Airway structural cells regulate TLR5-mediated mucosal adjuvant activity

Autores
Van Maele, Laurye; Fougeron, Delphine; Janot, Laurent; Didierlaurent, A.; Cayet, D.; Tabareau, J.; Rumbo, Martín; Corvo Chamaillard, S.; Boulenoir, S.; Jeffs, S; Vande Walle, L; Lamkanfi, M.; Lemoine, Y.; Erard, F.; Hot, D.; Hussell, Tracy; Ryffel, B.; Benecke, Arndt G.; Sirard, J.C.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antigen-presenting cell (APC) activation is enhanced by vaccine adjuvants. Most vaccines are based on the assumption that adjuvant activity of Toll-like receptor (TLR) agonists depends on direct, functional activation of APCs. Here, we sought to establish whether TLR stimulation in non-hematopoietic cells contributes to flagellin’s mucosal adjuvant activity. Nasal administration of flagellin enhanced T-cell-mediated immunity, and systemic and secretory antibody responses to coadministered antigens in a TLR5-dependent manner. Mucosal adjuvant activity was not affected by either abrogation of TLR5 signaling in hematopoietic cells or the presence of flagellin-specific, circulating neutralizing antibodies. We found that flagellin is rapidly degraded in conducting airways, does not translocate into lung parenchyma and stimulates an early immune response, suggesting that TLR5 signaling is regionalized. The flagellin-specific early response of lung was regulated by radioresistant cells expressing TLR5 (particularly the airway epithelial cells). Flagellin stimulated the epithelial production of a small set of mediators that included the chemokine CCL20, which is known to promote APC recruitment in mucosal tissues. Our data suggest that (i) the adjuvant activity of TLR agonists in mucosal vaccination may require TLR stimulation of structural cells and (ii) harnessing the effect of adjuvants on epithelial cells can improve mucosal vaccines.
Fil: Van Maele, Laurye. Institut Pasteur de Lille. Lille; Francia. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Fougeron, Delphine. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Janot, Laurent. University of Orléans. Orléans; Francia. Institut de Transgenose. Orleans; Francia
Fil: Didierlaurent, A.. Imperial College of London. Londres; Reino Unido
Fil: Cayet, D.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Tabareau, J.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Corvo Chamaillard, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Boulenoir, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Jeffs, S. Imperial College of London. Londres; Reino Unido
Fil: Vande Walle, L. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; Bélgica
Fil: Lamkanfi, M.. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; Bélgica
Fil: Lemoine, Y.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
Fil: Erard, F.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; Francia
Fil: Hot, D.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
Fil: Hussell, Tracy. Imperial College of London. Londres; Reino Unido. University of Manchester; Reino Unido
Fil: Ryffel, B.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; Francia
Fil: Benecke, Arndt G.. Institut des Hautes Études Scientifiques and Centre National de la Recherche Scientifique; Francia
Fil: Sirard, J.C.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
Materia
Adaptive Immunity
Antigen-Presenting Cells
Mucosal Immunology
Toll-Like Receptors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/32310
Acceder
id CONICETDig_0647bcae732aa99df9e2ed0ba78dc7d1
oai_identifier_str oai:ri.conicet.gov.ar:11336/32310
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Airway structural cells regulate TLR5-mediated mucosal adjuvant activityVan Maele, LauryeFougeron, DelphineJanot, LaurentDidierlaurent, A.Cayet, D.Tabareau, J.Rumbo, MartínCorvo Chamaillard, S.Boulenoir, S.Jeffs, SVande Walle, LLamkanfi, M.Lemoine, Y.Erard, F.Hot, D.Hussell, TracyRyffel, B.Benecke, Arndt G.Sirard, J.C.Adaptive ImmunityAntigen-Presenting CellsMucosal ImmunologyToll-Like Receptorshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Antigen-presenting cell (APC) activation is enhanced by vaccine adjuvants. Most vaccines are based on the assumption that adjuvant activity of Toll-like receptor (TLR) agonists depends on direct, functional activation of APCs. Here, we sought to establish whether TLR stimulation in non-hematopoietic cells contributes to flagellin’s mucosal adjuvant activity. Nasal administration of flagellin enhanced T-cell-mediated immunity, and systemic and secretory antibody responses to coadministered antigens in a TLR5-dependent manner. Mucosal adjuvant activity was not affected by either abrogation of TLR5 signaling in hematopoietic cells or the presence of flagellin-specific, circulating neutralizing antibodies. We found that flagellin is rapidly degraded in conducting airways, does not translocate into lung parenchyma and stimulates an early immune response, suggesting that TLR5 signaling is regionalized. The flagellin-specific early response of lung was regulated by radioresistant cells expressing TLR5 (particularly the airway epithelial cells). Flagellin stimulated the epithelial production of a small set of mediators that included the chemokine CCL20, which is known to promote APC recruitment in mucosal tissues. Our data suggest that (i) the adjuvant activity of TLR agonists in mucosal vaccination may require TLR stimulation of structural cells and (ii) harnessing the effect of adjuvants on epithelial cells can improve mucosal vaccines.Fil: Van Maele, Laurye. Institut Pasteur de Lille. Lille; Francia. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; FranciaFil: Fougeron, Delphine. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; FranciaFil: Janot, Laurent. University of Orléans. Orléans; Francia. Institut de Transgenose. Orleans; FranciaFil: Didierlaurent, A.. Imperial College of London. Londres; Reino UnidoFil: Cayet, D.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; FranciaFil: Tabareau, J.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; FranciaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Corvo Chamaillard, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; FranciaFil: Boulenoir, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; FranciaFil: Jeffs, S. Imperial College of London. Londres; Reino UnidoFil: Vande Walle, L. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; BélgicaFil: Lamkanfi, M.. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; BélgicaFil: Lemoine, Y.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; FranciaFil: Erard, F.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; FranciaFil: Hot, D.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; FranciaFil: Hussell, Tracy. Imperial College of London. Londres; Reino Unido. University of Manchester; Reino UnidoFil: Ryffel, B.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; FranciaFil: Benecke, Arndt G.. Institut des Hautes Études Scientifiques and Centre National de la Recherche Scientifique; FranciaFil: Sirard, J.C.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; FranciaNature Publishing Group2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/32310Van Maele, Laurye; Fougeron, Delphine; Janot, Laurent; Didierlaurent, A.; Cayet, D.; et al.; Airway structural cells regulate TLR5-mediated mucosal adjuvant activity; Nature Publishing Group; Mucosal Immunology; 7; 5-2014; 489-5001933-0219CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/mi.2013.66info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/mi201366info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:08Zoai:ri.conicet.gov.ar:11336/32310instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:08.727CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
title Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
spellingShingle Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
Van Maele, Laurye
Adaptive Immunity
Antigen-Presenting Cells
Mucosal Immunology
Toll-Like Receptors
title_short Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
title_full Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
title_fullStr Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
title_full_unstemmed Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
title_sort Airway structural cells regulate TLR5-mediated mucosal adjuvant activity
dc.creator.none.fl_str_mv Van Maele, Laurye
Fougeron, Delphine
Janot, Laurent
Didierlaurent, A.
Cayet, D.
Tabareau, J.
Rumbo, Martín
Corvo Chamaillard, S.
Boulenoir, S.
Jeffs, S
Vande Walle, L
Lamkanfi, M.
Lemoine, Y.
Erard, F.
Hot, D.
Hussell, Tracy
Ryffel, B.
Benecke, Arndt G.
Sirard, J.C.
author Van Maele, Laurye
author_facet Van Maele, Laurye
Fougeron, Delphine
Janot, Laurent
Didierlaurent, A.
Cayet, D.
Tabareau, J.
Rumbo, Martín
Corvo Chamaillard, S.
Boulenoir, S.
Jeffs, S
Vande Walle, L
Lamkanfi, M.
Lemoine, Y.
Erard, F.
Hot, D.
Hussell, Tracy
Ryffel, B.
Benecke, Arndt G.
Sirard, J.C.
author_role author
author2 Fougeron, Delphine
Janot, Laurent
Didierlaurent, A.
Cayet, D.
Tabareau, J.
Rumbo, Martín
Corvo Chamaillard, S.
Boulenoir, S.
Jeffs, S
Vande Walle, L
Lamkanfi, M.
Lemoine, Y.
Erard, F.
Hot, D.
Hussell, Tracy
Ryffel, B.
Benecke, Arndt G.
Sirard, J.C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adaptive Immunity
Antigen-Presenting Cells
Mucosal Immunology
Toll-Like Receptors
topic Adaptive Immunity
Antigen-Presenting Cells
Mucosal Immunology
Toll-Like Receptors
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Antigen-presenting cell (APC) activation is enhanced by vaccine adjuvants. Most vaccines are based on the assumption that adjuvant activity of Toll-like receptor (TLR) agonists depends on direct, functional activation of APCs. Here, we sought to establish whether TLR stimulation in non-hematopoietic cells contributes to flagellin’s mucosal adjuvant activity. Nasal administration of flagellin enhanced T-cell-mediated immunity, and systemic and secretory antibody responses to coadministered antigens in a TLR5-dependent manner. Mucosal adjuvant activity was not affected by either abrogation of TLR5 signaling in hematopoietic cells or the presence of flagellin-specific, circulating neutralizing antibodies. We found that flagellin is rapidly degraded in conducting airways, does not translocate into lung parenchyma and stimulates an early immune response, suggesting that TLR5 signaling is regionalized. The flagellin-specific early response of lung was regulated by radioresistant cells expressing TLR5 (particularly the airway epithelial cells). Flagellin stimulated the epithelial production of a small set of mediators that included the chemokine CCL20, which is known to promote APC recruitment in mucosal tissues. Our data suggest that (i) the adjuvant activity of TLR agonists in mucosal vaccination may require TLR stimulation of structural cells and (ii) harnessing the effect of adjuvants on epithelial cells can improve mucosal vaccines.
Fil: Van Maele, Laurye. Institut Pasteur de Lille. Lille; Francia. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Fougeron, Delphine. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Janot, Laurent. University of Orléans. Orléans; Francia. Institut de Transgenose. Orleans; Francia
Fil: Didierlaurent, A.. Imperial College of London. Londres; Reino Unido
Fil: Cayet, D.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Tabareau, J.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Corvo Chamaillard, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Boulenoir, S.. Institut Pasteur de Lille. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Univ Lille Nord de France. Lille; Francia
Fil: Jeffs, S. Imperial College of London. Londres; Reino Unido
Fil: Vande Walle, L. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; Bélgica
Fil: Lamkanfi, M.. Department of Medical Protein Research. Ghent; Bélgica. University of Ghent; Bélgica
Fil: Lemoine, Y.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
Fil: Erard, F.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; Francia
Fil: Hot, D.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
Fil: Hussell, Tracy. Imperial College of London. Londres; Reino Unido. University of Manchester; Reino Unido
Fil: Ryffel, B.. Institut de Transgenose. Orleans; Francia. University of Orléans. Orléans; Francia
Fil: Benecke, Arndt G.. Institut des Hautes Études Scientifiques and Centre National de la Recherche Scientifique; Francia
Fil: Sirard, J.C.. Univ Lille Nord de France. Lille; Francia. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Lille. Lille; Francia
description Antigen-presenting cell (APC) activation is enhanced by vaccine adjuvants. Most vaccines are based on the assumption that adjuvant activity of Toll-like receptor (TLR) agonists depends on direct, functional activation of APCs. Here, we sought to establish whether TLR stimulation in non-hematopoietic cells contributes to flagellin’s mucosal adjuvant activity. Nasal administration of flagellin enhanced T-cell-mediated immunity, and systemic and secretory antibody responses to coadministered antigens in a TLR5-dependent manner. Mucosal adjuvant activity was not affected by either abrogation of TLR5 signaling in hematopoietic cells or the presence of flagellin-specific, circulating neutralizing antibodies. We found that flagellin is rapidly degraded in conducting airways, does not translocate into lung parenchyma and stimulates an early immune response, suggesting that TLR5 signaling is regionalized. The flagellin-specific early response of lung was regulated by radioresistant cells expressing TLR5 (particularly the airway epithelial cells). Flagellin stimulated the epithelial production of a small set of mediators that included the chemokine CCL20, which is known to promote APC recruitment in mucosal tissues. Our data suggest that (i) the adjuvant activity of TLR agonists in mucosal vaccination may require TLR stimulation of structural cells and (ii) harnessing the effect of adjuvants on epithelial cells can improve mucosal vaccines.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/32310
Van Maele, Laurye; Fougeron, Delphine; Janot, Laurent; Didierlaurent, A.; Cayet, D.; et al.; Airway structural cells regulate TLR5-mediated mucosal adjuvant activity; Nature Publishing Group; Mucosal Immunology; 7; 5-2014; 489-500
1933-0219
CONICET Digital
CONICET
url http://hdl.handle.net/11336/32310
identifier_str_mv Van Maele, Laurye; Fougeron, Delphine; Janot, Laurent; Didierlaurent, A.; Cayet, D.; et al.; Airway structural cells regulate TLR5-mediated mucosal adjuvant activity; Nature Publishing Group; Mucosal Immunology; 7; 5-2014; 489-500
1933-0219
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/mi.2013.66
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/mi201366
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269386573873152
score 13.13397

Publicaciones similares