Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells

Autores
Tsukida, Kohichiro; Takahashi, Takuya; Iida, Hikaru; Kanmani, Paulraj; Suda, Yoshihito; Nochi, Tomonori; Ohwada, Shuichi; Aso, Hisashi; Ohkawara, Sou; Makino, Seiya; Kano, Hiroshi; Saito, Tadao; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer?s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. Objective: In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Results: studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Conclusions: Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.
Fil: Tsukida, Kohichiro. Tohoku University; Japón
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Ohwada, Shuichi. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural & Veterinary Division; Japón
Fil: Makino, Seiya. Meiji Co., Ltd. Division of Research and Development; Japón
Fil: Kano, Hiroshi. Meiji Co., Ltd. Division of Research and Development; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
LACTOBACILLUS JENSENII TL2937
PORCINE ANTIGEN PRESENTING CELLS
PHAGOCYTOSIS
IMMUNOBIOTICS
BLOOD MONOCYTES-DERIVED DENTRITIC CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80202
Acceder
id CONICETDig_6b387a9005e1acaca22d4888a62bb7d0
oai_identifier_str oai:ri.conicet.gov.ar:11336/80202
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cellsTsukida, KohichiroTakahashi, TakuyaIida, HikaruKanmani, PaulrajSuda, YoshihitoNochi, TomonoriOhwada, ShuichiAso, HisashiOhkawara, SouMakino, SeiyaKano, HiroshiSaito, TadaoVillena, Julio CesarKitazawa, HarukiLACTOBACILLUS JENSENII TL2937PORCINE ANTIGEN PRESENTING CELLSPHAGOCYTOSISIMMUNOBIOTICSBLOOD MONOCYTES-DERIVED DENTRITIC CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer?s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. Objective: In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Results: studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Conclusions: Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.Fil: Tsukida, Kohichiro. Tohoku University; JapónFil: Takahashi, Takuya. Tohoku University; JapónFil: Iida, Hikaru. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Ohwada, Shuichi. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural & Veterinary Division; JapónFil: Makino, Seiya. Meiji Co., Ltd. Division of Research and Development; JapónFil: Kano, Hiroshi. Meiji Co., Ltd. Division of Research and Development; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónBioMed Central2016-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80202Tsukida, Kohichiro; Takahashi, Takuya; Iida, Hikaru; Kanmani, Paulraj; Suda, Yoshihito; et al.; Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells; BioMed Central; BMC Immunology; 17; 21; 24-6-2016; 1-121471-21721471-2172CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12865-016-0160-1info:eu-repo/semantics/altIdentifier/url/https://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-016-0160-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:15Zoai:ri.conicet.gov.ar:11336/80202instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:15.292CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
title Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
spellingShingle Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
Tsukida, Kohichiro
LACTOBACILLUS JENSENII TL2937
PORCINE ANTIGEN PRESENTING CELLS
PHAGOCYTOSIS
IMMUNOBIOTICS
BLOOD MONOCYTES-DERIVED DENTRITIC CELLS
title_short Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
title_full Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
title_fullStr Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
title_full_unstemmed Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
title_sort Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells
dc.creator.none.fl_str_mv Tsukida, Kohichiro
Takahashi, Takuya
Iida, Hikaru
Kanmani, Paulraj
Suda, Yoshihito
Nochi, Tomonori
Ohwada, Shuichi
Aso, Hisashi
Ohkawara, Sou
Makino, Seiya
Kano, Hiroshi
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author Tsukida, Kohichiro
author_facet Tsukida, Kohichiro
Takahashi, Takuya
Iida, Hikaru
Kanmani, Paulraj
Suda, Yoshihito
Nochi, Tomonori
Ohwada, Shuichi
Aso, Hisashi
Ohkawara, Sou
Makino, Seiya
Kano, Hiroshi
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Takahashi, Takuya
Iida, Hikaru
Kanmani, Paulraj
Suda, Yoshihito
Nochi, Tomonori
Ohwada, Shuichi
Aso, Hisashi
Ohkawara, Sou
Makino, Seiya
Kano, Hiroshi
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS JENSENII TL2937
PORCINE ANTIGEN PRESENTING CELLS
PHAGOCYTOSIS
IMMUNOBIOTICS
BLOOD MONOCYTES-DERIVED DENTRITIC CELLS
topic LACTOBACILLUS JENSENII TL2937
PORCINE ANTIGEN PRESENTING CELLS
PHAGOCYTOSIS
IMMUNOBIOTICS
BLOOD MONOCYTES-DERIVED DENTRITIC CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer?s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. Objective: In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Results: studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Conclusions: Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.
Fil: Tsukida, Kohichiro. Tohoku University; Japón
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Ohwada, Shuichi. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural & Veterinary Division; Japón
Fil: Makino, Seiya. Meiji Co., Ltd. Division of Research and Development; Japón
Fil: Kano, Hiroshi. Meiji Co., Ltd. Division of Research and Development; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Background: Immunobiotic Lactobacillus jensenii TL2937 modulates porcine mononuclear phagocytes from Peyer?s patches (PPMPs) and induces a differential production of pro- and anti-inflammatory cytokines in response to Toll-like receptor (TLR)-4 activation. Objective: In view of the important role played by phagocytosis in the activation of antigen presenting cells (APCs), the aim of the present work was to examine the interaction of TL2937 with porcine PPMPs focusing on phagocytosis. In addition, this study aimed to investigate whether the effects of L. jensenii TL2937 in porcine blood monocyte-derived dendritic cells (MoDCs) are similar to those found in PPMPs considering that MoDCs do not recapitulate all functions of mucosal APCs. Results: studies showed a high ability of porcine CD172a+ PPMPs to phagocytose L. jensenii TL2937. Interestingly, our results also revealed a reduced capacity of the non-immunomodulatory L. plantarum TL2766 to be phagocytosed by those immune cells. Phagocytosis of L. jensenii TL2937 by porcine PPMPs was partially dependent on TLR2. In addition, we demonstrated that TL2937 strain was able to improve the expression of IL-1, IL-12 and IL-10 in immature MoDCs resembling the effect of this immunobiotic bacterium on PPMPs. Moreover, similarly to PPMPs those immunomodulatory effects were related to the higher capacity of TL2937 to be phagocytosed by immature MoDCs. Conclusions: Microbial recognition in APCs could be effectively mediated through ligand-receptor interactions that then mediate phagocytosis and signaling. For the immunobiotic strain TL2937, TLR2 has a partial role for its interaction with porcine APCs and it is necessary to investigate the role of other receptors. A challenge for future research will be advance in the full understanding of the molecular interactions of immunobiotic L. jensenii TL2937 with porcine APCs that will be crucial for the successful development of functional feeds for the porcine host. This study is a step in that direction.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80202
Tsukida, Kohichiro; Takahashi, Takuya; Iida, Hikaru; Kanmani, Paulraj; Suda, Yoshihito; et al.; Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells; BioMed Central; BMC Immunology; 17; 21; 24-6-2016; 1-12
1471-2172
1471-2172
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80202
identifier_str_mv Tsukida, Kohichiro; Takahashi, Takuya; Iida, Hikaru; Kanmani, Paulraj; Suda, Yoshihito; et al.; Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells; BioMed Central; BMC Immunology; 17; 21; 24-6-2016; 1-12
1471-2172
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s12865-016-0160-1
info:eu-repo/semantics/altIdentifier/url/https://bmcimmunol.biomedcentral.com/articles/10.1186/s12865-016-0160-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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