Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells

Autores
Villena, Julio Cesar; Chiba, Eriko; Vizoso Pinto, María Guadalupe; Tomosada, Yohsuke; Takahashi, Takuya; Ishizuka, Takuya; Aso, Takuya; Salva, Maria Susana; Alvarez, Gladis Susana; Kitazawa, Haruki
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. Objective: we aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer?s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. Results: L. rhamnosus CRL1505 was able to induce IFN?α and ?β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs. Conclusions: it was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Chiba, Eriko. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Ishizuka, Takuya. Tohoku University; Japón
Fil: Aso, Takuya. Tohoku University; Japón
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
LACTOBACILLUS RHAMNOSUS
POLY(I:C)
ANTIVIRAL IMMUNITY
PIE CELLS
INTESTINAL ANTIGEN PRESENTING CELLS
TLR2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102106
Acceder
id CONICETDig_dc88431678577f398e24ac162eb3ec1d
oai_identifier_str oai:ri.conicet.gov.ar:11336/102106
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cellsVillena, Julio CesarChiba, ErikoVizoso Pinto, María GuadalupeTomosada, YohsukeTakahashi, TakuyaIshizuka, TakuyaAso, TakuyaSalva, Maria SusanaAlvarez, Gladis SusanaKitazawa, HarukiLACTOBACILLUS RHAMNOSUSPOLY(I:C)ANTIVIRAL IMMUNITYPIE CELLSINTESTINAL ANTIGEN PRESENTING CELLSTLR2https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. Objective: we aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer?s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. Results: L. rhamnosus CRL1505 was able to induce IFN?α and ?β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs. Conclusions: it was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Chiba, Eriko. Tohoku University; JapónFil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Tomosada, Yohsuke. Tohoku University; JapónFil: Takahashi, Takuya. Tohoku University; JapónFil: Ishizuka, Takuya. Tohoku University; JapónFil: Aso, Takuya. Tohoku University; JapónFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Kitazawa, Haruki. Tohoku University; JapónBioMed Central2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102106Villena, Julio Cesar; Chiba, Eriko; Vizoso Pinto, María Guadalupe; Tomosada, Yohsuke; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells; BioMed Central; BMC Microbiology; 14; 126; 6-2014; 1-141471-21801471-2180CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-126info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-126info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:06Zoai:ri.conicet.gov.ar:11336/102106instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:07.076CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
title Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
spellingShingle Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
Villena, Julio Cesar
LACTOBACILLUS RHAMNOSUS
POLY(I:C)
ANTIVIRAL IMMUNITY
PIE CELLS
INTESTINAL ANTIGEN PRESENTING CELLS
TLR2
title_short Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
title_full Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
title_fullStr Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
title_full_unstemmed Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
title_sort Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells
dc.creator.none.fl_str_mv Villena, Julio Cesar
Chiba, Eriko
Vizoso Pinto, María Guadalupe
Tomosada, Yohsuke
Takahashi, Takuya
Ishizuka, Takuya
Aso, Takuya
Salva, Maria Susana
Alvarez, Gladis Susana
Kitazawa, Haruki
author Villena, Julio Cesar
author_facet Villena, Julio Cesar
Chiba, Eriko
Vizoso Pinto, María Guadalupe
Tomosada, Yohsuke
Takahashi, Takuya
Ishizuka, Takuya
Aso, Takuya
Salva, Maria Susana
Alvarez, Gladis Susana
Kitazawa, Haruki
author_role author
author2 Chiba, Eriko
Vizoso Pinto, María Guadalupe
Tomosada, Yohsuke
Takahashi, Takuya
Ishizuka, Takuya
Aso, Takuya
Salva, Maria Susana
Alvarez, Gladis Susana
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LACTOBACILLUS RHAMNOSUS
POLY(I:C)
ANTIVIRAL IMMUNITY
PIE CELLS
INTESTINAL ANTIGEN PRESENTING CELLS
TLR2
topic LACTOBACILLUS RHAMNOSUS
POLY(I:C)
ANTIVIRAL IMMUNITY
PIE CELLS
INTESTINAL ANTIGEN PRESENTING CELLS
TLR2
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. Objective: we aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer?s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. Results: L. rhamnosus CRL1505 was able to induce IFN?α and ?β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs. Conclusions: it was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Chiba, Eriko. Tohoku University; Japón
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Takahashi, Takuya. Tohoku University; Japón
Fil: Ishizuka, Takuya. Tohoku University; Japón
Fil: Aso, Takuya. Tohoku University; Japón
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Kitazawa, Haruki. Tohoku University; Japón
description Background: previous findings suggested that Lactobacillus rhamnosus CRL1505 is able to increase resistance of children to intestinal viral infections. However, the intestinal cells, cytokines and receptors involved in the immunoregulatory effect of this probiotic strain have not been fully characterized. Objective: we aimed to gain insight into the mechanisms involved in the immunomodulatory effect of the CRL1505 strain and therefore evaluated in vitro the crosstalk between L. rhamnosus CRL1505, porcine intestinal epithelial cells (IECs) and antigen presenting cells (APCs) from swine Peyer?s patches in order to deepen our knowledge about the mechanisms, through which this strain may help preventing viral diarrhoea episodes. Results: L. rhamnosus CRL1505 was able to induce IFN?α and ?β in IECs and improve the production of type I IFNs in response to poly(I:C) challenge independently of Toll-like receptor (TLR)-2 or TLR9 signalling. In addition, the CRL1505 strain induced mRNA expression of IL-6 and TNF-α via TLR2 in IECs. Furthermore, the strain significantly increased surface molecules expression and cytokine production in intestinal APCs. The improved Th1 response induced by L. rhamnosus CRL1505 was triggered by TLR2 signalling and included augmented expression of MHC-II and co-stimulatory molecules and expression of IL-1β, IL-6, and IFN-γ in APCs. IL-10 was also significantly up-regulated by CRL1505 in APCs. Conclusions: it was recently reviewed the emergence of TLR agonists as new ways to transform antiviral treatments by introducing panviral therapeutics with less adverse effects than IFN therapies. The use of L. rhamnosus CRL1505 as modulator of innate immunity and inductor of antiviral type I IFNs, IFN-γ, and regulatory IL-10 clearly offers the potential to overcome this challenge.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102106
Villena, Julio Cesar; Chiba, Eriko; Vizoso Pinto, María Guadalupe; Tomosada, Yohsuke; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells; BioMed Central; BMC Microbiology; 14; 126; 6-2014; 1-14
1471-2180
1471-2180
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102106
identifier_str_mv Villena, Julio Cesar; Chiba, Eriko; Vizoso Pinto, María Guadalupe; Tomosada, Yohsuke; Takahashi, Takuya; et al.; Immunobiotic Lactobacillus rhamnosus strains differentially modulate antiviral immune response in intestinal epithelial and antigen presenting cells; BioMed Central; BMC Microbiology; 14; 126; 6-2014; 1-14
1471-2180
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-14-126
info:eu-repo/semantics/altIdentifier/url/https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-126
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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