Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock

Autores
Appelbaum, Lior; Vallone, Daniela; Anzulovich Miranda, Ana Cecilia; Ziv, Limor; Tom, Moshe; Foulkes, Nicholas S.; Gothilf, Yoav
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Daily rhythms of melatonin production are controlled by changes in the activity of arylalkylamine-N-acetyltransferase (AANAT). Zebrafish possess two aanats, aanat1 and aanat2; the former is expressed only in the retina and the latter is expressed in both the retina and the pineal gland. Here, their differential expression and regulation were studied using transcript quantification and transient and stable in vivo and in vitro transfection assays. In the pineal gland, the aanat2 promoter exhibited circadian clock-controlled activity, as indicated by circadian rhythms of Enhanced green f luorescent protein (EGFP) mRNA in AANAT2:EGFP transgenic fish. In vivo transient expression analyses of the aanat2 promoter indicated that E-box and photoreceptor conserved elements (PCE) are required for expression in the pineal gland. In the retina, the expression of both genes was characterized by a robust circadian rhythm of their transcript levels. In constant darkness, the rhythmic expression of retinal aanat2 persisted while the aanat1 rhythm disappeared; indicating that the former is controlled by a circadian clock and the latter is also light driven. In the light-entrainable clock-containing PAC-2 zebrafish cell line, both stably transfected aanat1 and aanat2 promoters exhibited a clock-controlled circadian rhythm, characteristic for an E-box-driven expression. Transient co-transfection experiments in NIH-3T3 cells revealed that the two, E-box- and PCE-containing, promoters are driven by the synergistic action of BMAL/CLOCK and orthehodenticle homeobox 5. This study has revealed a shared mechanism for the regulation of two related genes, yet describes their differential phases and photic responses which may be driven by other gene-specific regulatory mechanisms and tissue-specific transcription factor profiles.
Fil: Appelbaum, Lior. Universitat Tel Aviv; Israel. Bar-Ilan University. Faculty of Life Sciences; Israel
Fil: Vallone, Daniela. Max Planck Institut fur Entwicklungsbiologie; Alemania
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Ziv, Limor. Universitat Tel Aviv; Israel
Fil: Tom, Moshe. Israel Oceanographic and Limmnological Research; Israel
Fil: Foulkes, Nicholas S.. Max Planck Institut fur Entwicklungsbiologie; Alemania
Fil: Gothilf, Yoav. Universitat Tel Aviv; Israel
Materia
CIRCADIAN RHYTHM
A-NAT
ZEBRAFISH
TRANSCRIPTION REGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159773
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/159773
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clockAppelbaum, LiorVallone, DanielaAnzulovich Miranda, Ana CeciliaZiv, LimorTom, MosheFoulkes, Nicholas S.Gothilf, YoavCIRCADIAN RHYTHMA-NATZEBRAFISHTRANSCRIPTION REGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Daily rhythms of melatonin production are controlled by changes in the activity of arylalkylamine-N-acetyltransferase (AANAT). Zebrafish possess two aanats, aanat1 and aanat2; the former is expressed only in the retina and the latter is expressed in both the retina and the pineal gland. Here, their differential expression and regulation were studied using transcript quantification and transient and stable in vivo and in vitro transfection assays. In the pineal gland, the aanat2 promoter exhibited circadian clock-controlled activity, as indicated by circadian rhythms of Enhanced green f luorescent protein (EGFP) mRNA in AANAT2:EGFP transgenic fish. In vivo transient expression analyses of the aanat2 promoter indicated that E-box and photoreceptor conserved elements (PCE) are required for expression in the pineal gland. In the retina, the expression of both genes was characterized by a robust circadian rhythm of their transcript levels. In constant darkness, the rhythmic expression of retinal aanat2 persisted while the aanat1 rhythm disappeared; indicating that the former is controlled by a circadian clock and the latter is also light driven. In the light-entrainable clock-containing PAC-2 zebrafish cell line, both stably transfected aanat1 and aanat2 promoters exhibited a clock-controlled circadian rhythm, characteristic for an E-box-driven expression. Transient co-transfection experiments in NIH-3T3 cells revealed that the two, E-box- and PCE-containing, promoters are driven by the synergistic action of BMAL/CLOCK and orthehodenticle homeobox 5. This study has revealed a shared mechanism for the regulation of two related genes, yet describes their differential phases and photic responses which may be driven by other gene-specific regulatory mechanisms and tissue-specific transcription factor profiles.Fil: Appelbaum, Lior. Universitat Tel Aviv; Israel. Bar-Ilan University. Faculty of Life Sciences; IsraelFil: Vallone, Daniela. Max Planck Institut fur Entwicklungsbiologie; AlemaniaFil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Ziv, Limor. Universitat Tel Aviv; IsraelFil: Tom, Moshe. Israel Oceanographic and Limmnological Research; IsraelFil: Foulkes, Nicholas S.. Max Planck Institut fur Entwicklungsbiologie; AlemaniaFil: Gothilf, Yoav. Universitat Tel Aviv; IsraelBioScientifica2006-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159773Appelbaum, Lior; Vallone, Daniela; Anzulovich Miranda, Ana Cecilia; Ziv, Limor; Tom, Moshe; et al.; Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock; BioScientifica; Journal of Molecular Endocrinology; 36; 2; 4-2006; 337-3470952-5041CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://jme.bioscientifica.com/view/journals/jme/36/2/0360337.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1677/jme.1.01893info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:29Zoai:ri.conicet.gov.ar:11336/159773instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:30.175CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
title Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
spellingShingle Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
Appelbaum, Lior
CIRCADIAN RHYTHM
A-NAT
ZEBRAFISH
TRANSCRIPTION REGULATION
title_short Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
title_full Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
title_fullStr Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
title_full_unstemmed Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
title_sort Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock
dc.creator.none.fl_str_mv Appelbaum, Lior
Vallone, Daniela
Anzulovich Miranda, Ana Cecilia
Ziv, Limor
Tom, Moshe
Foulkes, Nicholas S.
Gothilf, Yoav
author Appelbaum, Lior
author_facet Appelbaum, Lior
Vallone, Daniela
Anzulovich Miranda, Ana Cecilia
Ziv, Limor
Tom, Moshe
Foulkes, Nicholas S.
Gothilf, Yoav
author_role author
author2 Vallone, Daniela
Anzulovich Miranda, Ana Cecilia
Ziv, Limor
Tom, Moshe
Foulkes, Nicholas S.
Gothilf, Yoav
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CIRCADIAN RHYTHM
A-NAT
ZEBRAFISH
TRANSCRIPTION REGULATION
topic CIRCADIAN RHYTHM
A-NAT
ZEBRAFISH
TRANSCRIPTION REGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Daily rhythms of melatonin production are controlled by changes in the activity of arylalkylamine-N-acetyltransferase (AANAT). Zebrafish possess two aanats, aanat1 and aanat2; the former is expressed only in the retina and the latter is expressed in both the retina and the pineal gland. Here, their differential expression and regulation were studied using transcript quantification and transient and stable in vivo and in vitro transfection assays. In the pineal gland, the aanat2 promoter exhibited circadian clock-controlled activity, as indicated by circadian rhythms of Enhanced green f luorescent protein (EGFP) mRNA in AANAT2:EGFP transgenic fish. In vivo transient expression analyses of the aanat2 promoter indicated that E-box and photoreceptor conserved elements (PCE) are required for expression in the pineal gland. In the retina, the expression of both genes was characterized by a robust circadian rhythm of their transcript levels. In constant darkness, the rhythmic expression of retinal aanat2 persisted while the aanat1 rhythm disappeared; indicating that the former is controlled by a circadian clock and the latter is also light driven. In the light-entrainable clock-containing PAC-2 zebrafish cell line, both stably transfected aanat1 and aanat2 promoters exhibited a clock-controlled circadian rhythm, characteristic for an E-box-driven expression. Transient co-transfection experiments in NIH-3T3 cells revealed that the two, E-box- and PCE-containing, promoters are driven by the synergistic action of BMAL/CLOCK and orthehodenticle homeobox 5. This study has revealed a shared mechanism for the regulation of two related genes, yet describes their differential phases and photic responses which may be driven by other gene-specific regulatory mechanisms and tissue-specific transcription factor profiles.
Fil: Appelbaum, Lior. Universitat Tel Aviv; Israel. Bar-Ilan University. Faculty of Life Sciences; Israel
Fil: Vallone, Daniela. Max Planck Institut fur Entwicklungsbiologie; Alemania
Fil: Anzulovich Miranda, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Ziv, Limor. Universitat Tel Aviv; Israel
Fil: Tom, Moshe. Israel Oceanographic and Limmnological Research; Israel
Fil: Foulkes, Nicholas S.. Max Planck Institut fur Entwicklungsbiologie; Alemania
Fil: Gothilf, Yoav. Universitat Tel Aviv; Israel
description Daily rhythms of melatonin production are controlled by changes in the activity of arylalkylamine-N-acetyltransferase (AANAT). Zebrafish possess two aanats, aanat1 and aanat2; the former is expressed only in the retina and the latter is expressed in both the retina and the pineal gland. Here, their differential expression and regulation were studied using transcript quantification and transient and stable in vivo and in vitro transfection assays. In the pineal gland, the aanat2 promoter exhibited circadian clock-controlled activity, as indicated by circadian rhythms of Enhanced green f luorescent protein (EGFP) mRNA in AANAT2:EGFP transgenic fish. In vivo transient expression analyses of the aanat2 promoter indicated that E-box and photoreceptor conserved elements (PCE) are required for expression in the pineal gland. In the retina, the expression of both genes was characterized by a robust circadian rhythm of their transcript levels. In constant darkness, the rhythmic expression of retinal aanat2 persisted while the aanat1 rhythm disappeared; indicating that the former is controlled by a circadian clock and the latter is also light driven. In the light-entrainable clock-containing PAC-2 zebrafish cell line, both stably transfected aanat1 and aanat2 promoters exhibited a clock-controlled circadian rhythm, characteristic for an E-box-driven expression. Transient co-transfection experiments in NIH-3T3 cells revealed that the two, E-box- and PCE-containing, promoters are driven by the synergistic action of BMAL/CLOCK and orthehodenticle homeobox 5. This study has revealed a shared mechanism for the regulation of two related genes, yet describes their differential phases and photic responses which may be driven by other gene-specific regulatory mechanisms and tissue-specific transcription factor profiles.
publishDate 2006
dc.date.none.fl_str_mv 2006-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159773
Appelbaum, Lior; Vallone, Daniela; Anzulovich Miranda, Ana Cecilia; Ziv, Limor; Tom, Moshe; et al.; Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock; BioScientifica; Journal of Molecular Endocrinology; 36; 2; 4-2006; 337-347
0952-5041
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159773
identifier_str_mv Appelbaum, Lior; Vallone, Daniela; Anzulovich Miranda, Ana Cecilia; Ziv, Limor; Tom, Moshe; et al.; Zebrafish arylalkylamine-N-acetyltransferase genes: targets for regulation of the circadian clock; BioScientifica; Journal of Molecular Endocrinology; 36; 2; 4-2006; 337-347
0952-5041
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://jme.bioscientifica.com/view/journals/jme/36/2/0360337.xml
info:eu-repo/semantics/altIdentifier/doi/10.1677/jme.1.01893
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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