X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding

Autores
He, Weiguo; Elizondo Riojas, Miguel Angel; Li, Xin; Lokesh, Ganesh Lakshmana Rao; Somasunderam, Anoma; Thiviyanathan, Varatharasa; Volk, David E.; Durland, Ross H.; Englehardt, Johnnie; Cavasotto, Claudio Norberto; Gorenstein, David G.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold.
Fil: He, Weiguo. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Elizondo Riojas, Miguel Angel. University of Texas Health Science Center at Houston; Estados Unidos. Universidad Autónoma de Nuevo León. Hospital Universitario “Dr. José Eleuterio González”; México
Fil: Li, Xin. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Lokesh, Ganesh Lakshmana Rao. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Somasunderam, Anoma. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Thiviyanathan, Varatharasa. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Volk, David E.. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Durland, Ross H.. AM Biotechnologies; Estados Unidos
Fil: Englehardt, Johnnie. AM Biotechnologies; Estados Unidos
Fil: Cavasotto, Claudio Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Gorenstein, David G.. University of Texas Health Science Center at Houston; Estados Unidos
Materia
X-Aptamers
Structure-Based Drug Design
Molecular Dynamics
Cd44
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68063
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/68063
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced bindingHe, WeiguoElizondo Riojas, Miguel AngelLi, XinLokesh, Ganesh Lakshmana RaoSomasunderam, AnomaThiviyanathan, VaratharasaVolk, David E.Durland, Ross H.Englehardt, JohnnieCavasotto, Claudio NorbertoGorenstein, David G.X-AptamersStructure-Based Drug DesignMolecular DynamicsCd44https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold.Fil: He, Weiguo. University of Texas Health Science Center at Houston; Estados UnidosFil: Elizondo Riojas, Miguel Angel. University of Texas Health Science Center at Houston; Estados Unidos. Universidad Autónoma de Nuevo León. Hospital Universitario “Dr. José Eleuterio González”; MéxicoFil: Li, Xin. University of Texas Health Science Center at Houston; Estados UnidosFil: Lokesh, Ganesh Lakshmana Rao. University of Texas Health Science Center at Houston; Estados UnidosFil: Somasunderam, Anoma. University of Texas Health Science Center at Houston; Estados UnidosFil: Thiviyanathan, Varatharasa. University of Texas Health Science Center at Houston; Estados UnidosFil: Volk, David E.. University of Texas Health Science Center at Houston; Estados UnidosFil: Durland, Ross H.. AM Biotechnologies; Estados UnidosFil: Englehardt, Johnnie. AM Biotechnologies; Estados UnidosFil: Cavasotto, Claudio Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Gorenstein, David G.. University of Texas Health Science Center at Houston; Estados UnidosAmerican Chemical Society2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68063He, Weiguo; Elizondo Riojas, Miguel Angel; Li, Xin; Lokesh, Ganesh Lakshmana Rao; Somasunderam, Anoma; et al.; X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding; American Chemical Society; Biochemistry; 51; 42; 10-2012; 8321-83230006-2960CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/bi300471dinfo:eu-repo/semantics/altIdentifier/doi/10.1021/bi300471dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:08Zoai:ri.conicet.gov.ar:11336/68063instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:08.347CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
title X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
spellingShingle X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
He, Weiguo
X-Aptamers
Structure-Based Drug Design
Molecular Dynamics
Cd44
title_short X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
title_full X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
title_fullStr X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
title_full_unstemmed X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
title_sort X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding
dc.creator.none.fl_str_mv He, Weiguo
Elizondo Riojas, Miguel Angel
Li, Xin
Lokesh, Ganesh Lakshmana Rao
Somasunderam, Anoma
Thiviyanathan, Varatharasa
Volk, David E.
Durland, Ross H.
Englehardt, Johnnie
Cavasotto, Claudio Norberto
Gorenstein, David G.
author He, Weiguo
author_facet He, Weiguo
Elizondo Riojas, Miguel Angel
Li, Xin
Lokesh, Ganesh Lakshmana Rao
Somasunderam, Anoma
Thiviyanathan, Varatharasa
Volk, David E.
Durland, Ross H.
Englehardt, Johnnie
Cavasotto, Claudio Norberto
Gorenstein, David G.
author_role author
author2 Elizondo Riojas, Miguel Angel
Li, Xin
Lokesh, Ganesh Lakshmana Rao
Somasunderam, Anoma
Thiviyanathan, Varatharasa
Volk, David E.
Durland, Ross H.
Englehardt, Johnnie
Cavasotto, Claudio Norberto
Gorenstein, David G.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv X-Aptamers
Structure-Based Drug Design
Molecular Dynamics
Cd44
topic X-Aptamers
Structure-Based Drug Design
Molecular Dynamics
Cd44
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold.
Fil: He, Weiguo. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Elizondo Riojas, Miguel Angel. University of Texas Health Science Center at Houston; Estados Unidos. Universidad Autónoma de Nuevo León. Hospital Universitario “Dr. José Eleuterio González”; México
Fil: Li, Xin. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Lokesh, Ganesh Lakshmana Rao. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Somasunderam, Anoma. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Thiviyanathan, Varatharasa. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Volk, David E.. University of Texas Health Science Center at Houston; Estados Unidos
Fil: Durland, Ross H.. AM Biotechnologies; Estados Unidos
Fil: Englehardt, Johnnie. AM Biotechnologies; Estados Unidos
Fil: Cavasotto, Claudio Norberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Gorenstein, David G.. University of Texas Health Science Center at Houston; Estados Unidos
description By combining pseudorandom bead-based aptamer libraries with conjugation chemistry, we have created next-generation aptamers, X-aptamers (XAs). Several X-ligands can be added in a directed or random fashion to the aptamers to further enhance their binding affinities for the target proteins. Here we describe the addition of a drug (N-acetyl-2,3-dehydro-2-deoxyneuraminic acid), demonstrated to bind to CD44-HABD, to a complete monothioate backbone-substituted aptamer to increase its binding affinity for the target protein by up to 23-fold, while increasing the drug's level of binding 1-million fold.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68063
He, Weiguo; Elizondo Riojas, Miguel Angel; Li, Xin; Lokesh, Ganesh Lakshmana Rao; Somasunderam, Anoma; et al.; X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding; American Chemical Society; Biochemistry; 51; 42; 10-2012; 8321-8323
0006-2960
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68063
identifier_str_mv He, Weiguo; Elizondo Riojas, Miguel Angel; Li, Xin; Lokesh, Ganesh Lakshmana Rao; Somasunderam, Anoma; et al.; X-aptamers: A bead-based selection method for random incorporation of druglike moieties onto next-generation aptamers for enhanced binding; American Chemical Society; Biochemistry; 51; 42; 10-2012; 8321-8323
0006-2960
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/bi300471d
info:eu-repo/semantics/altIdentifier/doi/10.1021/bi300471d
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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