Tig1 regulates proximo-distal identity during salamander limb regeneration

Autores
Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; Gates, Phillip B.; Walters, Hannah E.; Petzold, Andreas; Arce, Hernán; Cordoba, Rodrigo C.; Subramanian, Elaiyaraja; Chara, Osvaldo; Tanaka, Elly M.; Simon, András; Yun, Maximina H.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
Fil: Oliveira, Catarina R.. Technische Universität Dresden; Alemania
Fil: Knapp, Dunja. Technische Universität Dresden; Alemania
Fil: Elewa, Ahmed. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Gerber, Tobias. European Molecular Biology Laboratory; Alemania
Fil: Gonzalez Malagon, Sandra G.. University of Ioannina; Reino Unido. Colegio Universitario de Londres; Reino Unido
Fil: Gates, Phillip B.. Colegio Universitario de Londres; Reino Unido
Fil: Walters, Hannah E.. Technische Universität Dresden; Alemania
Fil: Petzold, Andreas. Technische Universität Dresden; Alemania
Fil: Arce, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina
Fil: Cordoba, Rodrigo C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Subramanian, Elaiyaraja. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; Alemania
Fil: Tanaka, Elly M.. Research Institute Of Molecular Pathology; Austria
Fil: Simon, András. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Yun, Maximina H.. Max Planck Institute Of Molecular Cell Biology And Genetics; Alemania. Technische Universität Dresden; Alemania
Materia
AXOLOTL
TIG1
PROXIMAL IDENTITY
REGENERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/212654
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/212654
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tig1 regulates proximo-distal identity during salamander limb regenerationOliveira, Catarina R.Knapp, DunjaElewa, AhmedGerber, TobiasGonzalez Malagon, Sandra G.Gates, Phillip B.Walters, Hannah E.Petzold, AndreasArce, HernánCordoba, Rodrigo C.Subramanian, ElaiyarajaChara, OsvaldoTanaka, Elly M.Simon, AndrásYun, Maximina H.AXOLOTLTIG1PROXIMAL IDENTITYREGENERATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.Fil: Oliveira, Catarina R.. Technische Universität Dresden; AlemaniaFil: Knapp, Dunja. Technische Universität Dresden; AlemaniaFil: Elewa, Ahmed. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Gerber, Tobias. European Molecular Biology Laboratory; AlemaniaFil: Gonzalez Malagon, Sandra G.. University of Ioannina; Reino Unido. Colegio Universitario de Londres; Reino UnidoFil: Gates, Phillip B.. Colegio Universitario de Londres; Reino UnidoFil: Walters, Hannah E.. Technische Universität Dresden; AlemaniaFil: Petzold, Andreas. Technische Universität Dresden; AlemaniaFil: Arce, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; ArgentinaFil: Cordoba, Rodrigo C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; ArgentinaFil: Subramanian, Elaiyaraja. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; AlemaniaFil: Tanaka, Elly M.. Research Institute Of Molecular Pathology; AustriaFil: Simon, András. Karolinska Huddinge Hospital. Karolinska Institutet; SueciaFil: Yun, Maximina H.. Max Planck Institute Of Molecular Cell Biology And Genetics; Alemania. Technische Universität Dresden; AlemaniaNature Research2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/212654Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; et al.; Tig1 regulates proximo-distal identity during salamander limb regeneration; Nature Research; Nature Communications; 13; 1; 12-2022; 1-162041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-022-28755-1info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-022-28755-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:33:26Zoai:ri.conicet.gov.ar:11336/212654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:33:26.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tig1 regulates proximo-distal identity during salamander limb regeneration
title Tig1 regulates proximo-distal identity during salamander limb regeneration
spellingShingle Tig1 regulates proximo-distal identity during salamander limb regeneration
Oliveira, Catarina R.
AXOLOTL
TIG1
PROXIMAL IDENTITY
REGENERATION
title_short Tig1 regulates proximo-distal identity during salamander limb regeneration
title_full Tig1 regulates proximo-distal identity during salamander limb regeneration
title_fullStr Tig1 regulates proximo-distal identity during salamander limb regeneration
title_full_unstemmed Tig1 regulates proximo-distal identity during salamander limb regeneration
title_sort Tig1 regulates proximo-distal identity during salamander limb regeneration
dc.creator.none.fl_str_mv Oliveira, Catarina R.
Knapp, Dunja
Elewa, Ahmed
Gerber, Tobias
Gonzalez Malagon, Sandra G.
Gates, Phillip B.
Walters, Hannah E.
Petzold, Andreas
Arce, Hernán
Cordoba, Rodrigo C.
Subramanian, Elaiyaraja
Chara, Osvaldo
Tanaka, Elly M.
Simon, András
Yun, Maximina H.
author Oliveira, Catarina R.
author_facet Oliveira, Catarina R.
Knapp, Dunja
Elewa, Ahmed
Gerber, Tobias
Gonzalez Malagon, Sandra G.
Gates, Phillip B.
Walters, Hannah E.
Petzold, Andreas
Arce, Hernán
Cordoba, Rodrigo C.
Subramanian, Elaiyaraja
Chara, Osvaldo
Tanaka, Elly M.
Simon, András
Yun, Maximina H.
author_role author
author2 Knapp, Dunja
Elewa, Ahmed
Gerber, Tobias
Gonzalez Malagon, Sandra G.
Gates, Phillip B.
Walters, Hannah E.
Petzold, Andreas
Arce, Hernán
Cordoba, Rodrigo C.
Subramanian, Elaiyaraja
Chara, Osvaldo
Tanaka, Elly M.
Simon, András
Yun, Maximina H.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AXOLOTL
TIG1
PROXIMAL IDENTITY
REGENERATION
topic AXOLOTL
TIG1
PROXIMAL IDENTITY
REGENERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
Fil: Oliveira, Catarina R.. Technische Universität Dresden; Alemania
Fil: Knapp, Dunja. Technische Universität Dresden; Alemania
Fil: Elewa, Ahmed. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Gerber, Tobias. European Molecular Biology Laboratory; Alemania
Fil: Gonzalez Malagon, Sandra G.. University of Ioannina; Reino Unido. Colegio Universitario de Londres; Reino Unido
Fil: Gates, Phillip B.. Colegio Universitario de Londres; Reino Unido
Fil: Walters, Hannah E.. Technische Universität Dresden; Alemania
Fil: Petzold, Andreas. Technische Universität Dresden; Alemania
Fil: Arce, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina
Fil: Cordoba, Rodrigo C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina
Fil: Subramanian, Elaiyaraja. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Argentina de la Empresa; Argentina. Technische Universität Dresden; Alemania
Fil: Tanaka, Elly M.. Research Institute Of Molecular Pathology; Austria
Fil: Simon, András. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
Fil: Yun, Maximina H.. Max Planck Institute Of Molecular Cell Biology And Genetics; Alemania. Technische Universität Dresden; Alemania
description Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/212654
Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; et al.; Tig1 regulates proximo-distal identity during salamander limb regeneration; Nature Research; Nature Communications; 13; 1; 12-2022; 1-16
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/212654
identifier_str_mv Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; et al.; Tig1 regulates proximo-distal identity during salamander limb regeneration; Nature Research; Nature Communications; 13; 1; 12-2022; 1-16
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-022-28755-1
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-022-28755-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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