Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina

Autores
Gardella, N.; Murzicato, S.; Di Gregorio, Sabrina Noelia; Cuirolo, Arabela Ximena; Desse, J.; Crudo, F.; Gutkind, Gabriel Osvaldo; Mollerach, Marta Eugenia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. Healthy carriers of S. aureus strains have an important role in the dissemination of this bacterium. The aim of this study was to estimate the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) carriage among healthy children in a city of Buenos Aires province, Argentina, and to determine the potential risk factors for its acquisition. We also described the molecular features of MRSA strains circulating in this population. S. aureus carriage was investigated in all children attending the last year of kindergarten during the 2008 school- year period. Household contacts of MRSA carriers were also screened. Of 316 healthy children, 98 (31.0%) carried S. aureus, including 14 MRSA carriers (4.4%) and 84 methicillin susceptible S. aureus (MSSA) carriers (26.6%). All MRSA isolates carried the SCCmec type IV cassette. Eight of the fourteen isolates were closely related to the clone responsible for most severe community- acquired MRSA infections caused in our country (CAA: PFGE A, SCCmec IV, spa t311, ST5). Two subtypes (A1 and A2) were distinguished in this group by PFGE. Both had agr type II and presented the same virulence determinants, except for PVL coding genes and sea that were only harbored by subtype A1. Our results, based on the analysis of MRSA isolates recovered in the screening of healthy children, provide evidence of a community reservoir of the major CA-MRSA clone described in Argentina.
Fil: Gardella, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Murzicato, S.. Hospital Municipal de San Antonio de Areco; Argentina
Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cuirolo, Arabela Ximena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Desse, J.. Hospital Paroissien; Argentina
Fil: Crudo, F.. Hospital Municipal de San Antonio de Areco; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
Staphylococcus Aureus
Mrsa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13309
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13309
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of ArgentinaGardella, N.Murzicato, S.Di Gregorio, Sabrina NoeliaCuirolo, Arabela XimenaDesse, J.Crudo, F.Gutkind, Gabriel OsvaldoMollerach, Marta EugeniaStaphylococcus AureusMrsahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. Healthy carriers of S. aureus strains have an important role in the dissemination of this bacterium. The aim of this study was to estimate the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) carriage among healthy children in a city of Buenos Aires province, Argentina, and to determine the potential risk factors for its acquisition. We also described the molecular features of MRSA strains circulating in this population. S. aureus carriage was investigated in all children attending the last year of kindergarten during the 2008 school- year period. Household contacts of MRSA carriers were also screened. Of 316 healthy children, 98 (31.0%) carried S. aureus, including 14 MRSA carriers (4.4%) and 84 methicillin susceptible S. aureus (MSSA) carriers (26.6%). All MRSA isolates carried the SCCmec type IV cassette. Eight of the fourteen isolates were closely related to the clone responsible for most severe community- acquired MRSA infections caused in our country (CAA: PFGE A, SCCmec IV, spa t311, ST5). Two subtypes (A1 and A2) were distinguished in this group by PFGE. Both had agr type II and presented the same virulence determinants, except for PVL coding genes and sea that were only harbored by subtype A1. Our results, based on the analysis of MRSA isolates recovered in the screening of healthy children, provide evidence of a community reservoir of the major CA-MRSA clone described in Argentina.Fil: Gardella, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Murzicato, S.. Hospital Municipal de San Antonio de Areco; ArgentinaFil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Cuirolo, Arabela Ximena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Desse, J.. Hospital Paroissien; ArgentinaFil: Crudo, F.. Hospital Municipal de San Antonio de Areco; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Science2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13309Gardella, N.; Murzicato, S.; Di Gregorio, Sabrina Noelia; Cuirolo, Arabela Ximena; Desse, J.; et al.; Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina; Elsevier Science; Infection, Genetics And Evolution; 11; 5; 5-2011; 1066-10711567-1348enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134811001006info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2011.03.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:52Zoai:ri.conicet.gov.ar:11336/13309instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:52.943CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
title Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
spellingShingle Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
Gardella, N.
Staphylococcus Aureus
Mrsa
title_short Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
title_full Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
title_fullStr Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
title_full_unstemmed Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
title_sort Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina
dc.creator.none.fl_str_mv Gardella, N.
Murzicato, S.
Di Gregorio, Sabrina Noelia
Cuirolo, Arabela Ximena
Desse, J.
Crudo, F.
Gutkind, Gabriel Osvaldo
Mollerach, Marta Eugenia
author Gardella, N.
author_facet Gardella, N.
Murzicato, S.
Di Gregorio, Sabrina Noelia
Cuirolo, Arabela Ximena
Desse, J.
Crudo, F.
Gutkind, Gabriel Osvaldo
Mollerach, Marta Eugenia
author_role author
author2 Murzicato, S.
Di Gregorio, Sabrina Noelia
Cuirolo, Arabela Ximena
Desse, J.
Crudo, F.
Gutkind, Gabriel Osvaldo
Mollerach, Marta Eugenia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Staphylococcus Aureus
Mrsa
topic Staphylococcus Aureus
Mrsa
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. Healthy carriers of S. aureus strains have an important role in the dissemination of this bacterium. The aim of this study was to estimate the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) carriage among healthy children in a city of Buenos Aires province, Argentina, and to determine the potential risk factors for its acquisition. We also described the molecular features of MRSA strains circulating in this population. S. aureus carriage was investigated in all children attending the last year of kindergarten during the 2008 school- year period. Household contacts of MRSA carriers were also screened. Of 316 healthy children, 98 (31.0%) carried S. aureus, including 14 MRSA carriers (4.4%) and 84 methicillin susceptible S. aureus (MSSA) carriers (26.6%). All MRSA isolates carried the SCCmec type IV cassette. Eight of the fourteen isolates were closely related to the clone responsible for most severe community- acquired MRSA infections caused in our country (CAA: PFGE A, SCCmec IV, spa t311, ST5). Two subtypes (A1 and A2) were distinguished in this group by PFGE. Both had agr type II and presented the same virulence determinants, except for PVL coding genes and sea that were only harbored by subtype A1. Our results, based on the analysis of MRSA isolates recovered in the screening of healthy children, provide evidence of a community reservoir of the major CA-MRSA clone described in Argentina.
Fil: Gardella, N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Murzicato, S.. Hospital Municipal de San Antonio de Areco; Argentina
Fil: Di Gregorio, Sabrina Noelia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Cuirolo, Arabela Ximena. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Desse, J.. Hospital Paroissien; Argentina
Fil: Crudo, F.. Hospital Municipal de San Antonio de Areco; Argentina
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Mollerach, Marta Eugenia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description Community acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is a major global problem. Healthy carriers of S. aureus strains have an important role in the dissemination of this bacterium. The aim of this study was to estimate the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) carriage among healthy children in a city of Buenos Aires province, Argentina, and to determine the potential risk factors for its acquisition. We also described the molecular features of MRSA strains circulating in this population. S. aureus carriage was investigated in all children attending the last year of kindergarten during the 2008 school- year period. Household contacts of MRSA carriers were also screened. Of 316 healthy children, 98 (31.0%) carried S. aureus, including 14 MRSA carriers (4.4%) and 84 methicillin susceptible S. aureus (MSSA) carriers (26.6%). All MRSA isolates carried the SCCmec type IV cassette. Eight of the fourteen isolates were closely related to the clone responsible for most severe community- acquired MRSA infections caused in our country (CAA: PFGE A, SCCmec IV, spa t311, ST5). Two subtypes (A1 and A2) were distinguished in this group by PFGE. Both had agr type II and presented the same virulence determinants, except for PVL coding genes and sea that were only harbored by subtype A1. Our results, based on the analysis of MRSA isolates recovered in the screening of healthy children, provide evidence of a community reservoir of the major CA-MRSA clone described in Argentina.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13309
Gardella, N.; Murzicato, S.; Di Gregorio, Sabrina Noelia; Cuirolo, Arabela Ximena; Desse, J.; et al.; Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina; Elsevier Science; Infection, Genetics And Evolution; 11; 5; 5-2011; 1066-1071
1567-1348
url http://hdl.handle.net/11336/13309
identifier_str_mv Gardella, N.; Murzicato, S.; Di Gregorio, Sabrina Noelia; Cuirolo, Arabela Ximena; Desse, J.; et al.; Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina; Elsevier Science; Infection, Genetics And Evolution; 11; 5; 5-2011; 1066-1071
1567-1348
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134811001006
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2011.03.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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