Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells

Autores
Villena, Julio Cesar; Suzuki, Rie; Fujie, Hitomi; Chiba, Eriko; Takahashi, Takuya; Tomosada, Yohsuke; Shimazu, Tomoyuki; Aso, Hisashi; Ohwada, Shuichi; Suda, Yoshihito; Ikegami, Shuji; Itoh, Hiroyuki; Alvarez, Gladis Susana; Saito, Tadao; Kitazawa, Haruki
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Suzuki, Rie. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Takahashi, Takuya. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Tomosada, Yohsuke. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimazu, Tomoyuki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Ohwada, Shuichi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Saito, Tadao. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Kitazawa, Haruki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Materia
Lactobacillus Jensenii
Porcine Antigen Presenting Cells
Anti-Inflammatory Activity
Toll-Like Receptor 4
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24931
Acceder
id CONICETDig_ef5fe7ad9fc3c964cefcfae2e09665db
oai_identifier_str oai:ri.conicet.gov.ar:11336/24931
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cellsVillena, Julio CesarSuzuki, RieFujie, HitomiChiba, ErikoTakahashi, TakuyaTomosada, YohsukeShimazu, TomoyukiAso, HisashiOhwada, ShuichiSuda, YoshihitoIkegami, ShujiItoh, HiroyukiAlvarez, Gladis SusanaSaito, TadaoKitazawa, HarukiLactobacillus JenseniiPorcine Antigen Presenting CellsAnti-Inflammatory ActivityToll-Like Receptor 4https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Suzuki, Rie. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Fujie, Hitomi. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Chiba, Eriko. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Takahashi, Takuya. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Tomosada, Yohsuke. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Shimazu, Tomoyuki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Ohwada, Shuichi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; JapónFil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; JapónFil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; JapónFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Saito, Tadao. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Kitazawa, Haruki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; JapónAmerican Society for Microbiology2012-05-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24931Villena, Julio Cesar; Suzuki, Rie; Fujie, Hitomi; Chiba, Eriko; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells; American Society for Microbiology; Clinical And Vaccine Immunology; 19; 7; 9-5-2012; 1038-10531556-68111556-679XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/CVI.00199-12info:eu-repo/semantics/altIdentifier/url/http://cvi.asm.org/content/19/7/1038info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:37Zoai:ri.conicet.gov.ar:11336/24931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:38.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
title Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
spellingShingle Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
Villena, Julio Cesar
Lactobacillus Jensenii
Porcine Antigen Presenting Cells
Anti-Inflammatory Activity
Toll-Like Receptor 4
title_short Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
title_full Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
title_fullStr Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
title_full_unstemmed Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
title_sort Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells
dc.creator.none.fl_str_mv Villena, Julio Cesar
Suzuki, Rie
Fujie, Hitomi
Chiba, Eriko
Takahashi, Takuya
Tomosada, Yohsuke
Shimazu, Tomoyuki
Aso, Hisashi
Ohwada, Shuichi
Suda, Yoshihito
Ikegami, Shuji
Itoh, Hiroyuki
Alvarez, Gladis Susana
Saito, Tadao
Kitazawa, Haruki
author Villena, Julio Cesar
author_facet Villena, Julio Cesar
Suzuki, Rie
Fujie, Hitomi
Chiba, Eriko
Takahashi, Takuya
Tomosada, Yohsuke
Shimazu, Tomoyuki
Aso, Hisashi
Ohwada, Shuichi
Suda, Yoshihito
Ikegami, Shuji
Itoh, Hiroyuki
Alvarez, Gladis Susana
Saito, Tadao
Kitazawa, Haruki
author_role author
author2 Suzuki, Rie
Fujie, Hitomi
Chiba, Eriko
Takahashi, Takuya
Tomosada, Yohsuke
Shimazu, Tomoyuki
Aso, Hisashi
Ohwada, Shuichi
Suda, Yoshihito
Ikegami, Shuji
Itoh, Hiroyuki
Alvarez, Gladis Susana
Saito, Tadao
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Jensenii
Porcine Antigen Presenting Cells
Anti-Inflammatory Activity
Toll-Like Receptor 4
topic Lactobacillus Jensenii
Porcine Antigen Presenting Cells
Anti-Inflammatory Activity
Toll-Like Receptor 4
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Suzuki, Rie. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Takahashi, Takuya. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Tomosada, Yohsuke. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimazu, Tomoyuki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Ohwada, Shuichi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Saito, Tadao. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Kitazawa, Haruki. Tohuku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
description Previously, we demonstrated that Lactobacillus (L.) jensenii TL2937 attenuates the inflammatory response triggered by activation of toll-like receptor (TLR)-4 in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cells (APCs) polarization in immunoregulation, the objective of the present study was to examine the effect of TL2937 strain on activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs to L. jensenii in the absence of inflammatory signals increased expression of IL-10 and TGF-β in CD172a+ APCs and caused them to display tolerogenic properties. In addition, pre-treatment of CD172a+ APCs with L. jensenii resulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of TL2937 strain was not related to a downregulation of TLR4 but an upregulation of the expression of three negative regulators of TLRs: SIGIRR, A20, and IRAK-M. Our results also indicated that TLR2 has an important role in the anti-inflammatory activity of L. jensenii TL2937 since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+ APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+ cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+ cells harvested from swine PPs were useful for in vitro study of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.
publishDate 2012
dc.date.none.fl_str_mv 2012-05-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24931
Villena, Julio Cesar; Suzuki, Rie; Fujie, Hitomi; Chiba, Eriko; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells; American Society for Microbiology; Clinical And Vaccine Immunology; 19; 7; 9-5-2012; 1038-1053
1556-6811
1556-679X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24931
identifier_str_mv Villena, Julio Cesar; Suzuki, Rie; Fujie, Hitomi; Chiba, Eriko; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus jensenii modulates toll-like receptor 4-induced inflammatory response via negative regulation in porcine antigen presenting cells; American Society for Microbiology; Clinical And Vaccine Immunology; 19; 7; 9-5-2012; 1038-1053
1556-6811
1556-679X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/CVI.00199-12
info:eu-repo/semantics/altIdentifier/url/http://cvi.asm.org/content/19/7/1038
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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