Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina

Autores
Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle.
Fil: Ricatti, Maria Jimena. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Battista, Ariadna Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Faillace, Maria Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
CILIARY MARGINAL ZONE
EXTRACELLULAR NUCLEOTIDES
S-PHASE CELL RHYTHM
ZEBRAFISH RETINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18222
Acceder
id CONICETDig_e9149036fb0c4bc485ce5d4509372565
oai_identifier_str oai:ri.conicet.gov.ar:11336/18222
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retinaRicatti, Maria JimenaBattista, Ariadna GabrielaZorrilla Zubilete, María AureliaFaillace, Maria PaulaCILIARY MARGINAL ZONEEXTRACELLULAR NUCLEOTIDESS-PHASE CELL RHYTHMZEBRAFISH RETINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle.Fil: Ricatti, Maria Jimena. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Battista, Ariadna Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Faillace, Maria Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaSAGE Publications2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18222Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula; Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina; SAGE Publications; Journal of Biological Rhythms; 26; 2; 4-2011; 107-1170748-73041552-4531CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/0748730410395528info:eu-repo/semantics/altIdentifier/doi/10.1177/0748730410395528info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:39:31Zoai:ri.conicet.gov.ar:11336/18222instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:39:32.265CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
title Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
spellingShingle Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
Ricatti, Maria Jimena
CILIARY MARGINAL ZONE
EXTRACELLULAR NUCLEOTIDES
S-PHASE CELL RHYTHM
ZEBRAFISH RETINA
title_short Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
title_full Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
title_fullStr Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
title_full_unstemmed Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
title_sort Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
dc.creator.none.fl_str_mv Ricatti, Maria Jimena
Battista, Ariadna Gabriela
Zorrilla Zubilete, María Aurelia
Faillace, Maria Paula
author Ricatti, Maria Jimena
author_facet Ricatti, Maria Jimena
Battista, Ariadna Gabriela
Zorrilla Zubilete, María Aurelia
Faillace, Maria Paula
author_role author
author2 Battista, Ariadna Gabriela
Zorrilla Zubilete, María Aurelia
Faillace, Maria Paula
author2_role author
author
author
dc.subject.none.fl_str_mv CILIARY MARGINAL ZONE
EXTRACELLULAR NUCLEOTIDES
S-PHASE CELL RHYTHM
ZEBRAFISH RETINA
topic CILIARY MARGINAL ZONE
EXTRACELLULAR NUCLEOTIDES
S-PHASE CELL RHYTHM
ZEBRAFISH RETINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle.
Fil: Ricatti, Maria Jimena. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Battista, Ariadna Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Zorrilla Zubilete, María Aurelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Faillace, Maria Paula. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18222
Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula; Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina; SAGE Publications; Journal of Biological Rhythms; 26; 2; 4-2011; 107-117
0748-7304
1552-4531
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18222
identifier_str_mv Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula; Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina; SAGE Publications; Journal of Biological Rhythms; 26; 2; 4-2011; 107-117
0748-7304
1552-4531
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/abs/10.1177/0748730410395528
info:eu-repo/semantics/altIdentifier/doi/10.1177/0748730410395528
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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