Optimization of Short RNA Aptamers for TNBC Cell Targeting

Autores
Camorani, Simona; D'Argenio, Annachiara; Agnello, Lisa; Nilo, Roberto; Zanetti, Antonella; Ibarra, Luis Exequiel; Fedele, Monica; Cerchia, Laura
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Triple-negative breast cancer (TNBC) is an aggressive cancer with limited targeted therapies. RNA aptamers, suitably chemically modified, work for therapeutic purposes in the same way as antibodies. We recently generated 2′ Fluoro-pyrimidines RNA-aptamers that act as effective recognition elements for functional surface signatures of TNBC cells. Here, we optimized three of them by shortening and proved the truncated aptamers as optimal candidates to enable active targeting to TNBC. By using prediction of secondary structure to guide truncation, we identified structural regions that account for the binding motifs of the full-length aptamers. Their chemical synthesis led to short aptamers with superb nuclease resistance, which specifically bind to TNBC target cells and rapidly internalize into acidic compartments. They interfere with the growth of TNBC cells as mammospheres, thus confirming their potential as anti-tumor agents. We propose sTN145, sTN58 and sTN29 aptamers as valuable tools for selective TNBC targeting and promising candidates for effective treatments, including therapeutic agents and targeted delivery nanovectors.
Fil: Camorani, Simona. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: D'Argenio, Annachiara. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Agnello, Lisa. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Nilo, Roberto. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Zanetti, Antonella. Institute Of Biostructures And Bioimaging; Italia
Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Fedele, Monica. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Cerchia, Laura. Institute Of Experimental Endocrinology And Oncology; Italia
Materia
ACTIVE TARGETING
APTAMER STRUCTURES
RNA APTAMER
TNBC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/202077
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Optimization of Short RNA Aptamers for TNBC Cell TargetingCamorani, SimonaD'Argenio, AnnachiaraAgnello, LisaNilo, RobertoZanetti, AntonellaIbarra, Luis ExequielFedele, MonicaCerchia, LauraACTIVE TARGETINGAPTAMER STRUCTURESRNA APTAMERTNBChttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Triple-negative breast cancer (TNBC) is an aggressive cancer with limited targeted therapies. RNA aptamers, suitably chemically modified, work for therapeutic purposes in the same way as antibodies. We recently generated 2′ Fluoro-pyrimidines RNA-aptamers that act as effective recognition elements for functional surface signatures of TNBC cells. Here, we optimized three of them by shortening and proved the truncated aptamers as optimal candidates to enable active targeting to TNBC. By using prediction of secondary structure to guide truncation, we identified structural regions that account for the binding motifs of the full-length aptamers. Their chemical synthesis led to short aptamers with superb nuclease resistance, which specifically bind to TNBC target cells and rapidly internalize into acidic compartments. They interfere with the growth of TNBC cells as mammospheres, thus confirming their potential as anti-tumor agents. We propose sTN145, sTN58 and sTN29 aptamers as valuable tools for selective TNBC targeting and promising candidates for effective treatments, including therapeutic agents and targeted delivery nanovectors.Fil: Camorani, Simona. Institute Of Experimental Endocrinology And Oncology; ItaliaFil: D'Argenio, Annachiara. Institute Of Experimental Endocrinology And Oncology; ItaliaFil: Agnello, Lisa. Institute Of Experimental Endocrinology And Oncology; ItaliaFil: Nilo, Roberto. Institute Of Experimental Endocrinology And Oncology; ItaliaFil: Zanetti, Antonella. Institute Of Biostructures And Bioimaging; ItaliaFil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; ArgentinaFil: Fedele, Monica. Institute Of Experimental Endocrinology And Oncology; ItaliaFil: Cerchia, Laura. Institute Of Experimental Endocrinology And Oncology; ItaliaMolecular Diversity Preservation International2022-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/202077Camorani, Simona; D'Argenio, Annachiara; Agnello, Lisa; Nilo, Roberto; Zanetti, Antonella; et al.; Optimization of Short RNA Aptamers for TNBC Cell Targeting; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 7; 4-2022; 1-181422-00671422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/7/3511info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms23073511info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:40Zoai:ri.conicet.gov.ar:11336/202077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:40.897CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimization of Short RNA Aptamers for TNBC Cell Targeting
title Optimization of Short RNA Aptamers for TNBC Cell Targeting
spellingShingle Optimization of Short RNA Aptamers for TNBC Cell Targeting
Camorani, Simona
ACTIVE TARGETING
APTAMER STRUCTURES
RNA APTAMER
TNBC
title_short Optimization of Short RNA Aptamers for TNBC Cell Targeting
title_full Optimization of Short RNA Aptamers for TNBC Cell Targeting
title_fullStr Optimization of Short RNA Aptamers for TNBC Cell Targeting
title_full_unstemmed Optimization of Short RNA Aptamers for TNBC Cell Targeting
title_sort Optimization of Short RNA Aptamers for TNBC Cell Targeting
dc.creator.none.fl_str_mv Camorani, Simona
D'Argenio, Annachiara
Agnello, Lisa
Nilo, Roberto
Zanetti, Antonella
Ibarra, Luis Exequiel
Fedele, Monica
Cerchia, Laura
author Camorani, Simona
author_facet Camorani, Simona
D'Argenio, Annachiara
Agnello, Lisa
Nilo, Roberto
Zanetti, Antonella
Ibarra, Luis Exequiel
Fedele, Monica
Cerchia, Laura
author_role author
author2 D'Argenio, Annachiara
Agnello, Lisa
Nilo, Roberto
Zanetti, Antonella
Ibarra, Luis Exequiel
Fedele, Monica
Cerchia, Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIVE TARGETING
APTAMER STRUCTURES
RNA APTAMER
TNBC
topic ACTIVE TARGETING
APTAMER STRUCTURES
RNA APTAMER
TNBC
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Triple-negative breast cancer (TNBC) is an aggressive cancer with limited targeted therapies. RNA aptamers, suitably chemically modified, work for therapeutic purposes in the same way as antibodies. We recently generated 2′ Fluoro-pyrimidines RNA-aptamers that act as effective recognition elements for functional surface signatures of TNBC cells. Here, we optimized three of them by shortening and proved the truncated aptamers as optimal candidates to enable active targeting to TNBC. By using prediction of secondary structure to guide truncation, we identified structural regions that account for the binding motifs of the full-length aptamers. Their chemical synthesis led to short aptamers with superb nuclease resistance, which specifically bind to TNBC target cells and rapidly internalize into acidic compartments. They interfere with the growth of TNBC cells as mammospheres, thus confirming their potential as anti-tumor agents. We propose sTN145, sTN58 and sTN29 aptamers as valuable tools for selective TNBC targeting and promising candidates for effective treatments, including therapeutic agents and targeted delivery nanovectors.
Fil: Camorani, Simona. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: D'Argenio, Annachiara. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Agnello, Lisa. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Nilo, Roberto. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Zanetti, Antonella. Institute Of Biostructures And Bioimaging; Italia
Fil: Ibarra, Luis Exequiel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Instituto de Biotecnologia Ambiental y Salud. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Biotecnologia Ambiental y Salud.; Argentina
Fil: Fedele, Monica. Institute Of Experimental Endocrinology And Oncology; Italia
Fil: Cerchia, Laura. Institute Of Experimental Endocrinology And Oncology; Italia
description Triple-negative breast cancer (TNBC) is an aggressive cancer with limited targeted therapies. RNA aptamers, suitably chemically modified, work for therapeutic purposes in the same way as antibodies. We recently generated 2′ Fluoro-pyrimidines RNA-aptamers that act as effective recognition elements for functional surface signatures of TNBC cells. Here, we optimized three of them by shortening and proved the truncated aptamers as optimal candidates to enable active targeting to TNBC. By using prediction of secondary structure to guide truncation, we identified structural regions that account for the binding motifs of the full-length aptamers. Their chemical synthesis led to short aptamers with superb nuclease resistance, which specifically bind to TNBC target cells and rapidly internalize into acidic compartments. They interfere with the growth of TNBC cells as mammospheres, thus confirming their potential as anti-tumor agents. We propose sTN145, sTN58 and sTN29 aptamers as valuable tools for selective TNBC targeting and promising candidates for effective treatments, including therapeutic agents and targeted delivery nanovectors.
publishDate 2022
dc.date.none.fl_str_mv 2022-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/202077
Camorani, Simona; D'Argenio, Annachiara; Agnello, Lisa; Nilo, Roberto; Zanetti, Antonella; et al.; Optimization of Short RNA Aptamers for TNBC Cell Targeting; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 7; 4-2022; 1-18
1422-0067
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/202077
identifier_str_mv Camorani, Simona; D'Argenio, Annachiara; Agnello, Lisa; Nilo, Roberto; Zanetti, Antonella; et al.; Optimization of Short RNA Aptamers for TNBC Cell Targeting; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 7; 4-2022; 1-18
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/7/3511
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms23073511
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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