Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range

Autores
Casero, Carina Noelia; Estévez-Braun, Ana; Gutiérrez Ravelo, Ángel; Demo, Mirta; Méndez Alvarez, Sabastián; Machin, Félix
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Currently, there is a pressing need for novel antibacterial agents against drug-resistant bacteria, especially those which have been common in our communities and hospitals, such as methicillin-resistant Staphylococcus aureus (MRSA). The South American plant Achyrocline satureioides (Marcela) has been widely used in traditional medicine for a number of diseases, including infections. Several crude extracts from this plant have shown good antimicrobial activities in vitro. In the search for the active principle (s) that confers these antimicrobial activities, we have processed the dichloromethane extract from the aerial parts of the plant. One of the isolated compounds showed extraordinary antibacterial activities against a set of clinically relevant Gram-positive strains that widely differ in their antibiogram profiles. This compound was identified as achyrofuran on the basis of its spectroscopic and physical data.
We determined the MIC to be around 0.1 M (0.07 g/ml) for the reference methicillin-resistant and
vancomycin-intermediate S. aureus strain NRS402. Moreover, nanomolar concentrations of  achyrofuran killed 10 6 bacteria within 12 h. Based on the presence of the 2,2-biphenol core, we further studied whether achyrofuran killed bacteria through a mechanism of action similar to that reported for the naturally occurring antibiotic MC21-A. Indeed, we found that achyrofuran was not bacteriolytic by itself although it greatly compromised membrane impermeability as determined by increased SYTOX Green uptake.
Fil: Casero, Carina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Rio Cuarto; Argentina. Universidad de la Laguna; España
Fil: Estévez-Braun, Ana. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Gutiérrez Ravelo, Ángel. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Demo, Mirta. Universidad Nacional de Rio Cuarto; Argentina
Fil: Méndez Alvarez, Sabastián. Universidad de la Laguna; España. Hospital Universitario Nuestra Señora de Candelaria; España
Fil: Machin, Félix. Hospital Universitario Nuestra Señora de Candelaria; España
Materia
Achyrocline Satureioides
Staphylococcus Aureus
Mrsa
Phytoalexins
Achyrofuran
Antimicrobial
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12235
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12235
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar rangeCasero, Carina NoeliaEstévez-Braun, AnaGutiérrez Ravelo, ÁngelDemo, MirtaMéndez Alvarez, SabastiánMachin, FélixAchyrocline SatureioidesStaphylococcus AureusMrsaPhytoalexinsAchyrofuranAntimicrobialhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Currently, there is a pressing need for novel antibacterial agents against drug-resistant bacteria, especially those which have been common in our communities and hospitals, such as methicillin-resistant Staphylococcus aureus (MRSA). The South American plant Achyrocline satureioides (Marcela) has been widely used in traditional medicine for a number of diseases, including infections. Several crude extracts from this plant have shown good antimicrobial activities in vitro. In the search for the active principle (s) that confers these antimicrobial activities, we have processed the dichloromethane extract from the aerial parts of the plant. One of the isolated compounds showed extraordinary antibacterial activities against a set of clinically relevant Gram-positive strains that widely differ in their antibiogram profiles. This compound was identified as achyrofuran on the basis of its spectroscopic and physical data.<br />We determined the MIC to be around 0.1 M (0.07 g/ml) for the reference methicillin-resistant and<br />vancomycin-intermediate S. aureus strain NRS402. Moreover, nanomolar concentrations of  achyrofuran killed 10 6 bacteria within 12 h. Based on the presence of the 2,2-biphenol core, we further studied whether achyrofuran killed bacteria through a mechanism of action similar to that reported for the naturally occurring antibiotic MC21-A. Indeed, we found that achyrofuran was not bacteriolytic by itself although it greatly compromised membrane impermeability as determined by increased SYTOX Green uptake.Fil: Casero, Carina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Rio Cuarto; Argentina. Universidad de la Laguna; EspañaFil: Estévez-Braun, Ana. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; EspañaFil: Gutiérrez Ravelo, Ángel. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; EspañaFil: Demo, Mirta. Universidad Nacional de Rio Cuarto; ArgentinaFil: Méndez Alvarez, Sabastián. Universidad de la Laguna; España. Hospital Universitario Nuestra Señora de Candelaria; EspañaFil: Machin, Félix. Hospital Universitario Nuestra Señora de Candelaria; EspañaElsevier Gmbh2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12235Casero, Carina Noelia; Estévez-Braun, Ana; Gutiérrez Ravelo, Ángel; Demo, Mirta; Méndez Alvarez, Sabastián; et al.; Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range; Elsevier Gmbh; Phytomedicine; 20; 2; 2-2013; 133-1380944-7113enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.11.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312004060info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:30:51Zoai:ri.conicet.gov.ar:11336/12235instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:30:51.478CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
title Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
spellingShingle Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
Casero, Carina Noelia
Achyrocline Satureioides
Staphylococcus Aureus
Mrsa
Phytoalexins
Achyrofuran
Antimicrobial
title_short Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
title_full Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
title_fullStr Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
title_full_unstemmed Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
title_sort Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range
dc.creator.none.fl_str_mv Casero, Carina Noelia
Estévez-Braun, Ana
Gutiérrez Ravelo, Ángel
Demo, Mirta
Méndez Alvarez, Sabastián
Machin, Félix
author Casero, Carina Noelia
author_facet Casero, Carina Noelia
Estévez-Braun, Ana
Gutiérrez Ravelo, Ángel
Demo, Mirta
Méndez Alvarez, Sabastián
Machin, Félix
author_role author
author2 Estévez-Braun, Ana
Gutiérrez Ravelo, Ángel
Demo, Mirta
Méndez Alvarez, Sabastián
Machin, Félix
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Achyrocline Satureioides
Staphylococcus Aureus
Mrsa
Phytoalexins
Achyrofuran
Antimicrobial
topic Achyrocline Satureioides
Staphylococcus Aureus
Mrsa
Phytoalexins
Achyrofuran
Antimicrobial
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Currently, there is a pressing need for novel antibacterial agents against drug-resistant bacteria, especially those which have been common in our communities and hospitals, such as methicillin-resistant Staphylococcus aureus (MRSA). The South American plant Achyrocline satureioides (Marcela) has been widely used in traditional medicine for a number of diseases, including infections. Several crude extracts from this plant have shown good antimicrobial activities in vitro. In the search for the active principle (s) that confers these antimicrobial activities, we have processed the dichloromethane extract from the aerial parts of the plant. One of the isolated compounds showed extraordinary antibacterial activities against a set of clinically relevant Gram-positive strains that widely differ in their antibiogram profiles. This compound was identified as achyrofuran on the basis of its spectroscopic and physical data.<br />We determined the MIC to be around 0.1 M (0.07 g/ml) for the reference methicillin-resistant and<br />vancomycin-intermediate S. aureus strain NRS402. Moreover, nanomolar concentrations of  achyrofuran killed 10 6 bacteria within 12 h. Based on the presence of the 2,2-biphenol core, we further studied whether achyrofuran killed bacteria through a mechanism of action similar to that reported for the naturally occurring antibiotic MC21-A. Indeed, we found that achyrofuran was not bacteriolytic by itself although it greatly compromised membrane impermeability as determined by increased SYTOX Green uptake.
Fil: Casero, Carina Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Rio Cuarto; Argentina. Universidad de la Laguna; España
Fil: Estévez-Braun, Ana. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Gutiérrez Ravelo, Ángel. Universidad de la Laguna; España. Instituto Canario de Investigación del Cáncer; España
Fil: Demo, Mirta. Universidad Nacional de Rio Cuarto; Argentina
Fil: Méndez Alvarez, Sabastián. Universidad de la Laguna; España. Hospital Universitario Nuestra Señora de Candelaria; España
Fil: Machin, Félix. Hospital Universitario Nuestra Señora de Candelaria; España
description Currently, there is a pressing need for novel antibacterial agents against drug-resistant bacteria, especially those which have been common in our communities and hospitals, such as methicillin-resistant Staphylococcus aureus (MRSA). The South American plant Achyrocline satureioides (Marcela) has been widely used in traditional medicine for a number of diseases, including infections. Several crude extracts from this plant have shown good antimicrobial activities in vitro. In the search for the active principle (s) that confers these antimicrobial activities, we have processed the dichloromethane extract from the aerial parts of the plant. One of the isolated compounds showed extraordinary antibacterial activities against a set of clinically relevant Gram-positive strains that widely differ in their antibiogram profiles. This compound was identified as achyrofuran on the basis of its spectroscopic and physical data.<br />We determined the MIC to be around 0.1 M (0.07 g/ml) for the reference methicillin-resistant and<br />vancomycin-intermediate S. aureus strain NRS402. Moreover, nanomolar concentrations of  achyrofuran killed 10 6 bacteria within 12 h. Based on the presence of the 2,2-biphenol core, we further studied whether achyrofuran killed bacteria through a mechanism of action similar to that reported for the naturally occurring antibiotic MC21-A. Indeed, we found that achyrofuran was not bacteriolytic by itself although it greatly compromised membrane impermeability as determined by increased SYTOX Green uptake.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12235
Casero, Carina Noelia; Estévez-Braun, Ana; Gutiérrez Ravelo, Ángel; Demo, Mirta; Méndez Alvarez, Sabastián; et al.; Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range; Elsevier Gmbh; Phytomedicine; 20; 2; 2-2013; 133-138
0944-7113
url http://hdl.handle.net/11336/12235
identifier_str_mv Casero, Carina Noelia; Estévez-Braun, Ana; Gutiérrez Ravelo, Ángel; Demo, Mirta; Méndez Alvarez, Sabastián; et al.; Achyrofuran is a strong antibacterial agent capable of killing Methicillin- Vancomycinresistant Staphylococcus aureus in the nanomolar range; Elsevier Gmbh; Phytomedicine; 20; 2; 2-2013; 133-138
0944-7113
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.11.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312004060
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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