Expression and cellular function of KCNQ channels in the eye
- Autores
- Vera, Marcela Sonia; Stupniki, Sofia; Barila, Esteban Pablo; German, Olga Lorena; Spitzmaul, Guillermo Federico
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- KCNQ subunits (1 to 5) form voltage-gated potassium channels. They are responsible of the M-current that regulates neuronal excitability in the CNS. KCNQ4 and -5 expression has been reported in the retina where could participate in the visual processing. Our aim was to investigate the expression and function of KCNQ channels in different tissues of the eye, employing KO mice for subunits 3 to 5. By KO-controlled immunofluorescence, we found a weak labeling of KCNQ4 in retinal pigmented epithelium (RPE) cells and the ciliary body (CB). KCNQ4 signal in RPE was stronger in the area adjacent to the CB and located in the apical membrane of the cells. In the CB the signal was placed in the basal membrane of the pigmented epithelium (PE) cells. KCNQ5 was found neither in the retina nor RPE or CB. Finally, KCNQ3 was observed in non-pigmented epithelium (NPE) cells of the CB. Gene expression analysis by RT-PCR showed that all KCNQ subunits were present in RPE/retinal tissue while in CB were only KCNQ1, -4 and - 5. This profile was altered in KCNQ4 KO mouse with the appearance of KCNQ2. By patch-clamp, we observed the M-current in 40% of the RPE cells while it was absent in the KCNQ4 KO mouse. In CB, the M-current was present in NPE cells, however it could not be analyzed in PE cells. NPE cell-currents were preserved in KCNQ4 KO mouse. In conclusion, KCNQ4 may participate in the homeostasis of K+ in the subretinal space and the ciliary body, contributing to transepithelial transport.
Fil: Vera, Marcela Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias - Materia
-
KCNQ4
RETINA
POTASSIUM
CILIARY-BODY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/192240
Ver los metadatos del registro completo
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Expression and cellular function of KCNQ channels in the eyeVera, Marcela SoniaStupniki, SofiaBarila, Esteban PabloGerman, Olga LorenaSpitzmaul, Guillermo FedericoKCNQ4RETINAPOTASSIUMCILIARY-BODYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1KCNQ subunits (1 to 5) form voltage-gated potassium channels. They are responsible of the M-current that regulates neuronal excitability in the CNS. KCNQ4 and -5 expression has been reported in the retina where could participate in the visual processing. Our aim was to investigate the expression and function of KCNQ channels in different tissues of the eye, employing KO mice for subunits 3 to 5. By KO-controlled immunofluorescence, we found a weak labeling of KCNQ4 in retinal pigmented epithelium (RPE) cells and the ciliary body (CB). KCNQ4 signal in RPE was stronger in the area adjacent to the CB and located in the apical membrane of the cells. In the CB the signal was placed in the basal membrane of the pigmented epithelium (PE) cells. KCNQ5 was found neither in the retina nor RPE or CB. Finally, KCNQ3 was observed in non-pigmented epithelium (NPE) cells of the CB. Gene expression analysis by RT-PCR showed that all KCNQ subunits were present in RPE/retinal tissue while in CB were only KCNQ1, -4 and - 5. This profile was altered in KCNQ4 KO mouse with the appearance of KCNQ2. By patch-clamp, we observed the M-current in 40% of the RPE cells while it was absent in the KCNQ4 KO mouse. In CB, the M-current was present in NPE cells, however it could not be analyzed in PE cells. NPE cell-currents were preserved in KCNQ4 KO mouse. In conclusion, KCNQ4 may participate in the homeostasis of K+ in the subretinal space and the ciliary body, contributing to transepithelial transport.Fil: Vera, Marcela Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaXXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019Villa Carlos PazArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192240Expression and cellular function of KCNQ channels in the eye; XXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019; Villa Carlos Paz; Argentina; 2019; 82-83CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/congresos-san-2/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:18Zoai:ri.conicet.gov.ar:11336/192240instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:18.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Expression and cellular function of KCNQ channels in the eye |
| title |
Expression and cellular function of KCNQ channels in the eye |
| spellingShingle |
Expression and cellular function of KCNQ channels in the eye Vera, Marcela Sonia KCNQ4 RETINA POTASSIUM CILIARY-BODY |
| title_short |
Expression and cellular function of KCNQ channels in the eye |
| title_full |
Expression and cellular function of KCNQ channels in the eye |
| title_fullStr |
Expression and cellular function of KCNQ channels in the eye |
| title_full_unstemmed |
Expression and cellular function of KCNQ channels in the eye |
| title_sort |
Expression and cellular function of KCNQ channels in the eye |
| dc.creator.none.fl_str_mv |
Vera, Marcela Sonia Stupniki, Sofia Barila, Esteban Pablo German, Olga Lorena Spitzmaul, Guillermo Federico |
| author |
Vera, Marcela Sonia |
| author_facet |
Vera, Marcela Sonia Stupniki, Sofia Barila, Esteban Pablo German, Olga Lorena Spitzmaul, Guillermo Federico |
| author_role |
author |
| author2 |
Stupniki, Sofia Barila, Esteban Pablo German, Olga Lorena Spitzmaul, Guillermo Federico |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
KCNQ4 RETINA POTASSIUM CILIARY-BODY |
| topic |
KCNQ4 RETINA POTASSIUM CILIARY-BODY |
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https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
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KCNQ subunits (1 to 5) form voltage-gated potassium channels. They are responsible of the M-current that regulates neuronal excitability in the CNS. KCNQ4 and -5 expression has been reported in the retina where could participate in the visual processing. Our aim was to investigate the expression and function of KCNQ channels in different tissues of the eye, employing KO mice for subunits 3 to 5. By KO-controlled immunofluorescence, we found a weak labeling of KCNQ4 in retinal pigmented epithelium (RPE) cells and the ciliary body (CB). KCNQ4 signal in RPE was stronger in the area adjacent to the CB and located in the apical membrane of the cells. In the CB the signal was placed in the basal membrane of the pigmented epithelium (PE) cells. KCNQ5 was found neither in the retina nor RPE or CB. Finally, KCNQ3 was observed in non-pigmented epithelium (NPE) cells of the CB. Gene expression analysis by RT-PCR showed that all KCNQ subunits were present in RPE/retinal tissue while in CB were only KCNQ1, -4 and - 5. This profile was altered in KCNQ4 KO mouse with the appearance of KCNQ2. By patch-clamp, we observed the M-current in 40% of the RPE cells while it was absent in the KCNQ4 KO mouse. In CB, the M-current was present in NPE cells, however it could not be analyzed in PE cells. NPE cell-currents were preserved in KCNQ4 KO mouse. In conclusion, KCNQ4 may participate in the homeostasis of K+ in the subretinal space and the ciliary body, contributing to transepithelial transport. Fil: Vera, Marcela Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Stupniki, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Barila, Esteban Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Spitzmaul, Guillermo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina XXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019 Villa Carlos Paz Argentina Sociedad Argentina de Investigación en Neurociencias |
| description |
KCNQ subunits (1 to 5) form voltage-gated potassium channels. They are responsible of the M-current that regulates neuronal excitability in the CNS. KCNQ4 and -5 expression has been reported in the retina where could participate in the visual processing. Our aim was to investigate the expression and function of KCNQ channels in different tissues of the eye, employing KO mice for subunits 3 to 5. By KO-controlled immunofluorescence, we found a weak labeling of KCNQ4 in retinal pigmented epithelium (RPE) cells and the ciliary body (CB). KCNQ4 signal in RPE was stronger in the area adjacent to the CB and located in the apical membrane of the cells. In the CB the signal was placed in the basal membrane of the pigmented epithelium (PE) cells. KCNQ5 was found neither in the retina nor RPE or CB. Finally, KCNQ3 was observed in non-pigmented epithelium (NPE) cells of the CB. Gene expression analysis by RT-PCR showed that all KCNQ subunits were present in RPE/retinal tissue while in CB were only KCNQ1, -4 and - 5. This profile was altered in KCNQ4 KO mouse with the appearance of KCNQ2. By patch-clamp, we observed the M-current in 40% of the RPE cells while it was absent in the KCNQ4 KO mouse. In CB, the M-current was present in NPE cells, however it could not be analyzed in PE cells. NPE cell-currents were preserved in KCNQ4 KO mouse. In conclusion, KCNQ4 may participate in the homeostasis of K+ in the subretinal space and the ciliary body, contributing to transepithelial transport. |
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2019 |
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2019 |
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http://hdl.handle.net/11336/192240 Expression and cellular function of KCNQ channels in the eye; XXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019; Villa Carlos Paz; Argentina; 2019; 82-83 CONICET Digital CONICET |
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Expression and cellular function of KCNQ channels in the eye; XXXIV Reunión Anual Sociedad Argentina de Investigación en Neurociencias 2019; Villa Carlos Paz; Argentina; 2019; 82-83 CONICET Digital CONICET |
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