysis. The potential increase in the interaction between different populations of liposomes (fission/fussion) after the SM → Cer hydrolysis was evaluated by FRET assays using NBD-PE as donor and Rh-PE as acceptor, and the lateral segregation of phases after Cer generation was followed by anisotropy of different fluorescence probes (laurdan, DPH and NBD-PE). In all biophysical properties measured, the SM species containing VLCPUFA contrasted with those of the egg-derived saturated SM. The longer and bulkier acyl chains of VLCPUFA-Cer may play a role in favouring the fusion/fission events that occur in the head of spermatozoa during the acrosomal reaction, a process that requires topological lipid intermediates with negative curvature
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231861
Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2013; 40-40
978-987-27591-2-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231861
identifier_str_mv Changes in biophysical properties of membranes containing sphingomyelin with very long chain PUFA induced by its hydrolysis; XLII Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2013; 40-40
978-987-27591-2-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/reuniones-cientificas/reunionsab-previas/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083512161009664
score 13.22299

Publicaciones similares