Progressive specification rather than intercalation of segments during limb regeneration

Autores
Roensch, Kathleen; Tazaky, Akira; Chara, Osvaldo; Tanaka, Elly M.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An amputated salamander limb regenerates the correct number of segments. Models explaining limb regeneration were largely distinct from those for limb development, despite the presence of common patterning molecules. Intercalation has been an important concept to explain salamander limb regeneration, but clear evidence supporting or refuting this model was lacking. In the intercalation model, the first blastema cells acquire fingertip identity, creating a gap in positional identity that triggers regeneration of the intervening region from the stump. We used HOXA protein analysis and transplantation assays to show that axolotl limb blastema cells acquire positional identity in a proximal-to-distal sequence. Therefore, intercalation is not the primary mechanism for segment formation during limb regeneration in this animal. Patterning in development and regeneration uses similar mechanisms.
Fil: Roensch, Kathleen. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Tazaky, Akira. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; Alemania
Fil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Materia
Regeneration
Intercalation
Axolotl
Progresive Specification
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23647
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Progressive specification rather than intercalation of segments during limb regenerationRoensch, KathleenTazaky, AkiraChara, OsvaldoTanaka, Elly M.RegenerationIntercalationAxolotlProgresive Specificationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An amputated salamander limb regenerates the correct number of segments. Models explaining limb regeneration were largely distinct from those for limb development, despite the presence of common patterning molecules. Intercalation has been an important concept to explain salamander limb regeneration, but clear evidence supporting or refuting this model was lacking. In the intercalation model, the first blastema cells acquire fingertip identity, creating a gap in positional identity that triggers regeneration of the intervening region from the stump. We used HOXA protein analysis and transplantation assays to show that axolotl limb blastema cells acquire positional identity in a proximal-to-distal sequence. Therefore, intercalation is not the primary mechanism for segment formation during limb regeneration in this animal. Patterning in development and regeneration uses similar mechanisms.Fil: Roensch, Kathleen. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; AlemaniaFil: Tazaky, Akira. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; AlemaniaFil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; AlemaniaFil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; AlemaniaAmerican Association for the Advancement of Science2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23647Roensch, Kathleen; Tazaky, Akira; Chara, Osvaldo; Tanaka, Elly M.; Progressive specification rather than intercalation of segments during limb regeneration; American Association for the Advancement of Science; Science; 342; 6164; 12-2013; 1375-13790036-8075CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1126/science.1241796info:eu-repo/semantics/altIdentifier/url/http://science.sciencemag.org/content/342/6164/1375info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:05:45Zoai:ri.conicet.gov.ar:11336/23647instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:05:45.893CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Progressive specification rather than intercalation of segments during limb regeneration
title Progressive specification rather than intercalation of segments during limb regeneration
spellingShingle Progressive specification rather than intercalation of segments during limb regeneration
Roensch, Kathleen
Regeneration
Intercalation
Axolotl
Progresive Specification
title_short Progressive specification rather than intercalation of segments during limb regeneration
title_full Progressive specification rather than intercalation of segments during limb regeneration
title_fullStr Progressive specification rather than intercalation of segments during limb regeneration
title_full_unstemmed Progressive specification rather than intercalation of segments during limb regeneration
title_sort Progressive specification rather than intercalation of segments during limb regeneration
dc.creator.none.fl_str_mv Roensch, Kathleen
Tazaky, Akira
Chara, Osvaldo
Tanaka, Elly M.
author Roensch, Kathleen
author_facet Roensch, Kathleen
Tazaky, Akira
Chara, Osvaldo
Tanaka, Elly M.
author_role author
author2 Tazaky, Akira
Chara, Osvaldo
Tanaka, Elly M.
author2_role author
author
author
dc.subject.none.fl_str_mv Regeneration
Intercalation
Axolotl
Progresive Specification
topic Regeneration
Intercalation
Axolotl
Progresive Specification
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An amputated salamander limb regenerates the correct number of segments. Models explaining limb regeneration were largely distinct from those for limb development, despite the presence of common patterning molecules. Intercalation has been an important concept to explain salamander limb regeneration, but clear evidence supporting or refuting this model was lacking. In the intercalation model, the first blastema cells acquire fingertip identity, creating a gap in positional identity that triggers regeneration of the intervening region from the stump. We used HOXA protein analysis and transplantation assays to show that axolotl limb blastema cells acquire positional identity in a proximal-to-distal sequence. Therefore, intercalation is not the primary mechanism for segment formation during limb regeneration in this animal. Patterning in development and regeneration uses similar mechanisms.
Fil: Roensch, Kathleen. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Tazaky, Akira. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universität Dresden; Alemania
Fil: Tanaka, Elly M.. Technische Universität Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
description An amputated salamander limb regenerates the correct number of segments. Models explaining limb regeneration were largely distinct from those for limb development, despite the presence of common patterning molecules. Intercalation has been an important concept to explain salamander limb regeneration, but clear evidence supporting or refuting this model was lacking. In the intercalation model, the first blastema cells acquire fingertip identity, creating a gap in positional identity that triggers regeneration of the intervening region from the stump. We used HOXA protein analysis and transplantation assays to show that axolotl limb blastema cells acquire positional identity in a proximal-to-distal sequence. Therefore, intercalation is not the primary mechanism for segment formation during limb regeneration in this animal. Patterning in development and regeneration uses similar mechanisms.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23647
Roensch, Kathleen; Tazaky, Akira; Chara, Osvaldo; Tanaka, Elly M.; Progressive specification rather than intercalation of segments during limb regeneration; American Association for the Advancement of Science; Science; 342; 6164; 12-2013; 1375-1379
0036-8075
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23647
identifier_str_mv Roensch, Kathleen; Tazaky, Akira; Chara, Osvaldo; Tanaka, Elly M.; Progressive specification rather than intercalation of segments during limb regeneration; American Association for the Advancement of Science; Science; 342; 6164; 12-2013; 1375-1379
0036-8075
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1126/science.1241796
info:eu-repo/semantics/altIdentifier/url/http://science.sciencemag.org/content/342/6164/1375
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for the Advancement of Science
publisher.none.fl_str_mv American Association for the Advancement of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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