Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling...

Autores
Shimazu, Tomoyuki; Villena, Julio Cesar; Tohno, Masanori; Fujie, Hitomi; Hosoya, Shoichi; Shimosato, Takeshi; Aso, Hisashi; Suda, Yoshihito; Kawai, Yasushi; Saito, Tadao; Makino, Seiya; Ikegami, Shuji; Itoh, Hiroyuki; Kitazawa, Haruki
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited toll-like receptor (TLR)-4-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jensenii TL2937 attenuated the expression of pro-inflammatory cytokines and chemokines caused by ETEC or LPS challenge by downregulating TLR4-dependent nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. Furthermore, we demonstrated that L. jensenii TL2937 stimulation of PIE cells upregulated three negative regulators of TLRs: A20, Bcl-3 and MPK-1, deepening the understanding of an immunobiotic mechanism of action. L. jensenii TL2937-mediated induction of negative regulators of TLRs would have a substantial physiological impact on homeostasis in PIE cells because excessive TLR inflammatory signaling would be downregulated. These results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.
Fil: Shimazu, Tomoyuki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Tohno, Masanori. National Institute Of Livestock And Grassland Science; Japón
Fil: Fujie, Hitomi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Josai University. Graduate School of Pharmaceutical Science. Department of Nutritional Physiology; Japón
Fil: Hosoya, Shoichi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Shinshu University. Fiber-Nanotech Young Researcher Empowerment Center; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Kawai, Yasushi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Makino, Seiya. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Materia
Lactobacillus Jensenii Tl2937
Porcine Intestinal Epithelial Cells
Toll-Like Receptor 4
Anti-Inflammatory Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16996
Acceder
id CONICETDig_8e380e8ccc1642b574a657a06ccb007c
oai_identifier_str oai:ri.conicet.gov.ar:11336/16996
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathwayShimazu, TomoyukiVillena, Julio CesarTohno, MasanoriFujie, HitomiHosoya, ShoichiShimosato, TakeshiAso, HisashiSuda, YoshihitoKawai, YasushiSaito, TadaoMakino, SeiyaIkegami, ShujiItoh, HiroyukiKitazawa, HarukiLactobacillus Jensenii Tl2937Porcine Intestinal Epithelial CellsToll-Like Receptor 4Anti-Inflammatory Activityhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited toll-like receptor (TLR)-4-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jensenii TL2937 attenuated the expression of pro-inflammatory cytokines and chemokines caused by ETEC or LPS challenge by downregulating TLR4-dependent nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. Furthermore, we demonstrated that L. jensenii TL2937 stimulation of PIE cells upregulated three negative regulators of TLRs: A20, Bcl-3 and MPK-1, deepening the understanding of an immunobiotic mechanism of action. L. jensenii TL2937-mediated induction of negative regulators of TLRs would have a substantial physiological impact on homeostasis in PIE cells because excessive TLR inflammatory signaling would be downregulated. These results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.Fil: Shimazu, Tomoyuki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Villena, Julio Cesar. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Tohno, Masanori. National Institute Of Livestock And Grassland Science; JapónFil: Fujie, Hitomi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Josai University. Graduate School of Pharmaceutical Science. Department of Nutritional Physiology; JapónFil: Hosoya, Shoichi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Shimosato, Takeshi. Shinshu University. Fiber-Nanotech Young Researcher Empowerment Center; JapónFil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; JapónFil: Kawai, Yasushi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Saito, Tadao. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; JapónFil: Makino, Seiya. Meiji Dairies Corporation. Division of Research and Development; JapónFil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; JapónFil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; JapónFil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; JapónAmerican Society For Microbiology2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16996Shimazu, Tomoyuki; Villena, Julio Cesar; Tohno, Masanori; Fujie, Hitomi; Hosoya, Shoichi; et al.; Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway; American Society For Microbiology; Infection And Immunity; 80; 1; 1-2012; 276-2880019-95671098-5522enginfo:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.05729-11info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255675/info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/80/1/276info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:46Zoai:ri.conicet.gov.ar:11336/16996instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:46.676CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
title Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
spellingShingle Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
Shimazu, Tomoyuki
Lactobacillus Jensenii Tl2937
Porcine Intestinal Epithelial Cells
Toll-Like Receptor 4
Anti-Inflammatory Activity
title_short Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
title_full Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
title_fullStr Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
title_full_unstemmed Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
title_sort Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway
dc.creator.none.fl_str_mv Shimazu, Tomoyuki
Villena, Julio Cesar
Tohno, Masanori
Fujie, Hitomi
Hosoya, Shoichi
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Makino, Seiya
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author Shimazu, Tomoyuki
author_facet Shimazu, Tomoyuki
Villena, Julio Cesar
Tohno, Masanori
Fujie, Hitomi
Hosoya, Shoichi
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Makino, Seiya
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Tohno, Masanori
Fujie, Hitomi
Hosoya, Shoichi
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Makino, Seiya
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus Jensenii Tl2937
Porcine Intestinal Epithelial Cells
Toll-Like Receptor 4
Anti-Inflammatory Activity
topic Lactobacillus Jensenii Tl2937
Porcine Intestinal Epithelial Cells
Toll-Like Receptor 4
Anti-Inflammatory Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited toll-like receptor (TLR)-4-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jensenii TL2937 attenuated the expression of pro-inflammatory cytokines and chemokines caused by ETEC or LPS challenge by downregulating TLR4-dependent nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. Furthermore, we demonstrated that L. jensenii TL2937 stimulation of PIE cells upregulated three negative regulators of TLRs: A20, Bcl-3 and MPK-1, deepening the understanding of an immunobiotic mechanism of action. L. jensenii TL2937-mediated induction of negative regulators of TLRs would have a substantial physiological impact on homeostasis in PIE cells because excessive TLR inflammatory signaling would be downregulated. These results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.
Fil: Shimazu, Tomoyuki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Tohno, Masanori. National Institute Of Livestock And Grassland Science; Japón
Fil: Fujie, Hitomi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Josai University. Graduate School of Pharmaceutical Science. Department of Nutritional Physiology; Japón
Fil: Hosoya, Shoichi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Shinshu University. Fiber-Nanotech Young Researcher Empowerment Center; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Kawai, Yasushi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Makino, Seiya. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
description The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited toll-like receptor (TLR)-4-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jensenii TL2937 attenuated the expression of pro-inflammatory cytokines and chemokines caused by ETEC or LPS challenge by downregulating TLR4-dependent nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. Furthermore, we demonstrated that L. jensenii TL2937 stimulation of PIE cells upregulated three negative regulators of TLRs: A20, Bcl-3 and MPK-1, deepening the understanding of an immunobiotic mechanism of action. L. jensenii TL2937-mediated induction of negative regulators of TLRs would have a substantial physiological impact on homeostasis in PIE cells because excessive TLR inflammatory signaling would be downregulated. These results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16996
Shimazu, Tomoyuki; Villena, Julio Cesar; Tohno, Masanori; Fujie, Hitomi; Hosoya, Shoichi; et al.; Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway; American Society For Microbiology; Infection And Immunity; 80; 1; 1-2012; 276-288
0019-9567
1098-5522
url http://hdl.handle.net/11336/16996
identifier_str_mv Shimazu, Tomoyuki; Villena, Julio Cesar; Tohno, Masanori; Fujie, Hitomi; Hosoya, Shoichi; et al.; Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway; American Society For Microbiology; Infection And Immunity; 80; 1; 1-2012; 276-288
0019-9567
1098-5522
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.05729-11
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255675/
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/80/1/276
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society For Microbiology
publisher.none.fl_str_mv American Society For Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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